Inhibitory effects of S-allylmercaptocysteine against benzo(a)pyrene-induced precancerous carcinogenesis in human lung cells
Copyright © 2016 Elsevier B.V. All rights reserved..
The anti-cancer effects of oil-soluble organosulfur compounds in garlic in the initiation phase of carcinogenesis are known. However, there are few experimental studies investigating S-allylmercaptocysteine (SAMC), a water-soluble derivative of garlic. This study investigated whether SAMC prevented the carcinogen benzo(a)pyrene (B(a)P) from inducing precancerous activity in human lung cells (A549 cell line). A549 cells were either pre-treated (PreTM) or concurrently treated (CoTM) with 1μM B(a)P and either 10 or 50 μM SAMC. The 50 μM PreTM group inhibited B(a)P-induced cell proliferation by approximately 100%. The 50 μM SAMC PreTM and CoTM inhibited the B(a)P-induced G2/M phase shift by 100% and 97%, respectively. Furthermore, the PreTM and CoTM groups exhibited the potential to reduce the generation of reactive oxygen species (ROS) relative to the B(a)P group by at least 78%. The SAMC PreTM elevated superoxide dismutase (SOD) by approximately 100%. In this study, we revealed the mechanisms involved in SAMC inhibition of B(a)P-induced carcinogenesis, including suppression of cell proliferation, cell cycle regulation, attenuation of ROS formation, inhibition of DNA damage, increase of SOD activity and inhibition of nuclear factor-kappa B (NF-κB) activity. SAMC appears to be a novel therapeutic candidate for the prevention and treatment of B(a)P-induced human lung cancer.
| Medienart: |
E-Artikel |
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| Erscheinungsjahr: |
2016 |
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| Erschienen: |
2016 |
| Enthalten in: |
Zur Gesamtaufnahme - volume:34 |
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| Enthalten in: |
International immunopharmacology - 34(2016) vom: 26. Mai, Seite 37-43 |
| Sprache: |
Englisch |
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| Beteiligte Personen: |
Wang, Kaiming [VerfasserIn] |
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| Links: |
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| Anmerkungen: |
Date Completed 30.12.2016 Date Revised 11.03.2022 published: Print-Electronic Citation Status MEDLINE |
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| doi: |
10.1016/j.intimp.2016.02.017 |
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| funding: |
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| Förderinstitution / Projekttitel: |
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| PPN (Katalog-ID): |
NLM257882057 |
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| 520 | |a Copyright © 2016 Elsevier B.V. All rights reserved. | ||
| 520 | |a The anti-cancer effects of oil-soluble organosulfur compounds in garlic in the initiation phase of carcinogenesis are known. However, there are few experimental studies investigating S-allylmercaptocysteine (SAMC), a water-soluble derivative of garlic. This study investigated whether SAMC prevented the carcinogen benzo(a)pyrene (B(a)P) from inducing precancerous activity in human lung cells (A549 cell line). A549 cells were either pre-treated (PreTM) or concurrently treated (CoTM) with 1μM B(a)P and either 10 or 50 μM SAMC. The 50 μM PreTM group inhibited B(a)P-induced cell proliferation by approximately 100%. The 50 μM SAMC PreTM and CoTM inhibited the B(a)P-induced G2/M phase shift by 100% and 97%, respectively. Furthermore, the PreTM and CoTM groups exhibited the potential to reduce the generation of reactive oxygen species (ROS) relative to the B(a)P group by at least 78%. The SAMC PreTM elevated superoxide dismutase (SOD) by approximately 100%. In this study, we revealed the mechanisms involved in SAMC inhibition of B(a)P-induced carcinogenesis, including suppression of cell proliferation, cell cycle regulation, attenuation of ROS formation, inhibition of DNA damage, increase of SOD activity and inhibition of nuclear factor-kappa B (NF-κB) activity. SAMC appears to be a novel therapeutic candidate for the prevention and treatment of B(a)P-induced human lung cancer | ||
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| 700 | 1 | |a Qi, Qiuchen |e verfasserin |4 aut | |
| 700 | 1 | |a Zhang, Fang |e verfasserin |4 aut | |
| 700 | 1 | |a Zhang, Yongchun |e verfasserin |4 aut | |
| 700 | 1 | |a Zhu, Xiaosong |e verfasserin |4 aut | |
| 700 | 1 | |a Liu, Guangpu |e verfasserin |4 aut | |
| 700 | 1 | |a Luan, Yuxia |e verfasserin |4 aut | |
| 700 | 1 | |a Zhao, Zhongxi |e verfasserin |4 aut | |
| 700 | 1 | |a Cai, Jianhua |e verfasserin |4 aut | |
| 700 | 1 | |a Cao, Jimin |e verfasserin |4 aut | |
| 700 | 1 | |a Li, Shanzhong |e verfasserin |4 aut | |
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