1,8-Cineole potentiates IRF3-mediated antiviral response in human stem cells and in an ex vivo model of rhinosinusitis
© 2016 The Author(s). published by Portland Press Limited on behalf of the Biochemical Society..
The common cold is one of the most frequent human inflammatory diseases caused by viruses and can facilitate bacterial superinfections, resulting in sinusitis or pneumonia. The active ingredient of the drug Soledum, 1,8-cineole, is commonly applied for treating inflammatory diseases of the respiratory tract. However, the potential for 1,8-cineole to treat primary viral infections of the respiratory tract remains unclear. In the present study, we demonstrate for the first time that 1,8-cineole potentiates poly(I:C)-induced activity of the antiviral transcription factor interferon regulatory factor 3 (IRF3), while simultaneously reducing proinflammatory nuclear factor (NF)-κB activity in human cell lines, inferior turbinate stem cells (ITSCs) and in ex vivo cultivated human nasal mucosa. Co-treatment of cell lines with poly(I:C) and 1,8-cineole resulted in significantly increased IRF3 reporter gene activity compared with poly(I:C) alone, whereas NF-κB activity was reduced. Accordingly, 1,8-cineole- and poly(I:C) treatment led to increased nuclear translocation of IRF3 in ITSCs and a human ex vivo model of rhinosinusitis compared with the poly(I:C) treatment approach. Nuclear translocation of IRF3 was significantly increased in ITSCs and slice cultures treated with lipopolysaccharide (LPS) and 1,8-cineole compared with the LPS-treated cells mimicking bacterial infection. Our findings strongly suggest that 1,8-cineole potentiates the antiviral activity of IRF3 in addition to its inhibitory effect on proinflammatory NF-κB signalling, and may thus broaden its field of application.
| Medienart: |
E-Artikel |
|---|
| Erscheinungsjahr: |
2016 |
|---|---|
| Erschienen: |
2016 |
| Enthalten in: |
Zur Gesamtaufnahme - volume:130 |
|---|---|
| Enthalten in: |
Clinical science (London, England : 1979) - 130(2016), 15 vom: 01. Aug., Seite 1339-52 |
| Sprache: |
Englisch |
|---|
| Beteiligte Personen: |
Müller, Janine [VerfasserIn] |
|---|
| Links: |
|---|
| Anmerkungen: |
Date Completed 03.07.2017 Date Revised 03.12.2018 published: Print-Electronic Citation Status MEDLINE |
|---|
| doi: |
10.1042/CS20160218 |
|---|
| funding: |
|
|---|---|
| Förderinstitution / Projekttitel: |
|
| PPN (Katalog-ID): |
NLM259874124 |
|---|
| LEADER | 01000naa a22002652 4500 | ||
|---|---|---|---|
| 001 | NLM259874124 | ||
| 003 | DE-627 | ||
| 005 | 20231224192141.0 | ||
| 007 | cr uuu---uuuuu | ||
| 008 | 231224s2016 xx |||||o 00| ||eng c | ||
| 024 | 7 | |a 10.1042/CS20160218 |2 doi | |
| 028 | 5 | 2 | |a pubmed24n0866.xml |
| 035 | |a (DE-627)NLM259874124 | ||
| 035 | |a (NLM)27129189 | ||
| 040 | |a DE-627 |b ger |c DE-627 |e rakwb | ||
| 041 | |a eng | ||
| 100 | 1 | |a Müller, Janine |e verfasserin |4 aut | |
| 245 | 1 | 0 | |a 1,8-Cineole potentiates IRF3-mediated antiviral response in human stem cells and in an ex vivo model of rhinosinusitis |
| 264 | 1 | |c 2016 | |
| 336 | |a Text |b txt |2 rdacontent | ||
| 337 | |a ƒaComputermedien |b c |2 rdamedia | ||
| 338 | |a ƒa Online-Ressource |b cr |2 rdacarrier | ||
| 500 | |a Date Completed 03.07.2017 | ||
| 500 | |a Date Revised 03.12.2018 | ||
| 500 | |a published: Print-Electronic | ||
| 500 | |a Citation Status MEDLINE | ||
| 520 | |a © 2016 The Author(s). published by Portland Press Limited on behalf of the Biochemical Society. | ||
