Synaptonemal Complex Components Are Required for Meiotic Checkpoint Function in Caenorhabditis elegans
Copyright © 2016 by the Genetics Society of America..
Synapsis involves the assembly of a proteinaceous structure, the synaptonemal complex (SC), between paired homologous chromosomes, and is essential for proper meiotic chromosome segregation. In Caenorhabditis elegans, the synapsis checkpoint selectively removes nuclei with unsynapsed chromosomes by inducing apoptosis. This checkpoint depends on pairing centers (PCs), cis-acting sites that promote pairing and synapsis. We have hypothesized that the stability of homolog pairing at PCs is monitored by this checkpoint. Here, we report that SC components SYP-3, HTP-3, HIM-3, and HTP-1 are required for a functional synapsis checkpoint. Mutation of these components does not abolish PC function, demonstrating they are bona fide checkpoint components. Further, we identify mutant backgrounds in which the instability of homolog pairing at PCs does not correlate with the synapsis checkpoint response. Altogether, these data suggest that, in addition to homolog pairing, SC assembly may be monitored by the synapsis checkpoint.
| Medienart: |
E-Artikel |
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| Erscheinungsjahr: |
2016 |
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| Erschienen: |
2016 |
| Enthalten in: |
Zur Gesamtaufnahme - volume:204 |
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| Enthalten in: |
Genetics - 204(2016), 3 vom: 12. Nov., Seite 987-997 |
| Sprache: |
Englisch |
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| Beteiligte Personen: |
Bohr, Tisha [VerfasserIn] |
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| Links: |
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| Anmerkungen: |
Date Completed 18.05.2017 Date Revised 29.11.2018 published: Print-Electronic Citation Status MEDLINE |
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| doi: |
10.1534/genetics.116.191494 |
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| funding: |
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| Förderinstitution / Projekttitel: |
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| PPN (Katalog-ID): |
NLM264137531 |
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| 500 | |a Date Completed 18.05.2017 | ||
| 500 | |a Date Revised 29.11.2018 | ||
| 500 | |a published: Print-Electronic | ||
| 500 | |a Citation Status MEDLINE | ||
| 520 | |a Copyright © 2016 by the Genetics Society of America. | ||
| 520 | |a Synapsis involves the assembly of a proteinaceous structure, the synaptonemal complex (SC), between paired homologous chromosomes, and is essential for proper meiotic chromosome segregation. In Caenorhabditis elegans, the synapsis checkpoint selectively removes nuclei with unsynapsed chromosomes by inducing apoptosis. This checkpoint depends on pairing centers (PCs), cis-acting sites that promote pairing and synapsis. We have hypothesized that the stability of homolog pairing at PCs is monitored by this checkpoint. Here, we report that SC components SYP-3, HTP-3, HIM-3, and HTP-1 are required for a functional synapsis checkpoint. Mutation of these components does not abolish PC function, demonstrating they are bona fide checkpoint components. Further, we identify mutant backgrounds in which the instability of homolog pairing at PCs does not correlate with the synapsis checkpoint response. Altogether, these data suggest that, in addition to homolog pairing, SC assembly may be monitored by the synapsis checkpoint | ||
| 650 | 4 | |a Journal Article | |
| 650 | 4 | |a Research Support, Non-U.S. Gov't | |
| 650 | 4 | |a Research Support, N.I.H., Extramural | |
| 650 | 4 | |a checkpoint | |
| 650 | 4 | |a chromosome | |
| 650 | 4 | |a meiosis | |
| 650 | 4 | |a synapsis | |
| 650 | 4 | |a synaptonemal complex | |
| 650 | 7 | |a Caenorhabditis elegans Proteins |2 NLM | |
| 650 | 7 | |a Cell Cycle Proteins |2 NLM | |
| 650 | 7 | |a Chromosomal Proteins, Non-Histone |2 NLM | |
| 650 | 7 | |a HIM-3 protein, C elegans |2 NLM | |
| 650 | 7 | |a HTP-3 protein, C elegans |2 NLM | |
| 650 | 7 | |a Htp-1 protein, C elegans |2 NLM | |
| 650 | 7 | |a SYP-3 protein, C elegans |2 NLM | |
| 700 | 1 | |a Ashley, Guinevere |e verfasserin |4 aut | |
| 700 | 1 | |a Eggleston, Evan |e verfasserin |4 aut | |
| 700 | 1 | |a Firestone, Kyra |e verfasserin |4 aut | |
| 700 | 1 | |a Bhalla, Needhi |e verfasserin |4 aut | |
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