Simultaneous analysis of neutral monosaccharides, fatty acids and cholesterol as biomarkers from a drop of blood
AIM: We have developed a method for simultaneous monitoring of more biomarkers from three different classes of compounds by simultaneous analysis of neutral monosaccharides, fatty acids (FAs) and cholesterol as their per-O-methylated derivatives from a drop of blood by GC-MS. This work is a development of our previous results about analysis of neutral monosaccharides from a drop of blood.
METHODS & RESULTS: The simultaneous per-O-methylation was obtained by methylation in one step with methyl iodide and NaOH in DMSO. The per-O-methylated derivatives were separated in one chromatogram. The quantitative analysis was reproducible for five monosaccharides, 22 FAs and cholesterol. The results of this method were compared with those of the enzymatic methods using commercial kits.
CONCLUSION: This method can avoid the saponification of the FA methyl esters and can analyze for the first time simultaneously neutral monosaccharides, FAs and cholesterol from a drop of blood.
| Medienart: |
E-Artikel |
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| Erscheinungsjahr: |
2016 |
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| Erschienen: |
2016 |
| Enthalten in: |
Zur Gesamtaufnahme - volume:8 |
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| Enthalten in: |
Bioanalysis - 8(2016), 20 vom: 15. Okt., Seite 2147-56 |
| Sprache: |
Englisch |
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| Beteiligte Personen: |
Ciucanu, Ionel [VerfasserIn] |
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| Links: |
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| Anmerkungen: |
Date Completed 24.01.2017 Date Revised 24.01.2017 published: Print-Electronic Citation Status MEDLINE |
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| doi: |
10.4155/bio-2016-0107 |
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| funding: |
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| Förderinstitution / Projekttitel: |
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| PPN (Katalog-ID): |
NLM264192893 |
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| 100 | 1 | |a Ciucanu, Ionel |e verfasserin |4 aut | |
| 245 | 1 | 0 | |a Simultaneous analysis of neutral monosaccharides, fatty acids and cholesterol as biomarkers from a drop of blood |
| 264 | 1 | |c 2016 | |
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| 500 | |a Date Completed 24.01.2017 | ||
| 500 | |a Date Revised 24.01.2017 | ||
| 500 | |a published: Print-Electronic | ||
| 500 | |a Citation Status MEDLINE | ||
| 520 | |a AIM: We have developed a method for simultaneous monitoring of more biomarkers from three different classes of compounds by simultaneous analysis of neutral monosaccharides, fatty acids (FAs) and cholesterol as their per-O-methylated derivatives from a drop of blood by GC-MS. This work is a development of our previous results about analysis of neutral monosaccharides from a drop of blood | ||
| 520 | |a METHODS & RESULTS: The simultaneous per-O-methylation was obtained by methylation in one step with methyl iodide and NaOH in DMSO. The per-O-methylated derivatives were separated in one chromatogram. The quantitative analysis was reproducible for five monosaccharides, 22 FAs and cholesterol. The results of this method were compared with those of the enzymatic methods using commercial kits | ||
| 520 | |a CONCLUSION: This method can avoid the saponification of the FA methyl esters and can analyze for the first time simultaneously neutral monosaccharides, FAs and cholesterol from a drop of blood | ||
| 650 | 4 | |a Journal Article | |
| 650 | 4 | |a blood | |
| 650 | 4 | |a cholesterol | |
| 650 | 4 | |a disease-related biomarkers | |
| 650 | 4 | |a fatty acids | |
| 650 | 4 | |a monosaccharides | |
| 650 | 4 | |a simultaneous analysis | |
| 650 | 4 | |a simultaneous monitoring of multiple diseases | |
| 650 | 7 | |a Biomarkers |2 NLM | |
| 650 | 7 | |a Fatty Acids |2 NLM | |
| 650 | 7 | |a Hydrocarbons, Iodinated |2 NLM | |
| 650 | 7 | |a Monosaccharides |2 NLM | |
| 650 | 7 | |a Cholesterol |2 NLM | |
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| 650 | 7 | |a methyl iodide |2 NLM | |
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| 700 | 1 | |a Pilat, Luminiţa |e verfasserin |4 aut | |
| 700 | 1 | |a Ciucanu, Cristian I |e verfasserin |4 aut | |
| 700 | 1 | |a Şişu, Eugen |e verfasserin |4 aut | |
| 700 | 1 | |a Dumitraşcu, Victor |e verfasserin |4 aut | |
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