Complex kinetics of DNA condensation revealed through DNA twist tracing
Toroid formation is an important mechanism for DNA condensation in cells. The length change during DNA condensation was investigated in previous single-molecule experiments. However, DNA twist is key to understanding the topological kinetics of DNA condensation. In this study, DNA twist as well as DNA length was traced during the DNA condensation by the freely orbiting magnetic tweezers and the tilted magnetic tweezers combined with Brownian dynamics simulations. The experimental results disclose the complex relationship between DNA extension and backbone rotation. Brownian dynamics simulations show that the toroid formation follows a wiggling pathway which leads to the complex DNA backbone rotation as revealed in our experiments. These findings provide the complete description of multivalent cation-dependent DNA toroid formation under tension.
| Medienart: |
E-Artikel |
|---|
| Erscheinungsjahr: |
2015 |
|---|---|
| Erschienen: |
2015 |
| Enthalten in: |
Zur Gesamtaufnahme - volume:92 |
|---|---|
| Enthalten in: |
Physical review. E, Statistical, nonlinear, and soft matter physics - 92(2015), 2 vom: 26. Aug., Seite 022707 |
| Sprache: |
Englisch |
|---|
| Beteiligte Personen: |
Li, Wei [VerfasserIn] |
|---|
| Themen: |
9007-49-2 |
|---|
| Anmerkungen: |
Date Completed 21.06.2016 Date Revised 19.09.2015 published: Print-Electronic Citation Status MEDLINE |
|---|
| Förderinstitution / Projekttitel: |
|
|---|
| PPN (Katalog-ID): |
NLM252873564 |
|---|
| LEADER | 01000naa a22002652 4500 | ||
|---|---|---|---|
| 001 | NLM252873564 | ||
| 003 | DE-627 | ||
| 005 | 20231224165027.0 | ||
| 007 | cr uuu---uuuuu | ||
| 008 | 231224s2015 xx |||||o 00| ||eng c | ||
| 028 | 5 | 2 | |a pubmed24n0842.xml |
| 035 | |a (DE-627)NLM252873564 | ||
| 035 | |a (NLM)26382432 | ||
| 040 | |a DE-627 |b ger |c DE-627 |e rakwb | ||
| 041 | |a eng | ||
| 100 | 1 | |a Li, Wei |e verfasserin |4 aut | |
| 245 | 1 | 0 | |a Complex kinetics of DNA condensation revealed through DNA twist tracing |
| 264 | 1 | |c 2015 | |
| 336 | |a Text |b txt |2 rdacontent | ||
| 337 | |a ƒaComputermedien |b c |2 rdamedia | ||
| 338 | |a ƒa Online-Ressource |b cr |2 rdacarrier | ||
| 500 | |a Date Completed 21.06.2016 | ||
| 500 | |a Date Revised 19.09.2015 | ||
| 500 | |a published: Print-Electronic | ||
| 500 | |a Citation Status MEDLINE | ||
| 520 | |a Toroid formation is an important mechanism for DNA condensation in cells. The length change during DNA condensation was investigated in previous single-molecule experiments. However, DNA twist is key to understanding the topological kinetics of DNA condensation. In this study, DNA twist as well as DNA length was traced during the DNA condensation by the freely orbiting magnetic tweezers and the tilted magnetic tweezers combined with Brownian dynamics simulations. The experimental results disclose the complex relationship between DNA extension and backbone rotation. Brownian dynamics simulations show that the toroid formation follows a wiggling pathway which leads to the complex DNA backbone rotation as revealed in our experiments. These findings provide the complete description of multivalent cation-dependent DNA toroid formation under tension | ||
| 650 | 4 | |a Journal Article | |
| 650 | 4 | |a Research Support, Non-U.S. Gov't | |
| 650 | 7 | |a DNA |2 NLM | |
| 650 | 7 | |a 9007-49-2 |2 NLM | |
| 650 | 7 | |a Streptavidin |2 NLM | |
| 650 | 7 | |a 9013-20-1 |2 NLM | |
| 650 | 7 | |a Digoxigenin |2 NLM | |
| 650 | 7 | |a NQ1SX9LNAU |2 NLM | |
| 700 | 1 | |a Wong, Wei Juan |e verfasserin |4 aut | |
| 700 | 1 | |a Lim, Ci Ji |e verfasserin |4 aut | |
| 700 | 1 | |a Ju, Hai-Peng |e verfasserin |4 aut | |
| 700 | 1 | |a Li, Ming |e verfasserin |4 aut | |
| 700 | 1 | |a Yan, Jie |e verfasserin |4 aut | |
| 700 | 1 | |a Wang, Peng-Ye |e verfasserin |4 aut | |
| 773 | 0 | 8 | |i Enthalten in |t Physical review. E, Statistical, nonlinear, and soft matter physics |d 2001 |g 92(2015), 2 vom: 26. Aug., Seite 022707 |w (DE-627)NLM112130313 |x 1550-2376 |7 nnns |
| 773 | 1 | 8 | |g volume:92 |g year:2015 |g number:2 |g day:26 |g month:08 |g pages:022707 |
| 912 | |a GBV_USEFLAG_A | ||
| 912 | |a GBV_NLM | ||
| 951 | |a AR | ||
| 952 | |d 92 |j 2015 |e 2 |b 26 |c 08 |h 022707 | ||