Measurement of Antioxidant Capacity by Electron Spin Resonance Spectroscopy Based on Copper(II) Reduction
A new method is proposed for measuring the antioxidant capacity by electron spin resonance spectroscopy based on the loss of electron spin resonance signal after Cu(2+) is reduced to Cu(+) with antioxidant. Cu(+) was removed by precipitation in the presence of SCN(-). The remaining Cu(2+) was coordinated with diethyldithiocarbamate, extracted into n-butanol and determined by electron spin resonance spectrometry. Eight standards widely used in antioxidant capacity determination, including Trolox, ascorbic acid, ferulic acid, rutin, caffeic acid, quercetin, chlorogenic acid, and gallic acid were investigated. The standard curves for determining the eight standards were plotted, and results showed that the linear regression correlation coefficients were all high enough (r > 0.99). Trolox equivalent antioxidant capacity values for the antioxidant standards were calculated, and a good correlation (r > 0.94) between the values obtained by the present method and cupric reducing antioxidant capacity method was observed. The present method was applied to the analysis of real fruit samples and the evaluation of the antioxidant capacity of these fruits.
| Medienart: |
E-Artikel |
|---|
| Erscheinungsjahr: |
2016 |
|---|---|
| Erschienen: |
2016 |
| Enthalten in: |
Zur Gesamtaufnahme - volume:88 |
|---|---|
| Enthalten in: |
Analytical chemistry - 88(2016), 7 vom: 05. Apr., Seite 3885-90 |
| Sprache: |
Englisch |
|---|
| Beteiligte Personen: |
Li, Dan [VerfasserIn] |
|---|
| Links: |
|---|
| Anmerkungen: |
Date Completed 15.12.2016 Date Revised 30.12.2016 published: Print-Electronic Citation Status MEDLINE |
|---|
| doi: |
10.1021/acs.analchem.6b00049 |
|---|
| funding: |
|
|---|---|
| Förderinstitution / Projekttitel: |
|
| PPN (Katalog-ID): |
NLM257963146 |
|---|
| LEADER | 01000naa a22002652 4500 | ||
|---|---|---|---|
| 001 | NLM257963146 | ||
| 003 | DE-627 | ||
| 005 | 20231224183952.0 | ||
| 007 | cr uuu---uuuuu | ||
| 008 | 231224s2016 xx |||||o 00| ||eng c | ||
| 024 | 7 | |a 10.1021/acs.analchem.6b00049 |2 doi | |
| 028 | 5 | 2 | |a pubmed24n0859.xml |
| 035 | |a (DE-627)NLM257963146 | ||
| 035 | |a (NLM)26927869 | ||
| 040 | |a DE-627 |b ger |c DE-627 |e rakwb | ||
| 041 | |a eng | ||
| 100 | 1 | |a Li, Dan |e verfasserin |4 aut | |
| 245 | 1 | 0 | |a Measurement of Antioxidant Capacity by Electron Spin Resonance Spectroscopy Based on Copper(II) Reduction |
| 264 | 1 | |c 2016 | |
| 336 | |a Text |b txt |2 rdacontent | ||
| 337 | |a ƒaComputermedien |b c |2 rdamedia | ||
| 338 | |a ƒa Online-Ressource |b cr |2 rdacarrier | ||
| 500 | |a Date Completed 15.12.2016 | ||
| 500 | |a Date Revised 30.12.2016 | ||
| 500 | |a published: Print-Electronic | ||
| 500 | |a Citation Status MEDLINE | ||
