CALML5 is a ZNF750- and TINCR-induced protein that binds stratifin to regulate epidermal differentiation
© 2015 Sun et al.; Published by Cold Spring Harbor Laboratory Press..
Outward migration of epidermal progenitors occurs with induction of hundreds of differentiation genes, but the identities of all regulators required for this process are unknown. We used laser capture microdissection followed by RNA sequencing to identify calmodulin-like 5 (CALML5) as the most enriched gene in differentiating outer epidermis. CALML5 mRNA was up-regulated by the ZNF750 transcription factor and then stabilized by the long noncoding RNA TINCR. CALML5 knockout impaired differentiation, abolished keratohyalin granules, and disrupted epidermal barrier function. Mass spectrometry identified SFN (stratifin/14-3-3σ) as a CALML5-binding protein. CALML5 interacts with SFN in suprabasal epidermis, cocontrols 13% of late differentiation genes, and modulates interaction of SFN to some of its binding partners. A ZNF750-TINCR-CALML5-SFN network is thus essential for epidermal differentiation.
| Medienart: |
E-Artikel |
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| Erscheinungsjahr: |
2015 |
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| Erschienen: |
2015 |
| Enthalten in: |
Zur Gesamtaufnahme - volume:29 |
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| Enthalten in: |
Genes & development - 29(2015), 21 vom: 01. Nov., Seite 2225-30 |
| Sprache: |
Englisch |
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| Beteiligte Personen: |
Sun, Bryan K [VerfasserIn] |
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| Links: |
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| Anmerkungen: |
Date Completed 23.02.2016 Date Revised 24.03.2024 published: Print SRA: SRA064531, SRA064538, SRA064547 Citation Status MEDLINE |
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| doi: |
10.1101/gad.267708.115 |
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| funding: |
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| Förderinstitution / Projekttitel: |
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| 245 | 1 | 0 | |a CALML5 is a ZNF750- and TINCR-induced protein that binds stratifin to regulate epidermal differentiation |
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| 500 | |a Citation Status MEDLINE | ||
| 520 | |a © 2015 Sun et al.; Published by Cold Spring Harbor Laboratory Press. | ||
| 520 | |a Outward migration of epidermal progenitors occurs with induction of hundreds of differentiation genes, but the identities of all regulators required for this process are unknown. We used laser capture microdissection followed by RNA sequencing to identify calmodulin-like 5 (CALML5) as the most enriched gene in differentiating outer epidermis. CALML5 mRNA was up-regulated by the ZNF750 transcription factor and then stabilized by the long noncoding RNA TINCR. CALML5 knockout impaired differentiation, abolished keratohyalin granules, and disrupted epidermal barrier function. Mass spectrometry identified SFN (stratifin/14-3-3σ) as a CALML5-binding protein. CALML5 interacts with SFN in suprabasal epidermis, cocontrols 13% of late differentiation genes, and modulates interaction of SFN to some of its binding partners. A ZNF750-TINCR-CALML5-SFN network is thus essential for epidermal differentiation | ||
| 650 | 4 | |a Journal Article | |
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| 650 | 4 | |a Research Support, U.S. Gov't, Non-P.H.S. | |
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| 700 | 1 | |a Ransohoff, Julia D |e verfasserin |4 aut | |
| 700 | 1 | |a Siprashvili, Zurab |e verfasserin |4 aut | |
| 700 | 1 | |a Qu, Kun |e verfasserin |4 aut | |
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| 700 | 1 | |a Hollmig, S Tyler |e verfasserin |4 aut | |
| 700 | 1 | |a Khavari, Paul A |e verfasserin |4 aut | |
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