Successful cross-protective efficacy induced by heat-adapted live attenuated nephropathogenic infectious bronchitis virus derived from a natural recombinant strain
Copyright © 2015 Elsevier Ltd. All rights reserved..
A natural recombinant nephropathogenic K40/09 strain of infectious bronchitis virus (IBV) was heat-adapted for possible future use as live attenuated vaccine. The K40/09 strain was selected during successive serial passages in specific-pathogen free (SPF) embryonated eggs at sub-optimal higher temperature (56°C). Unlike the parental strain, the attenuated strain, designated K40/09 HP50, was found to be safe in 1-day-old SPF chicks, which showed neither mortality nor signs of morbidity, and rarely induced ciliostasis or histological changes in the trachea and kidney after intraocular and fine-spray administration. K40/09 HP50 provided almost complete protection against two distinct subgroups of a nephropathogenic strain (KM91-like and QX-like subgroup) and elicited the production of high titers of neutralizing antibody (neutralization index of 3.6). We conclude that the K40/09 HP50 vaccine virus is rapidly attenuated by heat adaptation and exhibits the desired level of attenuation, immunogenicity, and protective efficacy required for a live attenuated vaccine. These results indicate that the K40/09 vaccine could be helpful for the reduction of economic losses caused by recently emergent nephropathogenic IBV infection in many countries.
| Medienart: |
E-Artikel |
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| Erscheinungsjahr: |
2015 |
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| Erschienen: |
2015 |
| Enthalten in: |
Zur Gesamtaufnahme - volume:33 |
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| Enthalten in: |
Vaccine - 33(2015), 51 vom: 16. Dez., Seite 7370-7374 |
| Sprache: |
Englisch |
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| Beteiligte Personen: |
Lim, Tae-Hyun [VerfasserIn] |
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| Links: |
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| Themen: |
Attenuation |
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| Anmerkungen: |
Date Completed 05.10.2016 Date Revised 09.04.2020 published: Print-Electronic Citation Status MEDLINE |
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| doi: |
10.1016/j.vaccine.2015.07.043 |
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| funding: |
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| Förderinstitution / Projekttitel: |
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| PPN (Katalog-ID): |
NLM251309215 |
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| 245 | 1 | 0 | |a Successful cross-protective efficacy induced by heat-adapted live attenuated nephropathogenic infectious bronchitis virus derived from a natural recombinant strain |
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| 520 | |a Copyright © 2015 Elsevier Ltd. All rights reserved. | ||
| 520 | |a A natural recombinant nephropathogenic K40/09 strain of infectious bronchitis virus (IBV) was heat-adapted for possible future use as live attenuated vaccine. The K40/09 strain was selected during successive serial passages in specific-pathogen free (SPF) embryonated eggs at sub-optimal higher temperature (56°C). Unlike the parental strain, the attenuated strain, designated K40/09 HP50, was found to be safe in 1-day-old SPF chicks, which showed neither mortality nor signs of morbidity, and rarely induced ciliostasis or histological changes in the trachea and kidney after intraocular and fine-spray administration. K40/09 HP50 provided almost complete protection against two distinct subgroups of a nephropathogenic strain (KM91-like and QX-like subgroup) and elicited the production of high titers of neutralizing antibody (neutralization index of 3.6). We conclude that the K40/09 HP50 vaccine virus is rapidly attenuated by heat adaptation and exhibits the desired level of attenuation, immunogenicity, and protective efficacy required for a live attenuated vaccine. These results indicate that the K40/09 vaccine could be helpful for the reduction of economic losses caused by recently emergent nephropathogenic IBV infection in many countries | ||
| 650 | 4 | |a Journal Article | |
| 650 | 4 | |a Attenuation | |
| 650 | 4 | |a Cross-protective efficacy | |
| 650 | 4 | |a Heat adaptation | |
| 650 | 4 | |a Infectious bronchitis virus | |
| 650 | 4 | |a Safety | |
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| 650 | 7 | |a Viral Vaccines |2 NLM | |
| 700 | 1 | |a Youn, Ha-Na |e verfasserin |4 aut | |
| 700 | 1 | |a Yuk, Seong-Su |e verfasserin |4 aut | |
| 700 | 1 | |a Kwon, Jung-Hoon |e verfasserin |4 aut | |
| 700 | 1 | |a Hong, Woo-Tack |e verfasserin |4 aut | |
| 700 | 1 | |a Gwon, Gyeong-Bin |e verfasserin |4 aut | |
| 700 | 1 | |a Lee, Jung-Ah |e verfasserin |4 aut | |
| 700 | 1 | |a Lee, Joong-Bok |e verfasserin |4 aut | |
| 700 | 1 | |a Lee, Sang-Won |e verfasserin |4 aut | |
| 700 | 1 | |a Song, Chang-Seon |e verfasserin |4 aut | |
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