| 520 | |a The common cold is one of the most frequent human inflammatory diseases caused by viruses and can facilitate bacterial superinfections, resulting in sinusitis or pneumonia. The active ingredient of the drug Soledum, 1,8-cineole, is commonly applied for treating inflammatory diseases of the respiratory tract. However, the potential for 1,8-cineole to treat primary viral infections of the respiratory tract remains unclear. In the present study, we demonstrate for the first time that 1,8-cineole potentiates poly(I:C)-induced activity of the antiviral transcription factor interferon regulatory factor 3 (IRF3), while simultaneously reducing proinflammatory nuclear factor (NF)-κB activity in human cell lines, inferior turbinate stem cells (ITSCs) and in ex vivo cultivated human nasal mucosa. Co-treatment of cell lines with poly(I:C) and 1,8-cineole resulted in significantly increased IRF3 reporter gene activity compared with poly(I:C) alone, whereas NF-κB activity was reduced. Accordingly, 1,8-cineole- and poly(I:C) treatment led to increased nuclear translocation of IRF3 in ITSCs and a human ex vivo model of rhinosinusitis compared with the poly(I:C) treatment approach. Nuclear translocation of IRF3 was significantly increased in ITSCs and slice cultures treated with lipopolysaccharide (LPS) and 1,8-cineole compared with the LPS-treated cells mimicking bacterial infection. Our findings strongly suggest that 1,8-cineole potentiates the antiviral activity of IRF3 in addition to its inhibitory effect on proinflammatory NF-κB signalling, and may thus broaden its field of application | ||
| 650 | 4 | |a Journal Article | |
| 650 | 4 | |a 1 | |
| 650 | 4 | |a 8-cineole | |
| 650 | 4 | |a IRF3 | |
| 650 | 4 | |a NF-κB | |
| 650 | 4 | |a common cold | |
| 650 | 4 | |a human ex vivo model | |
| 650 | 4 | |a poly(I:C) | |
| 650 | 7 | |a Anti-Inflammatory Agents |2 NLM | |
| 650 | 7 | |a Antiviral Agents |2 NLM | |
| 650 | 7 | |a Cyclohexanols |2 NLM | |
| 650 | 7 | |a IRF3 protein, human |2 NLM | |
| 650 | 7 | |a Interferon Regulatory Factor-3 |2 NLM | |
| 650 | 7 | |a Lipopolysaccharides |2 NLM | |
| 650 | 7 | |a Monoterpenes |2 NLM | |
| 650 | 7 | |a Polynucleotides |2 NLM | |
| 650 | 7 | |a RELA protein, human |2 NLM | |
| 650 | 7 | |a Transcription Factor RelA |2 NLM | |
| 650 | 7 | |a Poly I-C |2 NLM | |
| 650 | 7 | |a O84C90HH2L |2 NLM | |
| 650 | 7 | |a Eucalyptol |2 NLM | |
| 650 | 7 | |a RV6J6604TK |2 NLM | |
| 700 | 1 | |a Greiner, Johannes F W |e verfasserin |4 aut | |
| 700 | 1 | |a Zeuner, Marie |e verfasserin |4 aut | |
| 700 | 1 | |a Brotzmann, Viktoria |e verfasserin |4 aut | |
| 700 | 1 | |a Schäfermann, Johanna |e verfasserin |4 aut | |
| 700 | 1 | |a Wieters, Frederique |e verfasserin |4 aut | |
| 700 | 1 | |a Widera, Darius |e verfasserin |4 aut | |
| 700 | 1 | |a Sudhoff, Holger |e verfasserin |4 aut | |
| 700 | 1 | |a Kaltschmidt, Barbara |e verfasserin |4 aut | |
| 700 | 1 | |a Kaltschmidt, Christian |e verfasserin |4 aut | |
| 773 | 0 | 8 | |i Enthalten in |t Clinical science (London, England : 1979) |d 1980 |g 130(2016), 15 vom: 01. Aug., Seite 1339-52 |w (DE-627)NLM000401536 |x 1470-8736 |7 nnns |
| 773 | 1 | 8 | |g volume:130 |g year:2016 |g number:15 |g day:01 |g month:08 |g pages:1339-52 |
| 856 | 4 | 0 | |u http://dx.doi.org/10.1042/CS20160218 |3 Volltext |
| 912 | |a GBV_USEFLAG_A | ||
| 912 | |a GBV_NLM | ||
| 951 | |a AR | ||
| 952 | |d 130 |j 2016 |e 15 |b 01 |c 08 |h 1339-52 | ||