Circulating (CD3(-)CD19(+)CD20(-)IgD(-)CD27(high)CD38(high)) Plasmablasts : A Promising Cellular Biomarker for Immune Activity for Anti-PLA2R1 Related Membranous Nephropathy?

Membranous nephropathy (MN) is a kidney specific autoimmune disease mainly mediated by anti-phospholipase A2 receptor 1 autoantibody (PLA2R1 Ab). The adequate assessment of chimeric anti-CD20 monoclonal antibody, rituximab (RTX), efficacy is still needed to improve clinical outcome of patient with MN. We evaluated the modification of plasmablasts (CD3(-)CD19(+)CD20(-)IgD(-)CD27(high)CD38(high)), a useful biomarker of RTX response in other autoimmune diseases, and memory (CD3(-)CD19(+)CD20(+)IgD(-)CD27(+)CD38(-)) and naive (CD3(-)CD19(+)CD20(+)IgD(+)CD27(-)CD38(low)) B cells by fluorescence-activated cell sorter analysis in PLA2R1 related MN in one patient during the 4 years of follow-up after RTX. RTX induced complete disappearance of CD19(+) B cells, plasmablasts, and memory B cells as soon as day 15. Despite severe CD19(+) lymphopenia, plasmablasts and memory B cells reemerged early before naive B cells (days 45, 90, and 120, resp.). During the follow-up, plasmablasts decreased more rapidly than memory B cells but still remained elevated as compared to day 0 of RTX. Concomitantly, anti-PLA2R1 Ab increased progressively. Our single case report suggests that, besides monitoring of serum anti-PLA2R1 Ab level, enumeration of circulating plasmablasts and memory B cells represents an attractive and complementary tool to assess immunological activity and efficacy of RTX induced B cells depletion in anti-PLA2R1 Ab related MN.

Media Type:

Electronic Article

Year of Publication:

2016

Contained In:

Mediators of inflammation - Vol. 2016 (2016), p. 7651024

Language:

English

Contributors:

Pozdzik, Agnieszka
Beukinga, Ingrid
Gu-Trantien, Chunyan
Willard-Gallo, Karen
Nortier, Joëlle
Pradier, Olivier

Links:

Volltext

Keywords:

ADP-ribosyl Cyclase 1
Antigens, CD19
Antigens, CD20
Biomarkers
CD3 Complex
EC 3.2.2.6
Glomerulonephritis, Membranous
Humans
Immunoglobulin D
Journal Article
Kidney
Male
Middle Aged
PLA2R1 protein, human
Plasma Cells
Receptors, Phospholipase A2
Tumor Necrosis Factor Receptor Superfamily, Member 7

Notes:

Date Completed 19.05.2017

Date Revised 13.11.2018

published: Print-Electronic

Citation Status MEDLINE

Copyright: From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine

Physical Description:

Online-Ressource

doi:

10.1155/2016/7651024

PMID:

27493452

PPN (Catalogue-ID):

NLM263179125