Experimental infection of European flat oyster Ostrea edulis with ostreid herpesvirus 1 microvar (OsHV-1μvar) : Mortality, viral load and detection of viral transcripts by in situ hybridization
Copyright © 2016 Elsevier B.V. All rights reserved..
Ostreid herpesvirus 1 (OsHV-1) infections have been reported in several bivalve species. Mortality of Pacific oyster Crassostrea gigas spat has increased considerably in Europe since 2008 linked to the spread of a variant of OsHV-1 called μvar. In the present study we demonstrated that O. edulis juveniles can be infected by OsHV-1μvar when administered as an intramuscular injection. Mortality in the oysters injected with OsHV-1μvar was first detected 4 days after injection and reached 25% mortality at day 10. Moreover, the high viral load observed and the detection of viral transcripts by in situ hybridization in several tissues of dying oysters suggested that OsHV-1μvar was the cause of mortality in the O. edulis juveniles. This is therefore the first study to provide evidence about the pathogenicity of OsHV-1μvar in a species that does not belong to the Crassostrea genus. Additionally, we present a novel method to detect OsHV-1 transcripts in infected individuals' using in situ hybridization.
| Medienart: |
E-Artikel |
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| Erscheinungsjahr: |
2016 |
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| Erschienen: |
2016 |
| Enthalten in: |
Zur Gesamtaufnahme - volume:217 |
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| Enthalten in: |
Virus research - 217(2016) vom: 02. Juni, Seite 55-62 |
| Sprache: |
Englisch |
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| Beteiligte Personen: |
López Sanmartín, Monserrat [VerfasserIn] |
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| Links: |
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| Themen: |
DNA, Viral |
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| Anmerkungen: |
Date Completed 28.11.2017 Date Revised 18.12.2017 published: Print-Electronic Citation Status MEDLINE |
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| doi: |
10.1016/j.virusres.2016.01.023 |
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| funding: |
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| Förderinstitution / Projekttitel: |
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| PPN (Katalog-ID): |
NLM258130962 |
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| 100 | 1 | |a López Sanmartín, Monserrat |e verfasserin |4 aut | |
| 245 | 1 | 0 | |a Experimental infection of European flat oyster Ostrea edulis with ostreid herpesvirus 1 microvar (OsHV-1μvar) |b Mortality, viral load and detection of viral transcripts by in situ hybridization |
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| 500 | |a Date Completed 28.11.2017 | ||
| 500 | |a Date Revised 18.12.2017 | ||
| 500 | |a published: Print-Electronic | ||
| 500 | |a Citation Status MEDLINE | ||
| 520 | |a Copyright © 2016 Elsevier B.V. All rights reserved. | ||
| 520 | |a Ostreid herpesvirus 1 (OsHV-1) infections have been reported in several bivalve species. Mortality of Pacific oyster Crassostrea gigas spat has increased considerably in Europe since 2008 linked to the spread of a variant of OsHV-1 called μvar. In the present study we demonstrated that O. edulis juveniles can be infected by OsHV-1μvar when administered as an intramuscular injection. Mortality in the oysters injected with OsHV-1μvar was first detected 4 days after injection and reached 25% mortality at day 10. Moreover, the high viral load observed and the detection of viral transcripts by in situ hybridization in several tissues of dying oysters suggested that OsHV-1μvar was the cause of mortality in the O. edulis juveniles. This is therefore the first study to provide evidence about the pathogenicity of OsHV-1μvar in a species that does not belong to the Crassostrea genus. Additionally, we present a novel method to detect OsHV-1 transcripts in infected individuals' using in situ hybridization | ||
| 650 | 4 | |a Journal Article | |
| 650 | 4 | |a Research Support, Non-U.S. Gov't | |
| 650 | 4 | |a ISH | |
| 650 | 4 | |a Microvar | |
| 650 | 4 | |a OsHV-1 | |
| 650 | 4 | |a Ostrea edulis | |
| 650 | 4 | |a Ostreid herpesvirus 1 | |
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| 700 | 1 | |a Power, Deborah M |e verfasserin |4 aut | |
| 700 | 1 | |a de la Herrán, Roberto |e verfasserin |4 aut | |
| 700 | 1 | |a Navas, José I |e verfasserin |4 aut | |
| 700 | 1 | |a Batista, Frederico M |e verfasserin |4 aut | |
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