Extraction optimization, preliminary characterization and immunological activities in vitro of polysaccharides from Elaeagnus angustifolia L. pulp
Copyright © 2016 Elsevier Ltd. All rights reserved..
In this research, extraction optimization, preliminary characterization and immunological activities in vitro of polysaccharides from Elaeagnus angustifolia L. pulp were investigated. A response surface methodology (RSM) with a Box-Behnken design (BBD) was used to optimize the extraction process. The maximum EAP yield was 9.82±0.38%, which is in good agreement with the predicted value (9.93±0.24%). Two homogeneous polysaccharides, EAP-1a and EAP-1b with molecular weights of 8.70kDa and 4.39kDa respectively, were prepared by DEAE-52 cellulose and Sephadex G-100 columns and characterized by HPLC, HPGPC, and FT-IR. Three polysaccharides (EAP, EAP-1a and EAP-1b) could stimulate macrophages to release NO and enhance phagocytic activities of RAW 264.7 cells in dose-dependent manner. Moreover, there was no significant difference between crude EAP group (400μg/mL) and positive control group (LPS) in effects on macrophages. The results implied that EAP had the potential to be developed as natural medicines or health foods.
| Medienart: |
E-Artikel |
|---|
| Erscheinungsjahr: |
2016 |
|---|---|
| Erschienen: |
2016 |
| Enthalten in: |
Zur Gesamtaufnahme - volume:151 |
|---|---|
| Enthalten in: |
Carbohydrate polymers - 151(2016) vom: 20. Okt., Seite 348-357 |
| Sprache: |
Englisch |
|---|
| Beteiligte Personen: |
Du, Hongtao [VerfasserIn] |
|---|
| Links: |
|---|
| Themen: |
9014-76-0 |
|---|
| Anmerkungen: |
Date Completed 30.01.2018 Date Revised 07.04.2018 published: Print-Electronic Citation Status MEDLINE |
|---|
| doi: |
10.1016/j.carbpol.2016.05.068 |
|---|
| funding: |
|
|---|---|
| Förderinstitution / Projekttitel: |
|
| PPN (Katalog-ID): |
NLM262961997 |
|---|
| LEADER | 01000naa a22002652 4500 | ||
|---|---|---|---|
| 001 | NLM262961997 | ||
| 003 | DE-627 | ||
| 005 | 20231224202833.0 | ||
| 007 | cr uuu---uuuuu | ||
| 008 | 231224s2016 xx |||||o 00| ||eng c | ||
| 024 | 7 | |a 10.1016/j.carbpol.2016.05.068 |2 doi | |
| 028 | 5 | 2 | |a pubmed24n0876.xml |
| 035 | |a (DE-627)NLM262961997 | ||
| 035 | |a (NLM)27474576 | ||
| 035 | |a (PII)S0144-8617(16)30590-2 | ||
| 040 | |a DE-627 |b ger |c DE-627 |e rakwb | ||
| 041 | |a eng | ||
| 100 | 1 | |a Du, Hongtao |e verfasserin |4 aut | |
| 245 | 1 | 0 | |a Extraction optimization, preliminary characterization and immunological activities in vitro of polysaccharides from Elaeagnus angustifolia L. pulp |
| 264 | 1 | |c 2016 | |
| 336 | |a Text |b txt |2 rdacontent | ||
| 337 | |a ƒaComputermedien |b c |2 rdamedia | ||
| 338 | |a ƒa Online-Ressource |b cr |2 rdacarrier | ||
| 500 | |a Date Completed 30.01.2018 | ||
| 500 | |a Date Revised 07.04.2018 | ||
| 500 | |a published: Print-Electronic | ||
| 500 | |a Citation Status MEDLINE | ||
| 520 | |a Copyright © 2016 Elsevier Ltd. All rights reserved. | ||
| 520 | |a In this research, extraction optimization, preliminary characterization and immunological activities in vitro of polysaccharides from Elaeagnus angustifolia L. pulp were investigated. A response surface methodology (RSM) with a Box-Behnken design (BBD) was used to optimize the extraction process. The maximum EAP yield was 9.82±0.38%, which is in good agreement with the predicted value (9.93±0.24%). Two homogeneous polysaccharides, EAP-1a and EAP-1b with molecular weights of 8.70kDa and 4.39kDa respectively, were prepared by DEAE-52 cellulose and Sephadex G-100 columns and characterized by HPLC, HPGPC, and FT-IR. Three polysaccharides (EAP, EAP-1a and EAP-1b) could stimulate macrophages to release NO and enhance phagocytic activities of RAW 264.7 cells in dose-dependent manner. Moreover, there was no significant difference between crude EAP group (400μg/mL) and positive control group (LPS) in effects on macrophages. The results implied that EAP had the potential to be developed as natural medicines or health foods | ||
| 650 | 4 | |a Journal Article | |
| 650 | 4 | |a Characterization | |
| 650 | 4 | |a Elaeagnus angustifolia L. pulp | |
| 650 | 4 | |a Immunological activities | |
| 650 | 4 | |a Optimized extraction | |
| 650 | 4 | |a Polysaccharides | |
| 650 | 7 | |a Dextrans |2 NLM | |
| 650 | 7 | |a Polysaccharides |2 NLM | |
| 650 | 7 | |a sephadex |2 NLM | |
| 650 | 7 | |a 9014-76-0 |2 NLM | |
| 700 | 1 | |a Chen, Juncheng |e verfasserin |4 aut | |
| 700 | 1 | |a Tian, Shan |e verfasserin |4 aut | |
| 700 | 1 | |a Gu, Hongling |e verfasserin |4 aut | |
| 700 | 1 | |a Li, Na |e verfasserin |4 aut | |
| 700 | 1 | |a Sun, Yao |e verfasserin |4 aut | |
| 700 | 1 | |a Ru, Jiajia |e verfasserin |4 aut | |
| 700 | 1 | |a Wang, Junru |e verfasserin |4 aut | |
| 773 | 0 | 8 | |i Enthalten in |t Carbohydrate polymers |d 1994 |g 151(2016) vom: 20. Okt., Seite 348-357 |w (DE-627)NLM082819491 |x 1879-1344 |7 nnns |
| 773 | 1 | 8 | |g volume:151 |g year:2016 |g day:20 |g month:10 |g pages:348-357 |
| 856 | 4 | 0 | |u http://dx.doi.org/10.1016/j.carbpol.2016.05.068 |3 Volltext |
| 912 | |a GBV_USEFLAG_A | ||
| 912 | |a GBV_NLM | ||
| 951 | |a AR | ||
| 952 | |d 151 |j 2016 |b 20 |c 10 |h 348-357 | ||