Elastic hydrogel substrate supports robust expansion of murine myoblasts and enhances their engraftment
Copyright © 2015 Elsevier Inc. All rights reserved..
The application of satellite cell-derived myoblasts in regenerative medicine has been restricted by the rapid loss of stemness during in vitro cell expansion using traditional culture systems. However, studies published in the past decade have highlighted the influence of substrate elasticity on stem cell fate and revealed that culture on a soft hydrogel substrate can promote self-renewal and prolong the regenerative potential of muscle stem cells. Whether hydrogel substrates have similar effects after long-term robust expansion remains to be determined. Herein we prepared an elastic chitosan/beta-glycerophosphate/collagen hydrogel mimicking the soft microenvironment of muscle tissues for use as the substrate for satellite cell culture and investigated its influence on long-term cell expansion. After 20 passages in culture, satellite cell-derived myoblasts cultured on our hydrogel substrate exhibited significant improvements in proliferation capability, cell viability, colony forming frequency, and potential for myogenic differentiation compared to those cultured on a routine rigid culture surface. Immunochemical staining and western blot analysis both confirmed that myoblasts cultured on the hydrogel substrate expressed higher levels of several differentiation-related markers, including Pax7, Pax3, and SSEA-1, and a lower level of MyoD compared to myoblasts cultured on rigid culture plates (all p<0.05). After transplantation into the tibialis anterior of nude mice, myoblasts that had been cultured on the hydrogel substrate demonstrated a significantly greater engraftment efficacy than those cultured on the traditional surface. Collectively, these results indicate that the elastic hydrogel substrate supported robust expansion of murine myoblasts and enhanced their engraftment in vivo.
| Medienart: |
E-Artikel |
|---|
| Erscheinungsjahr: |
2015 |
|---|---|
| Erschienen: |
2015 |
| Enthalten in: |
Zur Gesamtaufnahme - volume:337 |
|---|---|
| Enthalten in: |
Experimental cell research - 337(2015), 1 vom: 10. Sept., Seite 111-9 |
| Sprache: |
Englisch |
|---|
| Beteiligte Personen: |
Ding, Ke [VerfasserIn] |
|---|
| Links: |
|---|
| Themen: |
Antigens, Differentiation |
|---|
| Anmerkungen: |
Date Completed 20.11.2015 Date Revised 03.09.2015 published: Print-Electronic Citation Status MEDLINE |
|---|
| doi: |
10.1016/j.yexcr.2015.07.021 |
|---|
| funding: |
|
|---|---|
| Förderinstitution / Projekttitel: |
|
| PPN (Katalog-ID): |
NLM251229947 |
|---|
| LEADER | 01000naa a22002652 4500 | ||
|---|---|---|---|
| 001 | NLM251229947 | ||
| 003 | DE-627 | ||
| 005 | 20231224161541.0 | ||
| 007 | cr uuu---uuuuu | ||
| 008 | 231224s2015 xx |||||o 00| ||eng c | ||
| 024 | 7 | |a 10.1016/j.yexcr.2015.07.021 |2 doi | |
| 028 | 5 | 2 | |a pubmed24n0837.xml |
| 035 | |a (DE-627)NLM251229947 | ||
| 035 | |a (NLM)26210646 | ||
| 035 | |a (PII)S0014-4827(15)30051-3 | ||
| 040 | |a DE-627 |b ger |c DE-627 |e rakwb | ||
| 041 | |a eng | ||
| 100 | 1 | |a Ding, Ke |e verfasserin |4 aut | |
| 245 | 1 | 0 | |a Elastic hydrogel substrate supports robust expansion of murine myoblasts and enhances their engraftment |
| 264 | 1 | |c 2015 | |
| 336 | |a Text |b txt |2 rdacontent | ||
| 337 | |a ƒaComputermedien |b c |2 rdamedia | ||
| 338 | |a ƒa Online-Ressource |b cr |2 rdacarrier | ||
| 500 | |a Date Completed 20.11.2015 | ||
| 500 | |a Date Revised 03.09.2015 | ||
| 500 | |a published: Print-Electronic | ||
| 500 | |a Citation Status MEDLINE | ||
| 520 | |a Copyright © 2015 Elsevier Inc. All rights reserved. | ||
| 520 | |a The application of satellite cell-derived myoblasts in regenerative medicine has been restricted by the rapid loss of stemness during in vitro cell expansion using traditional culture systems. However, studies published in the past decade have highlighted the influence of substrate elasticity on stem cell fate and revealed that culture on a soft hydrogel substrate can promote self-renewal and prolong the regenerative potential of muscle stem cells. Whether hydrogel substrates have similar effects after long-term robust expansion remains to be determined. Herein we prepared an elastic chitosan/beta-glycerophosphate/collagen hydrogel mimicking the soft microenvironment of muscle tissues for use as the substrate for satellite cell culture and investigated its influence on long-term cell expansion. After 20 passages in culture, satellite cell-derived myoblasts cultured on our hydrogel substrate exhibited significant improvements in proliferation capability, cell viability, colony forming frequency, and potential for myogenic differentiation compared to those cultured on a routine rigid culture surface. Immunochemical staining and western blot analysis both confirmed that myoblasts cultured on the hydrogel substrate expressed higher levels of several differentiation-related markers, including Pax7, Pax3, and SSEA-1, and a lower level of MyoD compared to myoblasts cultured on rigid culture plates (all p<0.05). After transplantation into the tibialis anterior of nude mice, myoblasts that had been cultured on the hydrogel substrate demonstrated a significantly greater engraftment efficacy than those cultured on the traditional surface. Collectively, these results indicate that the elastic hydrogel substrate supported robust expansion of murine myoblasts and enhanced their engraftment in vivo | ||
| 650 | 4 | |a Journal Article | |
| 650 | 4 | |a Research Support, Non-U.S. Gov't | |
| 650 | 4 | |a Cell culture | |
| 650 | 4 | |a Cell therapy | |
| 650 | 4 | |a Cell transplantation | |
| 650 | 4 | |a Elasticity | |
| 650 | 4 | |a Myoblast | |
| 650 | 4 | |a Stemness | |
| 650 | 7 | |a Antigens, Differentiation |2 NLM | |
| 650 | 7 | |a Hydrogels |2 NLM | |
| 700 | 1 | |a Yang, Zhong |e verfasserin |4 aut | |
| 700 | 1 | |a Xu, Jian-Zhong |e verfasserin |4 aut | |
| 700 | 1 | |a Liu, Wen-Ying |e verfasserin |4 aut | |
| 700 | 1 | |a Zeng, Qiang |e verfasserin |4 aut | |
| 700 | 1 | |a Hou, Fang |e verfasserin |4 aut | |
| 700 | 1 | |a Lin, Sen |e verfasserin |4 aut | |
| 773 | 0 | 8 | |i Enthalten in |t Experimental cell research |d 1953 |g 337(2015), 1 vom: 10. Sept., Seite 111-9 |w (DE-627)NLM000003123 |x 1090-2422 |7 nnns |
| 773 | 1 | 8 | |g volume:337 |g year:2015 |g number:1 |g day:10 |g month:09 |g pages:111-9 |
| 856 | 4 | 0 | |u http://dx.doi.org/10.1016/j.yexcr.2015.07.021 |3 Volltext |
| 912 | |a GBV_USEFLAG_A | ||
| 912 | |a GBV_NLM | ||
| 951 | |a AR | ||
| 952 | |d 337 |j 2015 |e 1 |b 10 |c 09 |h 111-9 | ||