Assessment of Vitamin D Metabolism by LC-MS/MS
Vitamin D is pro-hormone that has important roles in calcium metabolism in the intestine, kidneys, and bone. Many studies have indicated that vitamin D deficiency causes not only rickets and osteomalacia, but also increases the risk of various diseases such as cancer and autoimmune disease. Of the many vitamin D metabolites, 25-hydroxyvitamin D[25OH-D], 1α,25-dihydroxyvitamin D[1,25(OH)2-D], and 24,25-dihydroxyvitamin D [24,25 (OH) 2-D] are important for assessing vitamin D metabolism. They are circulating, biologically active, and major inactive forms of vitamin D metabolite, respectively. Immunoassays are widely used for the measurement of serum/plasma vitamin D metabolites. With such an assay, however, the accurate quantification of these metabolites is difficult because of cross-reactivity. Liquid chromatography-tandem mass spectrometry [LC-MS/MS] is the gold standard method for analyzing these metabolites due to its high sensitivity and selectivity. Derivatization with a Cookson-type reagent is a key technique for robust and sensitive analysis by LC-MS/MS. A Cookson-type reagent rapidly and quantitatively reacts with the s-cis-diene structure of vitamin D metabolites, and markedly enhances the ionization efficiency. Using a recently developed Cookson-type reagent (DAPTAD), we successfully established an LC-MS/MS-based method for analyzing serum vitamin D metabolites including 250H-D, 1,25 (OH) 2-D, and 24,25 (OH) 2-D. Thus, LC-MS/MS can be a powerful tool for the accurate determination of vitamin D metabolism. In this review, we describe the advantages of LC-MS/MS for the determination of vitamin D metabolites.
| Medienart: |
Artikel |
|---|
| Erscheinungsjahr: |
2015 |
|---|---|
| Erschienen: |
2015 |
| Enthalten in: |
Zur Gesamtaufnahme - volume:63 |
|---|---|
| Enthalten in: |
Rinsho byori. The Japanese journal of clinical pathology - 63(2015), 4 vom: 10. Apr., Seite 457-64 |
| Sprache: |
Japanisch |
|---|
| Beteiligte Personen: |
Ishige, Takayuki [VerfasserIn] |
|---|
| Themen: |
1406-16-2 |
|---|
| Anmerkungen: |
Date Completed 11.02.2016 Date Revised 13.08.2018 published: Print Citation Status MEDLINE |
|---|
| Förderinstitution / Projekttitel: |
|
|---|
| PPN (Katalog-ID): |
NLM254329039 |
|---|
| LEADER | 01000naa a22002652 4500 | ||
|---|---|---|---|
| 001 | NLM254329039 | ||
| 003 | DE-627 | ||
| 005 | 20231224172135.0 | ||
| 007 | tu | ||
| 008 | 231224s2015 xx ||||| 00| ||jpn c | ||
| 028 | 5 | 2 | |a pubmed24n0847.xml |
| 035 | |a (DE-627)NLM254329039 | ||
| 035 | |a (NLM)26536779 | ||
| 040 | |a DE-627 |b ger |c DE-627 |e rakwb | ||
| 041 | |a jpn | ||
| 100 | 1 | |a Ishige, Takayuki |e verfasserin |4 aut | |
| 245 | 1 | 0 | |a Assessment of Vitamin D Metabolism by LC-MS/MS |
| 264 | 1 | |c 2015 | |
| 336 | |a Text |b txt |2 rdacontent | ||
| 337 | |a ohne Hilfsmittel zu benutzen |b n |2 rdamedia | ||
| 338 | |a Band |b nc |2 rdacarrier | ||
| 500 | |a Date Completed 11.02.2016 | ||
| 500 | |a Date Revised 13.08.2018 | ||
| 500 | |a published: Print | ||
| 500 | |a Citation Status MEDLINE | ||
| 520 | |a Vitamin D is pro-hormone that has important roles in calcium metabolism in the intestine, kidneys, and bone. Many studies have indicated that vitamin D deficiency causes not only rickets and osteomalacia, but also increases the risk of various diseases such as cancer and autoimmune disease. Of the many vitamin D metabolites, 25-hydroxyvitamin D[25OH-D], 1α,25-dihydroxyvitamin D[1,25(OH)2-D], and 24,25-dihydroxyvitamin D [24,25 (OH) 2-D] are important for assessing vitamin D metabolism. They are circulating, biologically active, and major inactive forms of vitamin D metabolite, respectively. Immunoassays are widely used for the measurement of serum/plasma vitamin D metabolites. With such an assay, however, the accurate quantification of these metabolites is difficult because of cross-reactivity. Liquid chromatography-tandem mass spectrometry [LC-MS/MS] is the gold standard method for analyzing these metabolites due to its high sensitivity and selectivity. Derivatization with a Cookson-type reagent is a key technique for robust and sensitive analysis by LC-MS/MS. A Cookson-type reagent rapidly and quantitatively reacts with the s-cis-diene structure of vitamin D metabolites, and markedly enhances the ionization efficiency. Using a recently developed Cookson-type reagent (DAPTAD), we successfully established an LC-MS/MS-based method for analyzing serum vitamin D metabolites including 250H-D, 1,25 (OH) 2-D, and 24,25 (OH) 2-D. Thus, LC-MS/MS can be a powerful tool for the accurate determination of vitamin D metabolism. In this review, we describe the advantages of LC-MS/MS for the determination of vitamin D metabolites | ||
| 650 | 4 | |a Journal Article | |
| 650 | 7 | |a Reagent Kits, Diagnostic |2 NLM | |
| 650 | 7 | |a Vitamin D |2 NLM | |
| 650 | 7 | |a 1406-16-2 |2 NLM | |
| 650 | 7 | |a 24,25-Dihydroxyvitamin D 3 |2 NLM | |
| 650 | 7 | |a 40013-87-4 |2 NLM | |
| 650 | 7 | |a 25-hydroxyvitamin D |2 NLM | |
| 650 | 7 | |a A288AR3C9H |2 NLM | |
| 650 | 7 | |a Calcitriol |2 NLM | |
| 650 | 7 | |a FXC9231JVH |2 NLM | |
| 700 | 1 | |a Satoh, Mamoru |e verfasserin |4 aut | |
| 700 | 1 | |a Ogawa, Shoujiro |e verfasserin |4 aut | |
| 700 | 1 | |a Nishimura, Motoi |e verfasserin |4 aut | |
| 700 | 1 | |a Higashi, Tatsuya |e verfasserin |4 aut | |
| 700 | 1 | |a Nomura, Fumio |e verfasserin |4 aut | |
| 773 | 0 | 8 | |i Enthalten in |t Rinsho byori. The Japanese journal of clinical pathology |d 1961 |g 63(2015), 4 vom: 10. Apr., Seite 457-64 |w (DE-627)NLM000019895 |x 0047-1860 |7 nnns |
| 773 | 1 | 8 | |g volume:63 |g year:2015 |g number:4 |g day:10 |g month:04 |g pages:457-64 |
| 912 | |a GBV_USEFLAG_A | ||
| 912 | |a GBV_NLM | ||
| 951 | |a AR | ||
| 952 | |d 63 |j 2015 |e 4 |b 10 |c 04 |h 457-64 | ||