Inhibition of mitochondrial calcium uniporter protects neurocytes from ischemia/reperfusion injury via the inhibition of excessive mitophagy
Copyright © 2016 Elsevier Ireland Ltd. All rights reserved..
Mitophagy plays an important role in mitochondrial quality control and cell survival during the process of ischemia/reperfusion (I/R) injury. Mitochondrial calcium uniporter (MCU) is the most important channel responsible for Ca(2+) influx into mitochondria and Ca(2+) signal plays a potential role in modulating mitophagy. However, the effect of MCU on mitophagy during the process of I/R injury remains unknown. This study constructed an in vitro I/R model by subjecting oxygen and glucose deprivation/reperfusion (OGD/RP) model to SH-SY5Y cells to mimic the cerebral I/R injury and aimed to explore the exact effect of MCU on I/R induced mitophagy. The results showed that OGD/RP induced autophagy and mitophagy in SH-SY5Y cells. Ru360, the inhibitor of MCU, improved mitochondrial morphology and fuctional stability as well as cell viability, significantly reduced OGD/RP induced mitophagy as evidenced by the decrease in Beclin-1 and the increase in Tom20 and P62 expression. Whereas spermine, the agonist of MCU, had no significant impact on the expression of those mitophagy related proteins compared with OGD/RP group. This study indicates that inhibition of MCU can inhibit excessive mitophagy and protect the neurocytes from I/R injury.
| Medienart: |
E-Artikel |
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| Erscheinungsjahr: |
2016 |
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| Erschienen: |
2016 |
| Enthalten in: |
Zur Gesamtaufnahme - volume:628 |
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| Enthalten in: |
Neuroscience letters - 628(2016) vom: 15. Aug., Seite 24-9 |
| Sprache: |
Englisch |
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| Beteiligte Personen: |
Yu, Shoushui [VerfasserIn] |
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| Links: |
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| Anmerkungen: |
Date Completed 13.06.2017 Date Revised 04.12.2021 published: Print-Electronic Citation Status MEDLINE |
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| doi: |
10.1016/j.neulet.2016.06.012 |
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| funding: |
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| Förderinstitution / Projekttitel: |
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| 100 | 1 | |a Yu, Shoushui |e verfasserin |4 aut | |
| 245 | 1 | 0 | |a Inhibition of mitochondrial calcium uniporter protects neurocytes from ischemia/reperfusion injury via the inhibition of excessive mitophagy |
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| 500 | |a Date Revised 04.12.2021 | ||
| 500 | |a published: Print-Electronic | ||
| 500 | |a Citation Status MEDLINE | ||
| 520 | |a Copyright © 2016 Elsevier Ireland Ltd. All rights reserved. | ||
| 520 | |a Mitophagy plays an important role in mitochondrial quality control and cell survival during the process of ischemia/reperfusion (I/R) injury. Mitochondrial calcium uniporter (MCU) is the most important channel responsible for Ca(2+) influx into mitochondria and Ca(2+) signal plays a potential role in modulating mitophagy. However, the effect of MCU on mitophagy during the process of I/R injury remains unknown. This study constructed an in vitro I/R model by subjecting oxygen and glucose deprivation/reperfusion (OGD/RP) model to SH-SY5Y cells to mimic the cerebral I/R injury and aimed to explore the exact effect of MCU on I/R induced mitophagy. The results showed that OGD/RP induced autophagy and mitophagy in SH-SY5Y cells. Ru360, the inhibitor of MCU, improved mitochondrial morphology and fuctional stability as well as cell viability, significantly reduced OGD/RP induced mitophagy as evidenced by the decrease in Beclin-1 and the increase in Tom20 and P62 expression. Whereas spermine, the agonist of MCU, had no significant impact on the expression of those mitophagy related proteins compared with OGD/RP group. This study indicates that inhibition of MCU can inhibit excessive mitophagy and protect the neurocytes from I/R injury | ||
| 650 | 4 | |a Journal Article | |
| 650 | 4 | |a Ischemia/reperfusion injury | |
| 650 | 4 | |a MCU | |
| 650 | 4 | |a Mitophagy | |
| 650 | 4 | |a Neuroprotection | |
| 650 | 7 | |a BECN1 protein, human |2 NLM | |
| 650 | 7 | |a Beclin-1 |2 NLM | |
| 650 | 7 | |a Calcium Channels |2 NLM | |
| 650 | 7 | |a Membrane Transport Proteins |2 NLM | |
| 650 | 7 | |a Mitochondrial Precursor Protein Import Complex Proteins |2 NLM | |
| 650 | 7 | |a P62 protein, human |2 NLM | |
| 650 | 7 | |a RNA-Binding Proteins |2 NLM | |
| 650 | 7 | |a Receptors, Cell Surface |2 NLM | |
| 650 | 7 | |a Ru 360 |2 NLM | |
| 650 | 7 | |a Ruthenium Compounds |2 NLM | |
| 650 | 7 | |a TOMM20 protein, human |2 NLM | |
| 650 | 7 | |a mitochondrial calcium uniporter |2 NLM | |
| 650 | 7 | |a Spermine |2 NLM | |
| 650 | 7 | |a 2FZ7Y3VOQX |2 NLM | |
| 700 | 1 | |a Zheng, Shengfa |e verfasserin |4 aut | |
| 700 | 1 | |a Leng, Jing |e verfasserin |4 aut | |
| 700 | 1 | |a Wang, Shilei |e verfasserin |4 aut | |
| 700 | 1 | |a Zhao, Tao |e verfasserin |4 aut | |
| 700 | 1 | |a Liu, Jia |e verfasserin |4 aut | |
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| 856 | 4 | 0 | |u http://dx.doi.org/10.1016/j.neulet.2016.06.012 |3 Volltext |
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