An LP1 analogue, selective MOR agonist with a peculiar pharmacological profile, used to scrutiny the ligand binding domain
Copyright © 2016 Elsevier Ltd. All rights reserved..
The hypothesis that central analgesia with reduced side effects is obtainable by occupying an 'allosteric' site in the MOR ligand binding domain requires the development of new ligands with peculiar pharmacological profile to be used as tools. New benzomorphan derivatives, analogues of LP1, a multitarget MOR agonist/DOR antagonist, were designed to examine in depth MOR ligand binding domain. Compound 5, bearing a diphenylic N-substituent on the benzomorphan nucleus, showed an affinity (Kiμ=0.5±0.2nM) comparable to that of LP1 and a better selectivity versus DOR and KOR. It elicits antinociceptive effects in ex vivo (GPI) and in vivo. This new compound engages receptor amino acidic residues not reached by LP1 and by other established MOR ligands. Molecular modeling studies, conducted on 5 and on several reference compounds, allowed us to propose possible residues in the MOR ligand binding domain essential for their interactions with 'orthosteric' and 'allosteric' binding sites.
| Medienart: |
E-Artikel |
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| Erscheinungsjahr: |
2016 |
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| Erschienen: |
2016 |
| Enthalten in: |
Zur Gesamtaufnahme - volume:24 |
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| Enthalten in: |
Bioorganic & medicinal chemistry - 24(2016), 21 vom: 01. Nov., Seite 5280-5290 |
| Sprache: |
Englisch |
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| Beteiligte Personen: |
Ronsisvalle, Simone [VerfasserIn] |
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| Links: |
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| Anmerkungen: |
Date Completed 14.08.2017 Date Revised 07.12.2017 published: Print-Electronic Citation Status MEDLINE |
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| doi: |
10.1016/j.bmc.2016.08.057 |
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| funding: |
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| Förderinstitution / Projekttitel: |
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NLM264305280 |
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| 100 | 1 | |a Ronsisvalle, Simone |e verfasserin |4 aut | |
| 245 | 1 | 3 | |a An LP1 analogue, selective MOR agonist with a peculiar pharmacological profile, used to scrutiny the ligand binding domain |
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| 500 | |a Date Revised 07.12.2017 | ||
| 500 | |a published: Print-Electronic | ||
| 500 | |a Citation Status MEDLINE | ||
| 520 | |a Copyright © 2016 Elsevier Ltd. All rights reserved. | ||
| 520 | |a The hypothesis that central analgesia with reduced side effects is obtainable by occupying an 'allosteric' site in the MOR ligand binding domain requires the development of new ligands with peculiar pharmacological profile to be used as tools. New benzomorphan derivatives, analogues of LP1, a multitarget MOR agonist/DOR antagonist, were designed to examine in depth MOR ligand binding domain. Compound 5, bearing a diphenylic N-substituent on the benzomorphan nucleus, showed an affinity (Kiμ=0.5±0.2nM) comparable to that of LP1 and a better selectivity versus DOR and KOR. It elicits antinociceptive effects in ex vivo (GPI) and in vivo. This new compound engages receptor amino acidic residues not reached by LP1 and by other established MOR ligands. Molecular modeling studies, conducted on 5 and on several reference compounds, allowed us to propose possible residues in the MOR ligand binding domain essential for their interactions with 'orthosteric' and 'allosteric' binding sites | ||
| 650 | 4 | |a Journal Article | |
| 650 | 4 | |a Research Support, Non-U.S. Gov't | |
| 650 | 4 | |a 6,7-Benzomorphan scaffold | |
| 650 | 4 | |a GPI and MVD assays | |
| 650 | 4 | |a MOR agonists | |
| 650 | 4 | |a Modeling studies | |
| 650 | 4 | |a Radioligand competition-binding assay | |
| 650 | 4 | |a Tail-flick test | |
| 650 | 7 | |a 3-((2R,6R,11R)-8-hydroxy-6,11-dimethyl-1,4,5,6-tetrahydro-2,6-methano-3-benzazocin-3(2H)-yl)-N-phenylpropanamide |2 NLM | |
| 650 | 7 | |a Benzomorphans |2 NLM | |
| 650 | 7 | |a Ligands |2 NLM | |
| 650 | 7 | |a Receptors, Opioid, mu |2 NLM | |
| 700 | 1 | |a Aricò, Giuseppina |e verfasserin |4 aut | |
| 700 | 1 | |a Panarello, Federica |e verfasserin |4 aut | |
| 700 | 1 | |a Spadaro, Angelo |e verfasserin |4 aut | |
| 700 | 1 | |a Pasquinucci, Lorella |e verfasserin |4 aut | |
| 700 | 1 | |a Pappalardo, Maria S |e verfasserin |4 aut | |
| 700 | 1 | |a Parenti, Carmela |e verfasserin |4 aut | |
| 700 | 1 | |a Ronsisvalle, Nicole |e verfasserin |4 aut | |
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