A General Strategy for the Discovery of Metabolic Pathways : d-Threitol, l-Threitol, and Erythritol Utilization in Mycobacterium smegmatis
We describe a general integrated bioinformatic and experimental strategy to discover the in vitro enzymatic activities and in vivo functions (metabolic pathways) of uncharacterized enzymes discovered in microbial genome projects using the ligand specificities of the solute binding proteins (SBPs) for ABC transporters. Using differential scanning fluorimetry, we determined that the SBP for an ABC transporter encoded by the genome of Mycobacterium smegmatis is stabilized by d-threitol. Using sequence similarity networks and genome neighborhood networks to guide selection of target proteins for pathway enzymes, we applied both in vitro and in vivo experimental approaches to discover novel pathways for catabolism of d-threitol, l-threitol, and erythritol.
| Errataetall: |
ErratumIn: J Am Chem Soc. 2016 Mar 30;138(12):4267. - PMID 26978037 |
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| Medienart: |
E-Artikel |
| Erscheinungsjahr: |
2015 |
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| Erschienen: |
2015 |
| Enthalten in: |
Zur Gesamtaufnahme - volume:137 |
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| Enthalten in: |
Journal of the American Chemical Society - 137(2015), 46 vom: 25. Nov., Seite 14570-3 |
| Sprache: |
Englisch |
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| Beteiligte Personen: |
Huang, Hua [VerfasserIn] |
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| Links: |
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| Themen: |
6DN82XBT5M |
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| Anmerkungen: |
Date Completed 08.07.2016 Date Revised 20.02.2019 published: Print-Electronic ErratumIn: J Am Chem Soc. 2016 Mar 30;138(12):4267. - PMID 26978037 Citation Status MEDLINE |
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| doi: |
10.1021/jacs.5b08968 |
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| funding: |
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| Förderinstitution / Projekttitel: |
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| PPN (Katalog-ID): |
NLM254556779 |
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| 500 | |a ErratumIn: J Am Chem Soc. 2016 Mar 30;138(12):4267. - PMID 26978037 | ||
| 500 | |a Citation Status MEDLINE | ||
| 520 | |a We describe a general integrated bioinformatic and experimental strategy to discover the in vitro enzymatic activities and in vivo functions (metabolic pathways) of uncharacterized enzymes discovered in microbial genome projects using the ligand specificities of the solute binding proteins (SBPs) for ABC transporters. Using differential scanning fluorimetry, we determined that the SBP for an ABC transporter encoded by the genome of Mycobacterium smegmatis is stabilized by d-threitol. Using sequence similarity networks and genome neighborhood networks to guide selection of target proteins for pathway enzymes, we applied both in vitro and in vivo experimental approaches to discover novel pathways for catabolism of d-threitol, l-threitol, and erythritol | ||
| 650 | 4 | |a Journal Article | |
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| 650 | 7 | |a threitol |2 NLM | |
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| 700 | 1 | |a Carter, Michael S |e verfasserin |4 aut | |
| 700 | 1 | |a Vetting, Matthew W |e verfasserin |4 aut | |
| 700 | 1 | |a Al-Obaidi, Nawar |e verfasserin |4 aut | |
| 700 | 1 | |a Patskovsky, Yury |e verfasserin |4 aut | |
| 700 | 1 | |a Almo, Steven C |e verfasserin |4 aut | |
| 700 | 1 | |a Gerlt, John A |e verfasserin |4 aut | |
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