Tumour-associated macrophages are recruited and differentiated in the neoplastic stroma of oral squamous cell carcinoma
Copyright © 2016 Royal College of Pathologists of Australasia. Published by Elsevier B.V. All rights reserved..
To confirm our hypothesis that macrophages recruited to fight against oral squamous cell carcinoma (SCC) invasion are functionally differentiated within neoplastic stromata, we analysed arrangements of macrophage subtypes and cancer-associated fibroblasts (CAFs) in their association with blood vasculatures in the neoplastic stroma. Surgical specimens of oral SCC were immunohistochemically examined for macrophage phenotypes (CD68, CD163, and CD204) and stromal environments (perlecan, connexin 43, and CD31). Human monocytes were co-cultured with ZK-1 cells of oral SCC origin in different culture conditions. SCC stromata were divided into two types: fascicular (fibroblast-rich) and reticular (perlecan-rich). Regardless of stromal types, CD68 positive (+)/CD163+/CD204+ macrophages were recruited when blood vessels were abundant. Connexin 43+ fibroblasts were enriched in the fascicular stroma, where blood vessels were depleted. In co-culture experiments, monocytes, in the presence of ZK-1 cells, showed TNF-α(low)/IL-12(low) and IL-10(high)/VEGF(high)/MMP-9(high) with increased expression levels for fibronectin and perlecan. With direct contact with monocytes, SCC cells also expressed CD68 and CD163. SCC stromata were characterised by CD163+/CD204+ tumour-associated macrophages (TAMs) and connexin 43+ CAFs. TAMs are differentiated from monocytes by the physical contact with oral SCC cells in the perlecan-rich neoplastic stroma, which is also induced by the cross-talk between SCC cells and stromal cells including TAMs.
| Medienart: |
E-Artikel |
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| Erscheinungsjahr: |
2016 |
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| Erschienen: |
2016 |
| Enthalten in: |
Zur Gesamtaufnahme - volume:48 |
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| Enthalten in: |
Pathology - 48(2016), 3 vom: 01. Apr., Seite 219-27 |
| Sprache: |
Englisch |
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| Beteiligte Personen: |
Essa, Ahmed Abdelaziz Mohamed [VerfasserIn] |
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| Links: |
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| Anmerkungen: |
Date Completed 17.01.2018 Date Revised 17.01.2018 published: Print-Electronic Citation Status MEDLINE |
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| doi: |
10.1016/j.pathol.2016.02.006 |
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| funding: |
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| Förderinstitution / Projekttitel: |
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| PPN (Katalog-ID): |
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| 100 | 1 | |a Essa, Ahmed Abdelaziz Mohamed |e verfasserin |4 aut | |
| 245 | 1 | 0 | |a Tumour-associated macrophages are recruited and differentiated in the neoplastic stroma of oral squamous cell carcinoma |
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| 500 | |a Date Revised 17.01.2018 | ||
| 500 | |a published: Print-Electronic | ||
| 500 | |a Citation Status MEDLINE | ||
| 520 | |a Copyright © 2016 Royal College of Pathologists of Australasia. Published by Elsevier B.V. All rights reserved. | ||
| 520 | |a To confirm our hypothesis that macrophages recruited to fight against oral squamous cell carcinoma (SCC) invasion are functionally differentiated within neoplastic stromata, we analysed arrangements of macrophage subtypes and cancer-associated fibroblasts (CAFs) in their association with blood vasculatures in the neoplastic stroma. Surgical specimens of oral SCC were immunohistochemically examined for macrophage phenotypes (CD68, CD163, and CD204) and stromal environments (perlecan, connexin 43, and CD31). Human monocytes were co-cultured with ZK-1 cells of oral SCC origin in different culture conditions. SCC stromata were divided into two types: fascicular (fibroblast-rich) and reticular (perlecan-rich). Regardless of stromal types, CD68 positive (+)/CD163+/CD204+ macrophages were recruited when blood vessels were abundant. Connexin 43+ fibroblasts were enriched in the fascicular stroma, where blood vessels were depleted. In co-culture experiments, monocytes, in the presence of ZK-1 cells, showed TNF-α(low)/IL-12(low) and IL-10(high)/VEGF(high)/MMP-9(high) with increased expression levels for fibronectin and perlecan. With direct contact with monocytes, SCC cells also expressed CD68 and CD163. SCC stromata were characterised by CD163+/CD204+ tumour-associated macrophages (TAMs) and connexin 43+ CAFs. TAMs are differentiated from monocytes by the physical contact with oral SCC cells in the perlecan-rich neoplastic stroma, which is also induced by the cross-talk between SCC cells and stromal cells including TAMs | ||
| 650 | 4 | |a Journal Article | |
| 650 | 4 | |a Research Support, Non-U.S. Gov't | |
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| 700 | 1 | |a Maruyama, Satoshi |e verfasserin |4 aut | |
| 700 | 1 | |a Abé, Tatsuya |e verfasserin |4 aut | |
| 700 | 1 | |a Babkair, Hamzah |e verfasserin |4 aut | |
| 700 | 1 | |a Raghib, Adel Mohamed |e verfasserin |4 aut | |
| 700 | 1 | |a Megahed, Eman Mohey El-Din |e verfasserin |4 aut | |
| 700 | 1 | |a Cheng, Jun |e verfasserin |4 aut | |
| 700 | 1 | |a Saku, Takashi |e verfasserin |4 aut | |
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