Rapid Identification of Major Histocompatibility Complex Class I Haplotypes Using Deep Sequencing in an Endangered Old World Monkey
Immunogenetic data from wild primate populations have been difficult to obtain, due to logistic and methodological constraints. We applied a well-characterized deep sequencing method for MHC I typing, developed for macaques, to a population of wild red colobus to assess the feasibility of identifying MHC I-A/B haplotypes. Ten individuals produced sufficient data from blood and tissue samples to assign haplotypes. Eighty-two sequences were classified as red colobus MHC I alleles distributed across six MHC I loci. Individuals averaged ~13k reads across six MHC I loci, with 83% of all alleles representing either MHC I-A or MHC I-B loci. This study not only represents an important advance in the identification and genotyping of MHC in the endangered red colobus but also shows the potential for using this approach in other endangered wild primates.
| Medienart: |
Artikel |
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| Erscheinungsjahr: |
2016 |
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| Erschienen: |
2016 |
| Enthalten in: |
Zur Gesamtaufnahme - volume:8 |
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| Enthalten in: |
Conservation genetics resources - 8(2016), 1 vom: 01. März, Seite 23-26 |
| Sprache: |
Englisch |
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| Beteiligte Personen: |
Simons, Noah D [VerfasserIn] |
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| Themen: |
Immunogenetics |
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| Anmerkungen: |
Date Revised 01.10.2020 published: Print-Electronic Citation Status PubMed-not-MEDLINE |
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| Förderinstitution / Projekttitel: |
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| PPN (Katalog-ID): |
NLM260374520 |
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| 245 | 1 | 0 | |a Rapid Identification of Major Histocompatibility Complex Class I Haplotypes Using Deep Sequencing in an Endangered Old World Monkey |
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| 520 | |a Immunogenetic data from wild primate populations have been difficult to obtain, due to logistic and methodological constraints. We applied a well-characterized deep sequencing method for MHC I typing, developed for macaques, to a population of wild red colobus to assess the feasibility of identifying MHC I-A/B haplotypes. Ten individuals produced sufficient data from blood and tissue samples to assign haplotypes. Eighty-two sequences were classified as red colobus MHC I alleles distributed across six MHC I loci. Individuals averaged ~13k reads across six MHC I loci, with 83% of all alleles representing either MHC I-A or MHC I-B loci. This study not only represents an important advance in the identification and genotyping of MHC in the endangered red colobus but also shows the potential for using this approach in other endangered wild primates | ||
| 650 | 4 | |a Journal Article | |
| 650 | 4 | |a Major Histocompatibility Complex | |
| 650 | 4 | |a immunogenetics | |
| 650 | 4 | |a next generation sequencing | |
| 650 | 4 | |a primates | |
| 650 | 4 | |a red colobus | |
| 700 | 1 | |a Ruiz-Lopez, Maria Jose |e verfasserin |4 aut | |
| 700 | 1 | |a Chapman, Colin A |e verfasserin |4 aut | |
| 700 | 1 | |a Goldberg, Tony L |e verfasserin |4 aut | |
| 700 | 1 | |a Karl, Julie A |e verfasserin |4 aut | |
| 700 | 1 | |a Wiseman, Roger W |e verfasserin |4 aut | |
| 700 | 1 | |a Bohn, Patrick S |e verfasserin |4 aut | |
| 700 | 1 | |a O'Connor, David H |e verfasserin |4 aut | |
| 700 | 1 | |a Ting, Nelson |e verfasserin |4 aut | |
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