LXRα represses LPS-induced inflammatory responses by competing with IRF3 for GRIP1 in Kupffer cells
Copyright © 2016 Elsevier B.V. All rights reserved..
Liver X receptors (LXRs) in the nucleus play important roles in lipid metabolism and inflammation. The mechanism of LXR regulation of the LPS-induced Toll-like receptor 4 (TLR4) inflammatory signaling pathway remains to be elucidated. C57/BL6 mice were randomly divided into four groups: control, T0901317 (a LXRs agonist), LPS and T0901317+LPS. Additionally, Kupffer cells isolated from male C57/BL6 mice were divided into the same four groups. A decreased amount of inflammatory cells infiltrated the portal areas and the hepatic sinusoids in the livers of mice in the T0901317+LPS group than in those of mice in the LPS group. In the T0901317+LPS group, the serum levels of alanine aminotransferase (ALT), aspartate aminotransferase (AST), and tumor necrosis factor alpha (TNF-α) were lower, while the serum level of interleukin-10 (IL-10) was higher. In vitro, Kupffer cells pretreated with T0901317 for 24h presented reduced TNF-α, interferon-beta (IFN-β) and interleukin-1 beta (IL-1β) levels, while the IL-10 level increased; however, the mRNA and protein expression levels of interferon regulatory factor 3 (IRF3) and glucocorticoid receptor-interacting protein 1 (GRIP1) were not significantly reduced. The co-IP data illustrated that LXRα bound to GRIP1 specifically in the T0901317+LPS group, while less IRF3 was bound to GRIP1 in the T0901317+LPS group than in the LPS group. Furthermore, the DNA-binding activity of NF-κB was decreased by pretreating Kupffer cells with T0901317 for 24h. These results suggest that activated LXRα competes with IRF3 for GRIP1 binding, thus repressing IRF3 and NF-κB transcriptional activity and inhibiting the inflammatory response initiated by LPS in Kupffer cells.
| Medienart: |
E-Artikel |
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| Erscheinungsjahr: |
2016 |
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| Erschienen: |
2016 |
| Enthalten in: |
Zur Gesamtaufnahme - volume:35 |
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| Enthalten in: |
International immunopharmacology - 35(2016) vom: 15. Juni, Seite 272-279 |
| Sprache: |
Englisch |
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| Beteiligte Personen: |
Miao, Chun-Mu [VerfasserIn] |
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| Links: |
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| Anmerkungen: |
Date Completed 21.03.2017 Date Revised 08.02.2022 published: Print-Electronic Citation Status MEDLINE |
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| doi: |
10.1016/j.intimp.2016.04.009 |
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| funding: |
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| Förderinstitution / Projekttitel: |
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| PPN (Katalog-ID): |
NLM259471585 |
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| 100 | 1 | |a Miao, Chun-Mu |e verfasserin |4 aut | |
| 245 | 1 | 0 | |a LXRα represses LPS-induced inflammatory responses by competing with IRF3 for GRIP1 in Kupffer cells |
| 264 | 1 | |c 2016 | |
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| 500 | |a Date Revised 08.02.2022 | ||
| 500 | |a published: Print-Electronic | ||
| 500 | |a Citation Status MEDLINE | ||
| 520 | |a Copyright © 2016 Elsevier B.V. All rights reserved. | ||
| 520 | |a Liver X receptors (LXRs) in the nucleus play important roles in lipid metabolism and inflammation. The mechanism of LXR regulation of the LPS-induced Toll-like receptor 4 (TLR4) inflammatory signaling pathway remains to be elucidated. C57/BL6 mice were randomly divided into four groups: control, T0901317 (a LXRs agonist), LPS and T0901317+LPS. Additionally, Kupffer cells isolated from male C57/BL6 mice were divided into the same four groups. A decreased amount of inflammatory cells infiltrated the portal areas and the hepatic sinusoids in the livers of mice in the T0901317+LPS group than in those of mice in the LPS group. In the T0901317+LPS group, the serum levels of alanine aminotransferase (ALT), aspartate aminotransferase (AST), and tumor necrosis factor alpha (TNF-α) were lower, while the serum level of interleukin-10 (IL-10) was higher. In vitro, Kupffer cells pretreated with T0901317 for 24h presented reduced TNF-α, interferon-beta (IFN-β) and interleukin-1 beta (IL-1β) levels, while the IL-10 level increased; however, the mRNA and protein expression levels of interferon regulatory factor 3 (IRF3) and glucocorticoid receptor-interacting protein 1 (GRIP1) were not significantly reduced. The co-IP data illustrated that LXRα bound to GRIP1 specifically in the T0901317+LPS group, while less IRF3 was bound to GRIP1 in the T0901317+LPS group than in the LPS group. Furthermore, the DNA-binding activity of NF-κB was decreased by pretreating Kupffer cells with T0901317 for 24h. These results suggest that activated LXRα competes with IRF3 for GRIP1 binding, thus repressing IRF3 and NF-κB transcriptional activity and inhibiting the inflammatory response initiated by LPS in Kupffer cells | ||
| 650 | 4 | |a Journal Article | |
| 650 | 4 | |a Glucocorticoid receptor-interacting protein 1 | |
| 650 | 4 | |a Interferon regulatory factor 3 | |
| 650 | 4 | |a Kupffer cell | |
| 650 | 4 | |a Liver X receptor | |
| 650 | 4 | |a Nuclear factor-kappa B | |
| 650 | 7 | |a Adaptor Proteins, Signal Transducing |2 NLM | |
| 650 | 7 | |a Cytokines |2 NLM | |
| 650 | 7 | |a Grip1 protein, mouse |2 NLM | |
| 650 | 7 | |a Hydrocarbons, Fluorinated |2 NLM | |
| 650 | 7 | |a Inflammation Mediators |2 NLM | |
| 650 | 7 | |a Interferon Regulatory Factor-3 |2 NLM | |
| 650 | 7 | |a Irf3 protein, mouse |2 NLM | |
| 650 | 7 | |a Lipopolysaccharides |2 NLM | |
| 650 | 7 | |a Liver X Receptors |2 NLM | |
| 650 | 7 | |a NF-kappa B |2 NLM | |
| 650 | 7 | |a Nerve Tissue Proteins |2 NLM | |
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| 650 | 7 | |a T0901317 |2 NLM | |
| 650 | 7 | |a Tlr4 protein, mouse |2 NLM | |
| 650 | 7 | |a Toll-Like Receptor 4 |2 NLM | |
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| 700 | 1 | |a Li, Pei-Zhi |e verfasserin |4 aut | |
| 700 | 1 | |a Liu, Zuo-Jin |e verfasserin |4 aut | |
| 700 | 1 | |a Zhu, Xi-Wen |e verfasserin |4 aut | |
| 700 | 1 | |a Ou, Zhi-Bing |e verfasserin |4 aut | |
| 700 | 1 | |a Ruan, Xiong-Zhong |e verfasserin |4 aut | |
| 700 | 1 | |a Gong, Jian-Ping |e verfasserin |4 aut | |
| 700 | 1 | |a Liu, Chang-An |e verfasserin |4 aut | |
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