Epigenetic repression of ribosomal RNA transcription by ROCK-dependent aberrant cytoskeletal organization
It is known that ribosomal RNA (rRNA) synthesis is regulated by cellular energy and proliferation status. In this study, we investigated rRNA gene transcription in response to cytoskeletal stress. Our data revealed that the cell shape constrained by isotropic but not elongated micropatterns in HeLa cells led to a significant reduction in rRNA transcription dependent on ROCK. Expression of a dominant-active form of ROCK also repressed rRNA transcription. Isotropic constraint and ROCK over-activation led to different types of aberrant F-actin organization, but their suppression effects on rRNA transcription were similarly reversed by inhibition of histone deacetylase (HDAC) or overexpression of a dominant negative form of Nesprin, which shields the signal transmitted from actin filament to the nuclear interior. We further showed that the binding of HDAC1 to the active fraction of rDNA genes is increased by ROCK over-activation, thus reducing H3K9/14 acetylation and suppressing transcription. Our results demonstrate an epigenetic control of active rDNA genes that represses rRNA transcription in response to the cytoskeletal stress.
| Medienart: |
E-Artikel |
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| Erscheinungsjahr: |
2016 |
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| Erschienen: |
2016 |
| Enthalten in: |
Zur Gesamtaufnahme - volume:6 |
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| Enthalten in: |
Scientific reports - 6(2016) vom: 28. Juni, Seite 28685 |
| Sprache: |
Englisch |
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| Beteiligte Personen: |
Wu, Tse-Hsiang [VerfasserIn] |
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| Links: |
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| Themen: |
DNA, Ribosomal |
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| Anmerkungen: |
Date Completed 10.05.2018 Date Revised 13.11.2018 published: Electronic Citation Status MEDLINE |
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| doi: |
10.1038/srep28685 |
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| funding: |
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| Förderinstitution / Projekttitel: |
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| PPN (Katalog-ID): |
NLM261816608 |
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| 245 | 1 | 0 | |a Epigenetic repression of ribosomal RNA transcription by ROCK-dependent aberrant cytoskeletal organization |
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| 500 | |a Citation Status MEDLINE | ||
| 520 | |a It is known that ribosomal RNA (rRNA) synthesis is regulated by cellular energy and proliferation status. In this study, we investigated rRNA gene transcription in response to cytoskeletal stress. Our data revealed that the cell shape constrained by isotropic but not elongated micropatterns in HeLa cells led to a significant reduction in rRNA transcription dependent on ROCK. Expression of a dominant-active form of ROCK also repressed rRNA transcription. Isotropic constraint and ROCK over-activation led to different types of aberrant F-actin organization, but their suppression effects on rRNA transcription were similarly reversed by inhibition of histone deacetylase (HDAC) or overexpression of a dominant negative form of Nesprin, which shields the signal transmitted from actin filament to the nuclear interior. We further showed that the binding of HDAC1 to the active fraction of rDNA genes is increased by ROCK over-activation, thus reducing H3K9/14 acetylation and suppressing transcription. Our results demonstrate an epigenetic control of active rDNA genes that represses rRNA transcription in response to the cytoskeletal stress | ||
| 650 | 4 | |a Journal Article | |
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| 700 | 1 | |a Lee, Hsiao-Hui |e verfasserin |4 aut | |
| 700 | 1 | |a Kuo, Jean-Cheng |e verfasserin |4 aut | |
| 700 | 1 | |a Ou, Meng-Hsin |e verfasserin |4 aut | |
| 700 | 1 | |a Chang, Zee-Fen |e verfasserin |4 aut | |
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