The CENP-T/-W complex is a binding partner of the histone chaperone FACT
© 2016 Prendergast et al.; Published by Cold Spring Harbor Laboratory Press..
The CENP-T/-W histone fold complex, as an integral part of the inner kinetochore, is essential for building a proper kinetochore at the centromere in order to direct chromosome segregation during mitosis. Notably, CENP-T/-W is not inherited at centromeres, and new deposition is absolutely required at each cell cycle for kinetochore function. However, the mechanisms underlying this new deposition of CENP-T/-W at centromeres are unclear. Here, we found that CENP-T deposition at centromeres is uncoupled from DNA synthesis. We identified Spt16 and SSRP1, subunits of the H2A-H2B histone chaperone facilitates chromatin transcription (FACT), as CENP-W binding partners through a proteomic screen. We found that the C-terminal region of Spt16 binds specifically to the histone fold region of CENP-T/-W. Furthermore, depletion of Spt16 impairs CENP-T and CENP-W deposition at endogenous centromeres, and site-directed targeting of Spt16 alone is sufficient to ensure local de novo CENP-T accumulation. We propose a model in which the FACT chaperone stabilizes the soluble CENP-T/-W complex in the cell and promotes dynamics of exchange, enabling CENP-T/-W deposition at centromeres.
| Errataetall: |
CommentIn: Trends Biochem Sci. 2016 Sep;41(9):736-8. - PMID 27499233 |
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| Medienart: |
E-Artikel |
| Erscheinungsjahr: |
2016 |
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| Erschienen: |
2016 |
| Enthalten in: |
Zur Gesamtaufnahme - volume:30 |
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| Enthalten in: |
Genes & development - 30(2016), 11 vom: 01. Juni, Seite 1313-26 |
| Sprache: |
Englisch |
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| Beteiligte Personen: |
Prendergast, Lisa [VerfasserIn] |
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| Links: |
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| Anmerkungen: |
Date Completed 27.04.2017 Date Revised 13.11.2018 published: Print-Electronic CommentIn: Trends Biochem Sci. 2016 Sep;41(9):736-8. - PMID 27499233 Citation Status MEDLINE |
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| doi: |
10.1101/gad.275073.115 |
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| funding: |
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| Förderinstitution / Projekttitel: |
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| PPN (Katalog-ID): |
NLM261255460 |
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| 100 | 1 | |a Prendergast, Lisa |e verfasserin |4 aut | |
| 245 | 1 | 4 | |a The CENP-T/-W complex is a binding partner of the histone chaperone FACT |
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| 500 | |a CommentIn: Trends Biochem Sci. 2016 Sep;41(9):736-8. - PMID 27499233 | ||
| 500 | |a Citation Status MEDLINE | ||
| 520 | |a © 2016 Prendergast et al.; Published by Cold Spring Harbor Laboratory Press. | ||
| 520 | |a The CENP-T/-W histone fold complex, as an integral part of the inner kinetochore, is essential for building a proper kinetochore at the centromere in order to direct chromosome segregation during mitosis. Notably, CENP-T/-W is not inherited at centromeres, and new deposition is absolutely required at each cell cycle for kinetochore function. However, the mechanisms underlying this new deposition of CENP-T/-W at centromeres are unclear. Here, we found that CENP-T deposition at centromeres is uncoupled from DNA synthesis. We identified Spt16 and SSRP1, subunits of the H2A-H2B histone chaperone facilitates chromatin transcription (FACT), as CENP-W binding partners through a proteomic screen. We found that the C-terminal region of Spt16 binds specifically to the histone fold region of CENP-T/-W. Furthermore, depletion of Spt16 impairs CENP-T and CENP-W deposition at endogenous centromeres, and site-directed targeting of Spt16 alone is sufficient to ensure local de novo CENP-T accumulation. We propose a model in which the FACT chaperone stabilizes the soluble CENP-T/-W complex in the cell and promotes dynamics of exchange, enabling CENP-T/-W deposition at centromeres | ||
| 650 | 4 | |a Journal Article | |
| 650 | 4 | |a Research Support, Non-U.S. Gov't | |
| 650 | 4 | |a CENP | |
| 650 | 4 | |a centromere | |
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| 650 | 7 | |a CENPT protein, human |2 NLM | |
| 650 | 7 | |a CENPW protein, human |2 NLM | |
| 650 | 7 | |a Cell Cycle Proteins |2 NLM | |
| 650 | 7 | |a Chromosomal Proteins, Non-Histone |2 NLM | |
| 650 | 7 | |a DNA-Binding Proteins |2 NLM | |
| 650 | 7 | |a High Mobility Group Proteins |2 NLM | |
| 650 | 7 | |a Histone Chaperones |2 NLM | |
| 650 | 7 | |a Recombinant Proteins |2 NLM | |
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| 650 | 7 | |a SUPT16H protein, human |2 NLM | |
| 650 | 7 | |a Transcription Factors |2 NLM | |
| 650 | 7 | |a Transcriptional Elongation Factors |2 NLM | |
| 700 | 1 | |a Müller, Sebastian |e verfasserin |4 aut | |
| 700 | 1 | |a Liu, Yiwei |e verfasserin |4 aut | |
| 700 | 1 | |a Huang, Hongda |e verfasserin |4 aut | |
| 700 | 1 | |a Dingli, Florent |e verfasserin |4 aut | |
| 700 | 1 | |a Loew, Damarys |e verfasserin |4 aut | |
| 700 | 1 | |a Vassias, Isabelle |e verfasserin |4 aut | |
| 700 | 1 | |a Patel, Dinshaw J |e verfasserin |4 aut | |
| 700 | 1 | |a Sullivan, Kevin F |e verfasserin |4 aut | |
| 700 | 1 | |a Almouzni, Geneviève |e verfasserin |4 aut | |
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