| 520 | |a A new method is proposed for measuring the antioxidant capacity by electron spin resonance spectroscopy based on the loss of electron spin resonance signal after Cu(2+) is reduced to Cu(+) with antioxidant. Cu(+) was removed by precipitation in the presence of SCN(-). The remaining Cu(2+) was coordinated with diethyldithiocarbamate, extracted into n-butanol and determined by electron spin resonance spectrometry. Eight standards widely used in antioxidant capacity determination, including Trolox, ascorbic acid, ferulic acid, rutin, caffeic acid, quercetin, chlorogenic acid, and gallic acid were investigated. The standard curves for determining the eight standards were plotted, and results showed that the linear regression correlation coefficients were all high enough (r > 0.99). Trolox equivalent antioxidant capacity values for the antioxidant standards were calculated, and a good correlation (r > 0.94) between the values obtained by the present method and cupric reducing antioxidant capacity method was observed. The present method was applied to the analysis of real fruit samples and the evaluation of the antioxidant capacity of these fruits | ||
| 650 | 4 | |a Journal Article | |
| 650 | 4 | |a Research Support, Non-U.S. Gov't | |
| 650 | 7 | |a Antioxidants |2 NLM | |
| 650 | 7 | |a Caffeic Acids |2 NLM | |
| 650 | 7 | |a Chromans |2 NLM | |
| 650 | 7 | |a Coumaric Acids |2 NLM | |
| 650 | 7 | |a Thiocyanates |2 NLM | |
| 650 | 7 | |a Chlorogenic Acid |2 NLM | |
| 650 | 7 | |a 318ADP12RI |2 NLM | |
| 650 | 7 | |a Rutin |2 NLM | |
| 650 | 7 | |a 5G06TVY3R7 |2 NLM | |
| 650 | 7 | |a Gallic Acid |2 NLM | |
| 650 | 7 | |a 632XD903SP |2 NLM | |
| 650 | 7 | |a Copper |2 NLM | |
| 650 | 7 | |a 789U1901C5 |2 NLM | |
| 650 | 7 | |a Quercetin |2 NLM | |
| 650 | 7 | |a 9IKM0I5T1E |2 NLM | |
| 650 | 7 | |a ferulic acid |2 NLM | |
| 650 | 7 | |a AVM951ZWST |2 NLM | |
| 650 | 7 | |a Ascorbic Acid |2 NLM | |
| 650 | 7 | |a PQ6CK8PD0R |2 NLM | |
| 650 | 7 | |a 6-hydroxy-2,5,7,8-tetramethylchroman-2-carboxylic acid |2 NLM | |
| 650 | 7 | |a S18UL9710X |2 NLM | |
| 650 | 7 | |a caffeic acid |2 NLM | |
| 650 | 7 | |a U2S3A33KVM |2 NLM | |
| 700 | 1 | |a Jiang, Jia |e verfasserin |4 aut | |
| 700 | 1 | |a Han, Dandan |e verfasserin |4 aut | |
| 700 | 1 | |a Yu, Xinyu |e verfasserin |4 aut | |
| 700 | 1 | |a Wang, Kun |e verfasserin |4 aut | |
| 700 | 1 | |a Zang, Shuang |e verfasserin |4 aut | |
| 700 | 1 | |a Lu, Dayong |e verfasserin |4 aut | |
| 700 | 1 | |a Yu, Aimin |e verfasserin |4 aut | |
| 700 | 1 | |a Zhang, Ziwei |e verfasserin |4 aut | |
| 773 | 0 | 8 | |i Enthalten in |t Analytical chemistry |d 1965 |g 88(2016), 7 vom: 05. Apr., Seite 3885-90 |w (DE-627)NLM000025771 |x 1520-6882 |7 nnns |
| 773 | 1 | 8 | |g volume:88 |g year:2016 |g number:7 |g day:05 |g month:04 |g pages:3885-90 |
| 856 | 4 | 0 | |u http://dx.doi.org/10.1021/acs.analchem.6b00049 |3 Volltext |
| 912 | |a GBV_USEFLAG_A | ||
| 912 | |a GBV_NLM | ||
| 951 | |a AR | ||
| 952 | |d 88 |j 2016 |e 7 |b 05 |c 04 |h 3885-90 | ||