Biphasic effects of l-ascorbate on the tumoricidal activity of non-thermal plasma against malignant mesothelioma cells
Copyright © 2016 Elsevier Inc. All rights reserved..
Non-thermal plasma (NTP) is a recently developed technology that elicits a variety of biological effects. This includes cancer cell-specific cytotoxicity, which is mainly attributed to the regional generation of reactive oxygen species (ROS). We studied the effects of NTP on malignant mesothelioma (MM) and its modulation by l-ascorbate. l-ascorbate is a major water-soluble anti-oxidant in vivo, but its pro-oxidant activity in vitro has been well recognized. Thus, the effects of ascorbate on the efficacy of NTP is important to examine. NTP exposure dose-dependently killed MM cells, whereas MM cells tolerated 1 mM l-ascorbate. However, brief pre-treatment with a pharmacological dose (250-750 μM) of l-ascorbate immediately prior to NTP exposure significantly increased its cytotoxicity in a dose-dependent manner, which was inhibited by the iron chelator, deferoxamine. However, paradoxically, this potentiating effect of l-ascorbate was completely abolished by a prolonged 4 h pre-incubation with l-ascorbate (500 μM). MM cytotoxicity induced by NTP was associated with immediate oxidative stress evaluated by 2',7'-dichlorodihydrofluorecein diacetate, which was followed by an increase in the expression of the autophagosome marker, LC3B-II. In conclusion, MM can be a target for NTP treatment and l-ascorbate can increase or decrease its efficacy depending on the length of the pre-incubation period.
| Medienart: |
E-Artikel |
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| Erscheinungsjahr: |
2016 |
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| Erschienen: |
2016 |
| Enthalten in: |
Zur Gesamtaufnahme - volume:605 |
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| Enthalten in: |
Archives of biochemistry and biophysics - 605(2016) vom: 01. Sept., Seite 109-16 |
| Sprache: |
Englisch |
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| Beteiligte Personen: |
Shi, Lei [VerfasserIn] |
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| Links: |
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| Anmerkungen: |
Date Completed 02.05.2017 Date Revised 18.12.2020 published: Print-Electronic Citation Status MEDLINE |
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| doi: |
10.1016/j.abb.2016.05.016 |
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| funding: |
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| Förderinstitution / Projekttitel: |
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| 520 | |a Non-thermal plasma (NTP) is a recently developed technology that elicits a variety of biological effects. This includes cancer cell-specific cytotoxicity, which is mainly attributed to the regional generation of reactive oxygen species (ROS). We studied the effects of NTP on malignant mesothelioma (MM) and its modulation by l-ascorbate. l-ascorbate is a major water-soluble anti-oxidant in vivo, but its pro-oxidant activity in vitro has been well recognized. Thus, the effects of ascorbate on the efficacy of NTP is important to examine. NTP exposure dose-dependently killed MM cells, whereas MM cells tolerated 1 mM l-ascorbate. However, brief pre-treatment with a pharmacological dose (250-750 μM) of l-ascorbate immediately prior to NTP exposure significantly increased its cytotoxicity in a dose-dependent manner, which was inhibited by the iron chelator, deferoxamine. However, paradoxically, this potentiating effect of l-ascorbate was completely abolished by a prolonged 4 h pre-incubation with l-ascorbate (500 μM). MM cytotoxicity induced by NTP was associated with immediate oxidative stress evaluated by 2',7'-dichlorodihydrofluorecein diacetate, which was followed by an increase in the expression of the autophagosome marker, LC3B-II. In conclusion, MM can be a target for NTP treatment and l-ascorbate can increase or decrease its efficacy depending on the length of the pre-incubation period | ||
| 650 | 4 | |a Journal Article | |
| 650 | 4 | |a Autophagy | |
| 650 | 4 | |a Mesothelioma | |
| 650 | 4 | |a Non-thermal plasma (NTP) | |
| 650 | 4 | |a Reactive oxygen species (ROS) | |
| 650 | 4 | |a l-ascorbate | |
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| 650 | 7 | |a Antioxidants |2 NLM | |
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| 700 | 1 | |a Wang, Yue |e verfasserin |4 aut | |
| 700 | 1 | |a Ito, Fumiya |e verfasserin |4 aut | |
| 700 | 1 | |a Okazaki, Yasumasa |e verfasserin |4 aut | |
| 700 | 1 | |a Tanaka, Hiromasa |e verfasserin |4 aut | |
| 700 | 1 | |a Mizuno, Masaaki |e verfasserin |4 aut | |
| 700 | 1 | |a Hori, Masaru |e verfasserin |4 aut | |
| 700 | 1 | |a Richardson, Des R |e verfasserin |4 aut | |
| 700 | 1 | |a Toyokuni, Shinya |e verfasserin |4 aut | |
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