Comparative Study of Nonautolytic Mutant and Wild-Type Strains of Coprinopsis cinerea Supports an Important Role of Glucanases in Fruiting Body Autolysis
Autolysis of Coprinopsis cinerea fruiting bodies affects its commercial value. In this study, a mutant of C. cinerea that exhibits pileus expansion without pileus autolysis was obtained using ultraviolet mutagenesis. This suggests that pileus expansion and pileus autolysis involve different enzymes or proteins. Among the detected hydrolytic enzymes, only β-1,3-glucanase activity increased with expansion and autolysis of pilei in the wild-type strain, but the increase was abolished in the mutant. This suggests that β-1,3-glucanases plays a major role in the autolysis. Although there are 43 possible β-1,3-glucoside hydrolases genes, only 4 known genes, which have products that are thought to act synergistically to degrade the β-1,3-glucan backbone of cell walls during fruiting body autolysis, and an unreported gene were upregulated during pileus expansion and autolysis in the wild-type stain but were suppressed in the mutant. This suggests that expression of these β-1,3-glucanases is potentially controlled by a single regulatory mechanism.
| Medienart: |
E-Artikel |
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| Erscheinungsjahr: |
2015 |
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| Erschienen: |
2015 |
| Enthalten in: |
Zur Gesamtaufnahme - volume:63 |
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| Enthalten in: |
Journal of agricultural and food chemistry - 63(2015), 43 vom: 04. Nov., Seite 9609-14 |
| Sprache: |
Englisch |
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| Beteiligte Personen: |
Liu, Zhonghua [VerfasserIn] |
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| Links: |
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| Anmerkungen: |
Date Completed 05.08.2016 Date Revised 04.11.2015 published: Print-Electronic Citation Status MEDLINE |
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| doi: |
10.1021/acs.jafc.5b03962 |
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| funding: |
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| Förderinstitution / Projekttitel: |
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| PPN (Katalog-ID): |
NLM253519462 |
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| 245 | 1 | 0 | |a Comparative Study of Nonautolytic Mutant and Wild-Type Strains of Coprinopsis cinerea Supports an Important Role of Glucanases in Fruiting Body Autolysis |
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| 500 | |a Citation Status MEDLINE | ||
| 520 | |a Autolysis of Coprinopsis cinerea fruiting bodies affects its commercial value. In this study, a mutant of C. cinerea that exhibits pileus expansion without pileus autolysis was obtained using ultraviolet mutagenesis. This suggests that pileus expansion and pileus autolysis involve different enzymes or proteins. Among the detected hydrolytic enzymes, only β-1,3-glucanase activity increased with expansion and autolysis of pilei in the wild-type strain, but the increase was abolished in the mutant. This suggests that β-1,3-glucanases plays a major role in the autolysis. Although there are 43 possible β-1,3-glucoside hydrolases genes, only 4 known genes, which have products that are thought to act synergistically to degrade the β-1,3-glucan backbone of cell walls during fruiting body autolysis, and an unreported gene were upregulated during pileus expansion and autolysis in the wild-type stain but were suppressed in the mutant. This suggests that expression of these β-1,3-glucanases is potentially controlled by a single regulatory mechanism | ||
| 650 | 4 | |a Comparative Study | |
| 650 | 4 | |a Journal Article | |
| 650 | 4 | |a Research Support, Non-U.S. Gov't | |
| 650 | 4 | |a Coprinopsis cinerea | |
| 650 | 4 | |a fruiting body | |
| 650 | 4 | |a pileus autolysis | |
| 650 | 4 | |a pileus expansion | |
| 650 | 4 | |a β-1,3-glucoside hydrolase | |
| 650 | 7 | |a Fungal Proteins |2 NLM | |
| 650 | 7 | |a Glycoside Hydrolases |2 NLM | |
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| 700 | 1 | |a Niu, Xin |e verfasserin |4 aut | |
| 700 | 1 | |a Wang, Jun |e verfasserin |4 aut | |
| 700 | 1 | |a Zhang, Wenming |e verfasserin |4 aut | |
| 700 | 1 | |a Yang, Mingmei |e verfasserin |4 aut | |
| 700 | 1 | |a Liu, Cuicui |e verfasserin |4 aut | |
| 700 | 1 | |a Xiong, Yuanjing |e verfasserin |4 aut | |
| 700 | 1 | |a Zhao, Yan |e verfasserin |4 aut | |
| 700 | 1 | |a Pei, Siyu |e verfasserin |4 aut | |
| 700 | 1 | |a Qin, Qin |e verfasserin |4 aut | |
| 700 | 1 | |a Zhang, Yu |e verfasserin |4 aut | |
| 700 | 1 | |a Yu, Yuan |e verfasserin |4 aut | |
| 700 | 1 | |a Yuan, Sheng |e verfasserin |4 aut | |
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