Transcriptional signatures of influenza A/H1N1-specific IgG memory-like B cell response in older individuals
Copyright © 2016 Elsevier Ltd. All rights reserved..
BACKGROUND: Studies suggest that the recall-based humoral immune responses to influenza A/H1N1 originates from activated memory B cells. The aim of this study was to identify baseline, early and late blood transcriptional signatures (in peripheral blood mononuclear cells/PBMCs) associated with memory B cell response following influenza vaccination.
METHODS: We used pre- and post-vaccination mRNA-Seq transcriptional profiling on samples from 159 subjects (50-74years old) following receipt of seasonal trivalent influenza vaccine containing the A/California/7/2009/H1N1-like virus, and penalized regression modeling to identify associations with influenza A/H1N1-specific memory B cell ELISPOT response after vaccination.
RESULTS: Genesets and genes (p-value range 7.92E(-08) to 0.00018, q-value range 0.00019-0.039) demonstrating significant associations (of gene expression levels) with memory B cell response suggest the importance of metabolic (cholesterol and lipid metabolism-related), cell migration/adhesion, MAP kinase, NF-kB cell signaling (chemokine/cytokine signaling) and transcriptional regulation gene signatures in the development of memory B cell response after influenza vaccination.
CONCLUSION: Through an unbiased transcriptome-wide profiling approach, our study identified signatures of memory B cell response following influenza vaccination, highlighting the underappreciated role of metabolic changes (among the other immune function-related events) in the regulation of influenza vaccine-induced immune memory.
| Medienart: |
E-Artikel |
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| Erscheinungsjahr: |
2016 |
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| Erschienen: |
2016 |
| Enthalten in: |
Zur Gesamtaufnahme - volume:34 |
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| Enthalten in: |
Vaccine - 34(2016), 34 vom: 25. Juli, Seite 3993-4002 |
| Sprache: |
Englisch |
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| Beteiligte Personen: |
Haralambieva, Iana H [VerfasserIn] |
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| Links: |
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| Anmerkungen: |
Date Completed 07.11.2017 Date Revised 09.04.2022 published: Print-Electronic Citation Status MEDLINE |
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| doi: |
10.1016/j.vaccine.2016.06.034 |
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| funding: |
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| Förderinstitution / Projekttitel: |
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| PPN (Katalog-ID): |
NLM26152609X |
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| 100 | 1 | |a Haralambieva, Iana H |e verfasserin |4 aut | |
| 245 | 1 | 0 | |a Transcriptional signatures of influenza A/H1N1-specific IgG memory-like B cell response in older individuals |
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| 500 | |a Date Revised 09.04.2022 | ||
| 500 | |a published: Print-Electronic | ||
| 500 | |a Citation Status MEDLINE | ||
| 520 | |a Copyright © 2016 Elsevier Ltd. All rights reserved. | ||
| 520 | |a BACKGROUND: Studies suggest that the recall-based humoral immune responses to influenza A/H1N1 originates from activated memory B cells. The aim of this study was to identify baseline, early and late blood transcriptional signatures (in peripheral blood mononuclear cells/PBMCs) associated with memory B cell response following influenza vaccination | ||
| 520 | |a METHODS: We used pre- and post-vaccination mRNA-Seq transcriptional profiling on samples from 159 subjects (50-74years old) following receipt of seasonal trivalent influenza vaccine containing the A/California/7/2009/H1N1-like virus, and penalized regression modeling to identify associations with influenza A/H1N1-specific memory B cell ELISPOT response after vaccination | ||
| 520 | |a RESULTS: Genesets and genes (p-value range 7.92E(-08) to 0.00018, q-value range 0.00019-0.039) demonstrating significant associations (of gene expression levels) with memory B cell response suggest the importance of metabolic (cholesterol and lipid metabolism-related), cell migration/adhesion, MAP kinase, NF-kB cell signaling (chemokine/cytokine signaling) and transcriptional regulation gene signatures in the development of memory B cell response after influenza vaccination | ||
| 520 | |a CONCLUSION: Through an unbiased transcriptome-wide profiling approach, our study identified signatures of memory B cell response following influenza vaccination, highlighting the underappreciated role of metabolic changes (among the other immune function-related events) in the regulation of influenza vaccine-induced immune memory | ||
| 650 | 4 | |a Journal Article | |
| 650 | 4 | |a Research Support, N.I.H., Extramural | |
| 650 | 4 | |a Aging | |
| 650 | 4 | |a B-lymphocytes | |
| 650 | 4 | |a Enzyme-linked immunospot assay | |
| 650 | 4 | |a Gene expression | |
| 650 | 4 | |a Immunity, Humoral | |
| 650 | 4 | |a Influenza A virus, H1N1 subtype | |
| 650 | 4 | |a Influenza vaccines | |
| 650 | 4 | |a Influenza, Human | |
| 650 | 4 | |a RNA | |
| 650 | 4 | |a Sequence analysis | |
| 650 | 4 | |a Vaccination | |
| 650 | 7 | |a Antibodies, Viral |2 NLM | |
| 650 | 7 | |a Influenza Vaccines |2 NLM | |
| 700 | 1 | |a Ovsyannikova, Inna G |e verfasserin |4 aut | |
| 700 | 1 | |a Kennedy, Richard B |e verfasserin |4 aut | |
| 700 | 1 | |a Zimmermann, Michael T |e verfasserin |4 aut | |
| 700 | 1 | |a Grill, Diane E |e verfasserin |4 aut | |
| 700 | 1 | |a Oberg, Ann L |e verfasserin |4 aut | |
| 700 | 1 | |a Poland, Gregory A |e verfasserin |4 aut | |
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