Molecular cloning, tissue expression pattern, and copy number variation of porcine SCUBE3
The signal peptide CUB EGF-like domain-containing protein 3 (SCUBE3) gene is a member of SCUBE gene family and plays important roles in bone cell biology and the determination of limb bone length. In this study, the full-length transcript of porcine SCUBE3 was cloned using reverse transcription-polymerase chain reaction and rapid amplification of cDNA ends. The full-length sequence of porcine SCUBE3 cDNA was 4131 base pairs and included 21 exons. The SCUBE3 gene contained a 2895-base pair open reading frame that encoded a peptide of 965 amino acids. Comparison of the deduced amino acid sequences of porcine SCUBE3 with those of human, mouse, zebrafish, and rat showed 96, 95, 73, and 95% identities, respectively. Porcine SCUBE3 mRNA expression levels were highest in the backfat, bone marrow, and cartilage tissues. Copy number variation was detected in porcine SCUBE3 and validated by real-time quantitative polymerase chain reaction. Different copy number variations were present in randomly selected individuals and may, therefore, be a good marker for identifying phenotypic traits. Our findings provide a basis for further investigation of the functions and regulatory mechanisms of SCUBE3 in pigs.
| Medienart: |
E-Artikel |
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| Erscheinungsjahr: |
2016 |
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| Erschienen: |
2016 |
| Enthalten in: |
Zur Gesamtaufnahme - volume:15 |
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| Enthalten in: |
Genetics and molecular research : GMR - 15(2016), 1 vom: 05. Feb. |
| Sprache: |
Englisch |
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| Beteiligte Personen: |
Liu, X [VerfasserIn] |
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| Links: |
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| Themen: |
Journal Article |
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| Anmerkungen: |
Date Completed 13.12.2016 Date Revised 30.12.2016 published: Electronic Citation Status MEDLINE |
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| doi: |
10.4238/gmr.15017010 |
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| funding: |
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| Förderinstitution / Projekttitel: |
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| PPN (Katalog-ID): |
NLM257794530 |
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| 100 | 1 | |a Liu, X |e verfasserin |4 aut | |
| 245 | 1 | 0 | |a Molecular cloning, tissue expression pattern, and copy number variation of porcine SCUBE3 |
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| 500 | |a Date Completed 13.12.2016 | ||
| 500 | |a Date Revised 30.12.2016 | ||
| 500 | |a published: Electronic | ||
| 500 | |a Citation Status MEDLINE | ||
| 520 | |a The signal peptide CUB EGF-like domain-containing protein 3 (SCUBE3) gene is a member of SCUBE gene family and plays important roles in bone cell biology and the determination of limb bone length. In this study, the full-length transcript of porcine SCUBE3 was cloned using reverse transcription-polymerase chain reaction and rapid amplification of cDNA ends. The full-length sequence of porcine SCUBE3 cDNA was 4131 base pairs and included 21 exons. The SCUBE3 gene contained a 2895-base pair open reading frame that encoded a peptide of 965 amino acids. Comparison of the deduced amino acid sequences of porcine SCUBE3 with those of human, mouse, zebrafish, and rat showed 96, 95, 73, and 95% identities, respectively. Porcine SCUBE3 mRNA expression levels were highest in the backfat, bone marrow, and cartilage tissues. Copy number variation was detected in porcine SCUBE3 and validated by real-time quantitative polymerase chain reaction. Different copy number variations were present in randomly selected individuals and may, therefore, be a good marker for identifying phenotypic traits. Our findings provide a basis for further investigation of the functions and regulatory mechanisms of SCUBE3 in pigs | ||
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| 700 | 1 | |a Wang, L G |e verfasserin |4 aut | |
| 700 | 1 | |a Zhang, L C |e verfasserin |4 aut | |
| 700 | 1 | |a Yan, H |e verfasserin |4 aut | |
| 700 | 1 | |a Zhao, K B |e verfasserin |4 aut | |
| 700 | 1 | |a Liang, J |e verfasserin |4 aut | |
| 700 | 1 | |a Li, N |e verfasserin |4 aut | |
| 700 | 1 | |a Pu, L |e verfasserin |4 aut | |
| 700 | 1 | |a Zhang, T |e verfasserin |4 aut | |
| 700 | 1 | |a Wang, L X |e verfasserin |4 aut | |
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