Usefulness of a rapid intrapartum real-time PCR assay in comparison with the group B Streptococcus culture screening at the end of pregnancy in pregnant women
Copyright © 2016 Elsevier Masson SAS. All rights reserved..
OBJECTIVES: The objectives were to evaluate and compare the diagnostic accuracy of a rapid real-time PCR assay at the onset of labor with those of the current antenatal culture-based test at 34-38 weeks gestation for group B Streptococcus (GBS) screening.
MATERIALS AND METHODS: A prospective study including all pregnant women admitted for delivery after a 34-week gestation period was conducted in October 2012 at the Grenoble University Hospital Centre. A first culture-based GBS screening test was performed between 34 and 38 weeks of gestation followed by a second screening test at the onset of labor, using a real-time PCR Assay and a culture-based method (gold standard) in order to calculate the diagnostic accuracy.
RESULTS: One hundred an fifty-seven patients were enrolled. The sensitivity was 94.4% (95% CI, 72.7-99.9%) with intrapartum PCR assay and 50% (95% CI, 26-74%) with antepartum culture. Prevalence of GBS colonization was 7.6% with the antepartum culture method, 11.5% with intrapartum culture and 16.6% by using PCR-test.
CONCLUSION: Intrapartum PCR shows a much higher sensitivity compared to the antepartum culture-based screening mainly due to variations in GBS colonization and could allow us to target patients requiring intrapartum antibiotic prophylaxis more effectively.
| Medienart: |
E-Artikel |
|---|
| Erscheinungsjahr: |
2016 |
|---|---|
| Erschienen: |
2016 |
| Enthalten in: |
Zur Gesamtaufnahme - volume:45 |
|---|---|
| Enthalten in: |
Journal de gynecologie, obstetrique et biologie de la reproduction - 45(2016), 9 vom: 05. Nov., Seite 1151-1158 |
| Sprache: |
Französisch |
|---|
| Weiterer Titel: |
Intérêt d’un test de PCR en temps réel en intrapartum en comparaison à la culture de fin de grossesse pour le dépistage du streptocoque du groupe B chez la femme enceinte |
|---|
| Beteiligte Personen: |
Defez, M [VerfasserIn] |
|---|
| Links: |
|---|
| Themen: |
Antenatal screening test |
|---|
| Anmerkungen: |
Date Completed 05.06.2017 Date Revised 05.06.2017 published: Print-Electronic Citation Status MEDLINE |
|---|
| doi: |
10.1016/j.jgyn.2016.06.002 |
|---|
| funding: |
|
|---|---|
| Förderinstitution / Projekttitel: |
|
| PPN (Katalog-ID): |
NLM262063425 |
|---|
| LEADER | 01000naa a22002652 4500 | ||
|---|---|---|---|
| 001 | NLM262063425 | ||
| 003 | DE-627 | ||
| 005 | 20231224200934.0 | ||
| 007 | cr uuu---uuuuu | ||
| 008 | 231224s2016 xx |||||o 00| ||fre c | ||
| 024 | 7 | |a 10.1016/j.jgyn.2016.06.002 |2 doi | |
| 028 | 5 | 2 | |a pubmed24n0873.xml |
| 035 | |a (DE-627)NLM262063425 | ||
| 035 | |a (NLM)27378534 | ||
| 035 | |a (PII)S0368-2315(16)30057-6 | ||
| 040 | |a DE-627 |b ger |c DE-627 |e rakwb | ||
| 041 | |a fre | ||
| 100 | 1 | |a Defez, M |e verfasserin |4 aut | |
| 245 | 1 | 0 | |a Usefulness of a rapid intrapartum real-time PCR assay in comparison with the group B Streptococcus culture screening at the end of pregnancy in pregnant women |
| 246 | 3 | 3 | |a Intérêt d’un test de PCR en temps réel en intrapartum en comparaison à la culture de fin de grossesse pour le dépistage du streptocoque du groupe B chez la femme enceinte |
| 264 | 1 | |c 2016 | |
| 336 | |a Text |b txt |2 rdacontent | ||
| 337 | |a ƒaComputermedien |b c |2 rdamedia | ||
| 338 | |a ƒa Online-Ressource |b cr |2 rdacarrier | ||
| 500 | |a Date Completed 05.06.2017 | ||
| 500 | |a Date Revised 05.06.2017 | ||
| 500 | |a published: Print-Electronic | ||
| 500 | |a Citation Status MEDLINE | ||
| 520 | |a Copyright © 2016 Elsevier Masson SAS. All rights reserved. | ||
| 520 | |a OBJECTIVES: The objectives were to evaluate and compare the diagnostic accuracy of a rapid real-time PCR assay at the onset of labor with those of the current antenatal culture-based test at 34-38 weeks gestation for group B Streptococcus (GBS) screening | ||
| 520 | |a MATERIALS AND METHODS: A prospective study including all pregnant women admitted for delivery after a 34-week gestation period was conducted in October 2012 at the Grenoble University Hospital Centre. A first culture-based GBS screening test was performed between 34 and 38 weeks of gestation followed by a second screening test at the onset of labor, using a real-time PCR Assay and a culture-based method (gold standard) in order to calculate the diagnostic accuracy | ||
| 520 | |a RESULTS: One hundred an fifty-seven patients were enrolled. The sensitivity was 94.4% (95% CI, 72.7-99.9%) with intrapartum PCR assay and 50% (95% CI, 26-74%) with antepartum culture. Prevalence of GBS colonization was 7.6% with the antepartum culture method, 11.5% with intrapartum culture and 16.6% by using PCR-test | ||
| 520 | |a CONCLUSION: Intrapartum PCR shows a much higher sensitivity compared to the antepartum culture-based screening mainly due to variations in GBS colonization and could allow us to target patients requiring intrapartum antibiotic prophylaxis more effectively | ||
| 650 | 4 | |a Journal Article | |
| 650 | 4 | |a Antenatal screening test | |
| 650 | 4 | |a Dépistage anténatal | |
| 650 | 4 | |a Grossesse | |
| 650 | 4 | |a Group B Streptococcus | |
| 650 | 4 | |a PCR en temps réel | |
| 650 | 4 | |a Pregnancy | |
| 650 | 4 | |a Real-time polymerase chain reaction | |
| 650 | 4 | |a Streptocoque du groupe B | |
| 700 | 1 | |a Khizar, F |e verfasserin |4 aut | |
| 700 | 1 | |a Maurin, M |e verfasserin |4 aut | |
| 700 | 1 | |a Biot, F |e verfasserin |4 aut | |
| 700 | 1 | |a Pons, J-C |e verfasserin |4 aut | |
| 700 | 1 | |a Sergent, F |e verfasserin |4 aut | |
| 773 | 0 | 8 | |i Enthalten in |t Journal de gynecologie, obstetrique et biologie de la reproduction |d 1972 |g 45(2016), 9 vom: 05. Nov., Seite 1151-1158 |w (DE-627)NLM000005746 |x 1773-0430 |7 nnns |
| 773 | 1 | 8 | |g volume:45 |g year:2016 |g number:9 |g day:05 |g month:11 |g pages:1151-1158 |
| 856 | 4 | 0 | |u http://dx.doi.org/10.1016/j.jgyn.2016.06.002 |3 Volltext |
| 912 | |a GBV_USEFLAG_A | ||
| 912 | |a GBV_NLM | ||
| 951 | |a AR | ||
| 952 | |d 45 |j 2016 |e 9 |b 05 |c 11 |h 1151-1158 | ||