Gonadotropin-releasing hormone analogues lead to pro-inflammatory changes in T lymphocytes
© 2016 John Wiley & Sons A/S. Published by John Wiley & Sons Ltd..
PROBLEM: We aimed to investigate the effect of gonadotropin-releasing hormone (GnRH) analogues on T-cell immunity.
METHOD OF STUDY: TNF-α(+) -, INF-ɣ(+) -, IL-10(+) -, and IL-17(+) -expressing T cells in peripheral blood mononuclear cells (PBMCs) were treated with various concentrations (0.1, 1, 5, and 10 μm) of GnRH agonist (buserelin acetate) and antagonist (cetrorelix acetate) for 4 hours in vitro and they were analyzed with flow cytometry.
RESULTS: TNF-α(+) /IL-10(+) T helper (TH) cell ratios were increased in PBMCs treated with 1, 5, and 10 μm GnRH agonist when compared to controls (P=.006, P=.014 and P=.030, respectively). IFN-ɣ(+) /IL-10(+) TH cell ratios were significantly increased with 0.1, 1, 5, and 10 μm GnRH agonist as compared with controls (P=.046, P=.004, P=.013, and P=.011, respectively). TNF-α(+) TH cell levels, and IFN-γ(+) /IL-10(+) TH cell ratios were significantly different (P<.001 and P<.004, respectively) between GnRH agonist- and antagonist-treated cells.
CONCLUSION: GnRH analogues induce pro-inflammatory TH1 shift in T-cell immunity, in vitro. GnRH treatment during assisted reproductive technology cycle might explain a possible cause of inflammatory flare in women with inflammatory conditions.
| Medienart: |
E-Artikel |
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| Erscheinungsjahr: |
2016 |
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| Erschienen: |
2016 |
| Enthalten in: |
Zur Gesamtaufnahme - volume:76 |
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| Enthalten in: |
American journal of reproductive immunology (New York, N.Y. : 1989) - 76(2016), 1 vom: 13. Juli, Seite 50-8 |
| Sprache: |
Englisch |
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| Beteiligte Personen: |
Sung, Nayoung [VerfasserIn] |
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| Links: |
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| Anmerkungen: |
Date Completed 13.12.2017 Date Revised 03.04.2018 published: Print Citation Status MEDLINE |
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| doi: |
10.1111/aji.12522 |
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| funding: |
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| Förderinstitution / Projekttitel: |
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NLM261316842 |
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| 500 | |a published: Print | ||
| 500 | |a Citation Status MEDLINE | ||
| 520 | |a © 2016 John Wiley & Sons A/S. Published by John Wiley & Sons Ltd. | ||
| 520 | |a PROBLEM: We aimed to investigate the effect of gonadotropin-releasing hormone (GnRH) analogues on T-cell immunity | ||
| 520 | |a METHOD OF STUDY: TNF-α(+) -, INF-ɣ(+) -, IL-10(+) -, and IL-17(+) -expressing T cells in peripheral blood mononuclear cells (PBMCs) were treated with various concentrations (0.1, 1, 5, and 10 μm) of GnRH agonist (buserelin acetate) and antagonist (cetrorelix acetate) for 4 hours in vitro and they were analyzed with flow cytometry | ||
| 520 | |a RESULTS: TNF-α(+) /IL-10(+) T helper (TH) cell ratios were increased in PBMCs treated with 1, 5, and 10 μm GnRH agonist when compared to controls (P=.006, P=.014 and P=.030, respectively). IFN-ɣ(+) /IL-10(+) TH cell ratios were significantly increased with 0.1, 1, 5, and 10 μm GnRH agonist as compared with controls (P=.046, P=.004, P=.013, and P=.011, respectively). TNF-α(+) TH cell levels, and IFN-γ(+) /IL-10(+) TH cell ratios were significantly different (P<.001 and P<.004, respectively) between GnRH agonist- and antagonist-treated cells | ||
| 520 | |a CONCLUSION: GnRH analogues induce pro-inflammatory TH1 shift in T-cell immunity, in vitro. GnRH treatment during assisted reproductive technology cycle might explain a possible cause of inflammatory flare in women with inflammatory conditions | ||
| 650 | 4 | |a Journal Article | |
| 650 | 4 | |a Research Support, Non-U.S. Gov't | |
| 650 | 4 | |a GnRH agonist | |
| 650 | 4 | |a GnRH antagonist | |
| 650 | 4 | |a T helper (TH) 1 immunity | |
| 650 | 4 | |a TH17 | |
| 650 | 4 | |a gonadotropin-releasing hormone (GnRH) | |
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| 700 | 1 | |a Dambaeva, Svetlana |e verfasserin |4 aut | |
| 700 | 1 | |a Beaman, Kenneth D |e verfasserin |4 aut | |
| 700 | 1 | |a Gilman-Sachs, Alice |e verfasserin |4 aut | |
| 700 | 1 | |a Kwak-Kim, Joanne |e verfasserin |4 aut | |
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