Fast and comprehensive analysis of secondary metabolites in cocoa products using ultra high-performance liquid chromatography directly after pressurized liquid extraction
© 2016 WILEY-VCH Verlag GmbH & Co. KGaA, Weinheim..
Fast methods for the extraction and analysis of various secondary metabolites from cocoa products were developed and optimized regarding speed and separation efficiency. Extraction by pressurized liquid extraction is automated and the extracts are analyzed by rapid reversed-phase ultra high-performance liquid chromatography and normal-phase high-performance liquid chromatography methods. After extraction, no further sample treatment is required before chromatographic analysis. The analytes comprise monomeric and oligomeric flavanols, flavonols, methylxanthins, N-phenylpropenoyl amino acids, and phenolic acids. Polyphenols and N-phenylpropenoyl amino acids are separated in a single run of 33 min, procyanidins are analyzed by normal-phase high-performance liquid chromatography within 16 min, and methylxanthins require only 6 min total run time. A fourth method is suitable for phenolic acids, but only protocatechuic acid was found in relevant quantities. The optimized methods were validated and applied to 27 dark chocolates, one milk chocolate, two cocoa powders and two food supplements based on cocoa extract.
| Medienart: |
E-Artikel |
|---|
| Erscheinungsjahr: |
2016 |
|---|---|
| Erschienen: |
2016 |
| Enthalten in: |
Zur Gesamtaufnahme - volume:39 |
|---|---|
| Enthalten in: |
Journal of separation science - 39(2016), 16 vom: 14. Aug., Seite 3113-22 |
| Sprache: |
Englisch |
|---|
| Beteiligte Personen: |
Damm, Irina [VerfasserIn] |
|---|
| Links: |
|---|
| Themen: |
Chocolate |
|---|
| Anmerkungen: |
Date Completed 02.02.2018 Date Revised 18.03.2022 published: Print-Electronic Citation Status MEDLINE |
|---|
| doi: |
10.1002/jssc.201600422 |
|---|
| funding: |
|
|---|---|
| Förderinstitution / Projekttitel: |
|
| PPN (Katalog-ID): |
NLM262444941 |
|---|
| LEADER | 01000naa a22002652 4500 | ||
|---|---|---|---|
| 001 | NLM262444941 | ||
| 003 | DE-627 | ||
| 005 | 20231224201746.0 | ||
| 007 | cr uuu---uuuuu | ||
| 008 | 231224s2016 xx |||||o 00| ||eng c | ||
| 024 | 7 | |a 10.1002/jssc.201600422 |2 doi | |
| 028 | 5 | 2 | |a pubmed24n0874.xml |
| 035 | |a (DE-627)NLM262444941 | ||
| 035 | |a (NLM)27418182 | ||
| 040 | |a DE-627 |b ger |c DE-627 |e rakwb | ||
| 041 | |a eng | ||
| 100 | 1 | |a Damm, Irina |e verfasserin |4 aut | |
| 245 | 1 | 0 | |a Fast and comprehensive analysis of secondary metabolites in cocoa products using ultra high-performance liquid chromatography directly after pressurized liquid extraction |
| 264 | 1 | |c 2016 | |
| 336 | |a Text |b txt |2 rdacontent | ||
| 337 | |a ƒaComputermedien |b c |2 rdamedia | ||
| 338 | |a ƒa Online-Ressource |b cr |2 rdacarrier | ||
| 500 | |a Date Completed 02.02.2018 | ||
| 500 | |a Date Revised 18.03.2022 | ||
| 500 | |a published: Print-Electronic | ||
| 500 | |a Citation Status MEDLINE | ||
| 520 | |a © 2016 WILEY-VCH Verlag GmbH & Co. KGaA, Weinheim. | ||
| 520 | |a Fast methods for the extraction and analysis of various secondary metabolites from cocoa products were developed and optimized regarding speed and separation efficiency. Extraction by pressurized liquid extraction is automated and the extracts are analyzed by rapid reversed-phase ultra high-performance liquid chromatography and normal-phase high-performance liquid chromatography methods. After extraction, no further sample treatment is required before chromatographic analysis. The analytes comprise monomeric and oligomeric flavanols, flavonols, methylxanthins, N-phenylpropenoyl amino acids, and phenolic acids. Polyphenols and N-phenylpropenoyl amino acids are separated in a single run of 33 min, procyanidins are analyzed by normal-phase high-performance liquid chromatography within 16 min, and methylxanthins require only 6 min total run time. A fourth method is suitable for phenolic acids, but only protocatechuic acid was found in relevant quantities. The optimized methods were validated and applied to 27 dark chocolates, one milk chocolate, two cocoa powders and two food supplements based on cocoa extract | ||
| 650 | 4 | |a Evaluation Study | |
| 650 | 4 | |a Journal Article | |
| 650 | 4 | |a Chocolate | |
| 650 | 4 | |a Cocoa | |
| 650 | 4 | |a Flavanols | |
| 650 | 4 | |a Liquid chromatography | |
| 650 | 4 | |a Pressurized liquid extraction | |
| 650 | 7 | |a Plant Extracts |2 NLM | |
| 650 | 7 | |a Polyphenols |2 NLM | |
| 700 | 1 | |a Enger, Eileen |e verfasserin |4 aut | |
| 700 | 1 | |a Chrubasik-Hausmann, Sigrun |e verfasserin |4 aut | |
| 700 | 1 | |a Schieber, Andreas |e verfasserin |4 aut | |
| 700 | 1 | |a Zimmermann, Benno F |e verfasserin |4 aut | |
| 773 | 0 | 8 | |i Enthalten in |t Journal of separation science |d 2004 |g 39(2016), 16 vom: 14. Aug., Seite 3113-22 |w (DE-627)NLM150133529 |x 1615-9314 |7 nnns |
| 773 | 1 | 8 | |g volume:39 |g year:2016 |g number:16 |g day:14 |g month:08 |g pages:3113-22 |
| 856 | 4 | 0 | |u http://dx.doi.org/10.1002/jssc.201600422 |3 Volltext |
| 912 | |a GBV_USEFLAG_A | ||
| 912 | |a GBV_NLM | ||
| 951 | |a AR | ||
| 952 | |d 39 |j 2016 |e 16 |b 14 |c 08 |h 3113-22 | ||