Regulation of monocyte cell fate by blood vessels mediated by Notch signalling
A population of monocytes, known as Ly6C(lo) monocytes, patrol blood vessels by crawling along the vascular endothelium. Here we show that endothelial cells control their origin through Notch signalling. Using combinations of conditional genetic deletion strategies and cell-fate tracking experiments we show that Notch2 regulates conversion of Ly6C(hi) monocytes into Ly6C(lo) monocytes in vivo and in vitro, thereby regulating monocyte cell fate under steady-state conditions. This process is controlled by Notch ligand delta-like 1 (Dll1) expressed by a population of endothelial cells that constitute distinct vascular niches in the bone marrow and spleen in vivo, while culture on recombinant DLL1 induces monocyte conversion in vitro. Thus, blood vessels regulate monocyte conversion, a form of committed myeloid cell fate regulation.
| Errataetall: | |
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| Medienart: |
E-Artikel |
| Erscheinungsjahr: |
2016 |
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| Erschienen: |
2016 |
| Enthalten in: |
Zur Gesamtaufnahme - volume:7 |
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| Enthalten in: |
Nature communications - 7(2016) vom: 31. Aug., Seite 12597 |
| Sprache: |
Englisch |
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| Beteiligte Personen: |
Gamrekelashvili, Jaba [VerfasserIn] |
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| Links: |
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| Anmerkungen: |
Date Completed 07.09.2018 Date Revised 02.01.2021 published: Electronic ErratumIn: Nat Commun. 2017 May 03;8:15486. - PMID 28466869 Citation Status MEDLINE |
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| doi: |
10.1038/ncomms12597 |
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| funding: |
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| Förderinstitution / Projekttitel: |
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| PPN (Katalog-ID): |
NLM263891526 |
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| 245 | 1 | 0 | |a Regulation of monocyte cell fate by blood vessels mediated by Notch signalling |
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| 500 | |a published: Electronic | ||
| 500 | |a ErratumIn: Nat Commun. 2017 May 03;8:15486. - PMID 28466869 | ||
| 500 | |a Citation Status MEDLINE | ||
| 520 | |a A population of monocytes, known as Ly6C(lo) monocytes, patrol blood vessels by crawling along the vascular endothelium. Here we show that endothelial cells control their origin through Notch signalling. Using combinations of conditional genetic deletion strategies and cell-fate tracking experiments we show that Notch2 regulates conversion of Ly6C(hi) monocytes into Ly6C(lo) monocytes in vivo and in vitro, thereby regulating monocyte cell fate under steady-state conditions. This process is controlled by Notch ligand delta-like 1 (Dll1) expressed by a population of endothelial cells that constitute distinct vascular niches in the bone marrow and spleen in vivo, while culture on recombinant DLL1 induces monocyte conversion in vitro. Thus, blood vessels regulate monocyte conversion, a form of committed myeloid cell fate regulation | ||
| 650 | 4 | |a Journal Article | |
| 650 | 4 | |a Research Support, Non-U.S. Gov't | |
| 650 | 7 | |a Antigens, Ly |2 NLM | |
| 650 | 7 | |a Calcium-Binding Proteins |2 NLM | |
| 650 | 7 | |a Dlk1 protein, mouse |2 NLM | |
| 650 | 7 | |a FCGR3B protein, human |2 NLM | |
| 650 | 7 | |a GPI-Linked Proteins |2 NLM | |
| 650 | 7 | |a Intercellular Signaling Peptides and Proteins |2 NLM | |
| 650 | 7 | |a Ly-6C antigen, mouse |2 NLM | |
| 650 | 7 | |a NOTCH2 protein, human |2 NLM | |
| 650 | 7 | |a Notch2 protein, mouse |2 NLM | |
| 650 | 7 | |a Receptor, Notch2 |2 NLM | |
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| 700 | 1 | |a Briseño, Carlos G |e verfasserin |4 aut | |
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| 700 | 1 | |a Krishnasamy, Kashyap |e verfasserin |4 aut | |
| 700 | 1 | |a Limbourg, Anne |e verfasserin |4 aut | |
| 700 | 1 | |a Kapanadze, Tamar |e verfasserin |4 aut | |
| 700 | 1 | |a Ishifune, Chieko |e verfasserin |4 aut | |
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| 700 | 1 | |a Weber, Christian |e verfasserin |4 aut | |
| 700 | 1 | |a Limbourg, Florian P |e verfasserin |4 aut | |
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