Regulation of red blood cell deformability is independent of red blood cell-nitric oxide synthase under hypoxia
The aim was to study impacts of mild to severe hypoxia on human red blood cell (RBC)-nitric oxide synthase (NOS)-dependent NO production, protein S-nitrosylation and deformability.Ambient air oxygen concentration of 12 healthy subjects was step-wisely reduced from 20.95% to 16.21%, 12.35%, 10% and back to 20.95%. Additional in vitro experiments involved purging of blood (±sodium nitrite) with gas mixtures corresponding to in vivo intervention.Vital and hypoxia-associated parameters showed physiological adaptation to changing demands. Activation of RBC-NOS decreased with increasing hypoxia. RBC deformability, which is influenced by RBC-NOS activation, decreased under mild hypoxia, but surprisingly increased at severe hypoxia in vivo and in vitro. This was causatively induced by nitrite reduction to NO which increased S-nitrosylation of RBC α- and β-spectrins -a critical step to improve RBC deformability. The addition of sodium nitrite prevented decreases of RBC deformability under hypoxia by sustaining S-nitrosylation of spectrins suggesting compensatory mechanisms of non-RBC-NOS-produced NO.The results first time indicate a direct link between maintenance of RBC deformability under severe hypoxia by non-enzymatic NO production because RBC-NOS activation is reduced. These data improve our understanding of physiological mechanisms supporting adequate blood and, thus, oxygen supply to different tissues under severe hypoxia.
| Medienart: |
E-Artikel |
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| Erscheinungsjahr: |
2016 |
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| Erschienen: |
2016 |
| Enthalten in: |
Zur Gesamtaufnahme - volume:63 |
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| Enthalten in: |
Clinical hemorheology and microcirculation - 63(2016), 3 vom: 12. Sept., Seite 199-215 |
| Sprache: |
Englisch |
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| Beteiligte Personen: |
Grau, Marijke [VerfasserIn] |
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| Links: |
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| Themen: |
31C4KY9ESH |
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| Anmerkungen: |
Date Completed 16.02.2017 Date Revised 02.12.2018 published: Print Citation Status MEDLINE |
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| doi: |
10.3233/CH-162044 |
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| funding: |
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| Förderinstitution / Projekttitel: |
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| PPN (Katalog-ID): |
NLM257602941 |
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| 245 | 1 | 0 | |a Regulation of red blood cell deformability is independent of red blood cell-nitric oxide synthase under hypoxia |
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| 500 | |a Citation Status MEDLINE | ||
| 520 | |a The aim was to study impacts of mild to severe hypoxia on human red blood cell (RBC)-nitric oxide synthase (NOS)-dependent NO production, protein S-nitrosylation and deformability.Ambient air oxygen concentration of 12 healthy subjects was step-wisely reduced from 20.95% to 16.21%, 12.35%, 10% and back to 20.95%. Additional in vitro experiments involved purging of blood (±sodium nitrite) with gas mixtures corresponding to in vivo intervention.Vital and hypoxia-associated parameters showed physiological adaptation to changing demands. Activation of RBC-NOS decreased with increasing hypoxia. RBC deformability, which is influenced by RBC-NOS activation, decreased under mild hypoxia, but surprisingly increased at severe hypoxia in vivo and in vitro. This was causatively induced by nitrite reduction to NO which increased S-nitrosylation of RBC α- and β-spectrins -a critical step to improve RBC deformability. The addition of sodium nitrite prevented decreases of RBC deformability under hypoxia by sustaining S-nitrosylation of spectrins suggesting compensatory mechanisms of non-RBC-NOS-produced NO.The results first time indicate a direct link between maintenance of RBC deformability under severe hypoxia by non-enzymatic NO production because RBC-NOS activation is reduced. These data improve our understanding of physiological mechanisms supporting adequate blood and, thus, oxygen supply to different tissues under severe hypoxia | ||
| 650 | 4 | |a Journal Article | |
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| 650 | 4 | |a red blood cell deformability | |
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| 700 | 1 | |a Lauten, Alexander |e verfasserin |4 aut | |
| 700 | 1 | |a Hoeppener, Steffen |e verfasserin |4 aut | |
| 700 | 1 | |a Goebel, Bjoern |e verfasserin |4 aut | |
| 700 | 1 | |a Brenig, Julian |e verfasserin |4 aut | |
| 700 | 1 | |a Jung, Christian |e verfasserin |4 aut | |
| 700 | 1 | |a Bloch, Wilhelm |e verfasserin |4 aut | |
| 700 | 1 | |a Suhr, Frank |e verfasserin |4 aut | |
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