Polymer surface properties control the function of heavy meromyosin in dynamic nanodevices
Copyright © 2016 Elsevier B.V. All rights reserved..
The actin-myosin system, responsible for muscle contraction, is also the force-generating element in dynamic nanodevices operating with surface-immobilized motor proteins. These devices require materials that are amenable to micro- and nano-fabrication, but also preserve the bioactivity of molecular motors. The complexity of the protein-surface systems is greatly amplified by those of the polymer-fluid interface; and of the structure and function of molecular motors, making the study of these interactions critical to the success of molecular motor-based nanodevices. We measured the density of the adsorbed motor protein (heavy meromyosin, HMM) using quartz crystal microbalance; and motor bioactivity with ATPase assay, on a set of model surfaces, i.e., nitrocellulose, polystyrene, poly(methyl methacrylate), and poly(butyl methacrylate), poly(tert-butyl methacrylate). A higher hydrophobicity of the adsorbing material translates in a higher total number of HMM molecules per unit area, but also in a lower uptake of water, and a lower ratio of active per total HMM molecules per unit area. We also measured the motility characteristics of actin filaments on the model surfaces, i.e., velocity, smoothness and deflection of movement, determined via in vitro motility assays. The filament velocities were found to be controlled by the relative number of active HMM per total motors, rather than their absolute surface density. The study allowed the formulation of the general engineering principles for the selection of polymeric materials for the manufacturing of dynamic nanodevices using protein molecular motors.
| Medienart: |
E-Artikel |
|---|
| Erscheinungsjahr: |
2017 |
|---|---|
| Erschienen: |
2017 |
| Enthalten in: |
Zur Gesamtaufnahme - volume:93 |
|---|---|
| Enthalten in: |
Biosensors & bioelectronics - 93(2017) vom: 15. Juli, Seite 305-314 |
| Sprache: |
Englisch |
|---|
| Beteiligte Personen: |
Hanson, Kristi L [VerfasserIn] |
|---|
| Links: |
|---|
| Themen: |
EC 3.6.4.1 |
|---|
| Anmerkungen: |
Date Completed 18.04.2017 Date Revised 18.04.2017 published: Print-Electronic Citation Status MEDLINE |
|---|
| doi: |
10.1016/j.bios.2016.08.061 |
|---|
| funding: |
|
|---|---|
| Förderinstitution / Projekttitel: |
|
| PPN (Katalog-ID): |
NLM264025598 |
|---|
| LEADER | 01000naa a22002652 4500 | ||
|---|---|---|---|
| 001 | NLM264025598 | ||
| 003 | DE-627 | ||
| 005 | 20231224205204.0 | ||
| 007 | cr uuu---uuuuu | ||
| 008 | 231224s2017 xx |||||o 00| ||eng c | ||
| 024 | 7 | |a 10.1016/j.bios.2016.08.061 |2 doi | |
| 028 | 5 | 2 | |a pubmed24n0880.xml |
| 035 | |a (DE-627)NLM264025598 | ||
| 035 | |a (NLM)27591903 | ||
| 035 | |a (PII)S0956-5663(16)30818-1 | ||
| 040 | |a DE-627 |b ger |c DE-627 |e rakwb | ||
| 041 | |a eng | ||
| 100 | 1 | |a Hanson, Kristi L |e verfasserin |4 aut | |
| 245 | 1 | 0 | |a Polymer surface properties control the function of heavy meromyosin in dynamic nanodevices |
| 264 | 1 | |c 2017 | |
| 336 | |a Text |b txt |2 rdacontent | ||
| 337 | |a ƒaComputermedien |b c |2 rdamedia | ||
| 338 | |a ƒa Online-Ressource |b cr |2 rdacarrier | ||
| 500 | |a Date Completed 18.04.2017 | ||
| 500 | |a Date Revised 18.04.2017 | ||
| 500 | |a published: Print-Electronic | ||
| 500 | |a Citation Status MEDLINE | ||
| 520 | |a Copyright © 2016 Elsevier B.V. All rights reserved. | ||
| 520 | |a The actin-myosin system, responsible for muscle contraction, is also the force-generating element in dynamic nanodevices operating with surface-immobilized motor proteins. These devices require materials that are amenable to micro- and nano-fabrication, but also preserve the bioactivity of molecular motors. The complexity of the protein-surface systems is greatly amplified by those of the polymer-fluid interface; and of the structure and function of molecular motors, making the study of these interactions critical to the success of molecular motor-based nanodevices. We measured the density of the adsorbed motor protein (heavy meromyosin, HMM) using quartz crystal microbalance; and motor bioactivity with ATPase assay, on a set of model surfaces, i.e., nitrocellulose, polystyrene, poly(methyl methacrylate), and poly(butyl methacrylate), poly(tert-butyl methacrylate). A higher hydrophobicity of the adsorbing material translates in a higher total number of HMM molecules per unit area, but also in a lower uptake of water, and a lower ratio of active per total HMM molecules per unit area. We also measured the motility characteristics of actin filaments on the model surfaces, i.e., velocity, smoothness and deflection of movement, determined via in vitro motility assays. The filament velocities were found to be controlled by the relative number of active HMM per total motors, rather than their absolute surface density. The study allowed the formulation of the general engineering principles for the selection of polymeric materials for the manufacturing of dynamic nanodevices using protein molecular motors | ||
| 650 | 4 | |a Journal Article | |
| 650 | 4 | |a Molecular motors | |
| 650 | 4 | |a Nanodevices | |
| 650 | 4 | |a Protein adsorption | |
| 650 | 7 | |a Myosin Subfragments |2 NLM | |
| 650 | 7 | |a Polymers |2 NLM | |
| 650 | 7 | |a Myosins |2 NLM | |
| 650 | 7 | |a EC 3.6.4.1 |2 NLM | |
| 700 | 1 | |a Fulga, Florin |e verfasserin |4 aut | |
| 700 | 1 | |a Dobroiu, Serban |e verfasserin |4 aut | |
| 700 | 1 | |a Solana, Gerardin |e verfasserin |4 aut | |
| 700 | 1 | |a Kaspar, Ondrej |e verfasserin |4 aut | |
| 700 | 1 | |a Tokarova, Viola |e verfasserin |4 aut | |
| 700 | 1 | |a Nicolau, Dan V |e verfasserin |4 aut | |
| 773 | 0 | 8 | |i Enthalten in |t Biosensors & bioelectronics |d 1996 |g 93(2017) vom: 15. Juli, Seite 305-314 |w (DE-627)NLM012733431 |x 1873-4235 |7 nnns |
| 773 | 1 | 8 | |g volume:93 |g year:2017 |g day:15 |g month:07 |g pages:305-314 |
| 856 | 4 | 0 | |u http://dx.doi.org/10.1016/j.bios.2016.08.061 |3 Volltext |
| 912 | |a GBV_USEFLAG_A | ||
| 912 | |a GBV_NLM | ||
| 951 | |a AR | ||
| 952 | |d 93 |j 2017 |b 15 |c 07 |h 305-314 | ||