Molecular Epidemiology of Bartonella henselae in Stray and Sheltered Cats of Zaragoza, Spain
OBJECTIVE: Bartonella henselae is responsible for the Cat Scratch Disease in humans, being it underdiagnosed. This study aims to detect and quantify the load of B. henselae DNA in oral and whole blood samples from stray and shelthered cats from Zaragoza (Spain), and analyze associations with epidemiological and clinical factors.
METHODS: 47 cats entered in the estudy. Real time PCR was used to detect B. henselae DNA in blood and oral samples. The SPSS software was applied to the statistical analysis of positivity of paired samples and its relationship with variables as age, sex, origin, month of sampling and fleas/ticks observation in fur and clinical factors (health status and observation of oral lesions). To know the relationship between the presence in blood and oral cavity a logistic regression analysis was performed.
RESULTS: A 23.40% of blood samples and the 27.65% of the oral swabs carried the B. henselae DNA. A fair agreement between paired samples was observed (kappa value = 0.33, p less than 0.05). Bacterial DNA detected in oral and blood samples were not significantly associated to any of the epidemiological and clinical factors. Positive cats having oral lesions carried higher loads (3,12/1x1,000,000 cells) of bacterial DNA in their oral cavity than those without lesions (2,58/1x1,000,000 cells) being p=0.032.
CONCLUSIONS: Carriage of the B. henselae DNA in the blood samples appears not to be related with carriage of the DNA of the bacteria in mouth and vice versa. Positive cats having oral lesions carry a higher load of B. henselae DNA and may suppose a higher risk of transmission to people handling them.
| Medienart: |
E-Artikel |
|---|
| Erscheinungsjahr: |
2016 |
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| Erschienen: |
2016 |
| Enthalten in: |
Zur Gesamtaufnahme - volume:90 |
|---|---|
| Enthalten in: |
Revista espanola de salud publica - 90(2016) vom: 05. Mai, Seite E5 |
| Sprache: |
Spanisch |
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| Weiterer Titel: |
Epidemiología molecular de Bartonella henselae en gatos callejeros y de albergue en Zaragoza, España |
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| Beteiligte Personen: |
Alamán Valtierra, Manuel [VerfasserIn] |
|---|
| Themen: |
Bartonella Henselae |
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| Anmerkungen: |
Date Completed 13.07.2016 Date Revised 02.12.2018 published: Electronic Citation Status MEDLINE |
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| Förderinstitution / Projekttitel: |
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| PPN (Katalog-ID): |
NLM260031909 |
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| 100 | 1 | |a Alamán Valtierra, Manuel |e verfasserin |4 aut | |
| 245 | 1 | 0 | |a Molecular Epidemiology of Bartonella henselae in Stray and Sheltered Cats of Zaragoza, Spain |
| 246 | 3 | 3 | |a Epidemiología molecular de Bartonella henselae en gatos callejeros y de albergue en Zaragoza, España |
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| 500 | |a Date Completed 13.07.2016 | ||
| 500 | |a Date Revised 02.12.2018 | ||
| 500 | |a published: Electronic | ||
| 500 | |a Citation Status MEDLINE | ||
| 520 | |a OBJECTIVE: Bartonella henselae is responsible for the Cat Scratch Disease in humans, being it underdiagnosed. This study aims to detect and quantify the load of B. henselae DNA in oral and whole blood samples from stray and shelthered cats from Zaragoza (Spain), and analyze associations with epidemiological and clinical factors | ||
| 520 | |a METHODS: 47 cats entered in the estudy. Real time PCR was used to detect B. henselae DNA in blood and oral samples. The SPSS software was applied to the statistical analysis of positivity of paired samples and its relationship with variables as age, sex, origin, month of sampling and fleas/ticks observation in fur and clinical factors (health status and observation of oral lesions). To know the relationship between the presence in blood and oral cavity a logistic regression analysis was performed | ||
| 520 | |a RESULTS: A 23.40% of blood samples and the 27.65% of the oral swabs carried the B. henselae DNA. A fair agreement between paired samples was observed (kappa value = 0.33, p less than 0.05). Bacterial DNA detected in oral and blood samples were not significantly associated to any of the epidemiological and clinical factors. Positive cats having oral lesions carried higher loads (3,12/1x1,000,000 cells) of bacterial DNA in their oral cavity than those without lesions (2,58/1x1,000,000 cells) being p=0.032 | ||
| 520 | |a CONCLUSIONS: Carriage of the B. henselae DNA in the blood samples appears not to be related with carriage of the DNA of the bacteria in mouth and vice versa. Positive cats having oral lesions carry a higher load of B. henselae DNA and may suppose a higher risk of transmission to people handling them | ||
| 650 | 4 | |a Journal Article | |
| 650 | 4 | |a Bartonella Henselae | |
| 650 | 4 | |a Cats | |
| 650 | 4 | |a Communicable Diseases | |
| 650 | 4 | |a Logistic Regression | |
| 650 | 4 | |a Molecular epidemiology | |
| 650 | 4 | |a Pets | |
| 650 | 4 | |a Real-Time Polymerase Chain Reaction | |
| 650 | 4 | |a Spain | |
| 650 | 4 | |a Zoonoses | |
| 650 | 7 | |a DNA, Bacterial |2 NLM | |
| 700 | 1 | |a Simón Valencia, Carmen |e verfasserin |4 aut | |
| 700 | 1 | |a Fuertes Negro, Hector |e verfasserin |4 aut | |
| 700 | 1 | |a Unzueta Galarza, Amaya |e verfasserin |4 aut | |
| 700 | 1 | |a Flores Somarriba, Byron |e verfasserin |4 aut | |
| 700 | 1 | |a Halaihel Kassab, Nabil |e verfasserin |4 aut | |
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