Does the liposuction method influence the phenotypic characteristic of human adipose-derived stem cells?
© 2015 The Authors..
Adipose-derived stem cells (ASCs) possess a high differentiation and proliferation potential. However, the phenotypic characterization of ASCs is still difficult. Until now, there is no extensive analysis of ASCs markers depending on different liposuction methods. Therefore, the aim of the present study was to analyse 242 surface markers and determine the differences in the phenotypic pattern between ASCs obtained during mechanical and ultrasound-assisted liposuction. ASCs were isolated from healthy donors, due to mechanical and ultrasound-assisted liposuction and cultured in standard medium to the second passage. Differentiation potential and markers expression was evaluated to confirm the mesenchymal nature of cells. Then, the BD LyoplateTM Human Cell Surface Marker Screening Panel was used. Results shown that both population of ASCs are characterized by high expression of markers specific for ASCs: cluster of differentiation (CD)9, CD10, CD34, CD44, CD49d, CD54, CD55, CD59, CD71 and low expression of CD11a, CD11c and CD144. Moreover, we have noticed significant differences in antigen expression in 58 markers from the 242 studied. Presented study shows for the first time that different liposuction methods are not a significant factor which can influence the expression of human ASCs surface markers.
| Medienart: |
E-Artikel |
|---|
| Erscheinungsjahr: |
2015 |
|---|---|
| Erschienen: |
2015 |
| Enthalten in: |
Zur Gesamtaufnahme - volume:35 |
|---|---|
| Enthalten in: |
Bioscience reports - 35(2015), 3 vom: 14. Mai |
| Sprache: |
Englisch |
|---|
| Beteiligte Personen: |
Bajek, Anna [VerfasserIn] |
|---|
| Links: |
|---|
| Themen: |
Adipose-derived stem cells |
|---|
| Anmerkungen: |
Date Completed 13.12.2016 Date Revised 13.11.2018 published: Electronic Citation Status MEDLINE |
|---|
| doi: |
10.1042/BSR20150067 |
|---|
| funding: |
|
|---|---|
| Förderinstitution / Projekttitel: |
|
| PPN (Katalog-ID): |
NLM250955539 |
|---|
| LEADER | 01000naa a22002652 4500 | ||
|---|---|---|---|
| 001 | NLM250955539 | ||
| 003 | DE-627 | ||
| 005 | 20231224160934.0 | ||
| 007 | cr uuu---uuuuu | ||
| 008 | 231224s2015 xx |||||o 00| ||eng c | ||
| 024 | 7 | |a 10.1042/BSR20150067 |2 doi | |
| 028 | 5 | 2 | |a pubmed24n0836.xml |
| 035 | |a (DE-627)NLM250955539 | ||
| 035 | |a (NLM)26182374 | ||
| 035 | |a (PII)e00212 | ||
| 040 | |a DE-627 |b ger |c DE-627 |e rakwb | ||
| 041 | |a eng | ||
| 100 | 1 | |a Bajek, Anna |e verfasserin |4 aut | |
| 245 | 1 | 0 | |a Does the liposuction method influence the phenotypic characteristic of human adipose-derived stem cells? |
| 264 | 1 | |c 2015 | |
| 336 | |a Text |b txt |2 rdacontent | ||
| 337 | |a ƒaComputermedien |b c |2 rdamedia | ||
| 338 | |a ƒa Online-Ressource |b cr |2 rdacarrier | ||
| 500 | |a Date Completed 13.12.2016 | ||
| 500 | |a Date Revised 13.11.2018 | ||
| 500 | |a published: Electronic | ||
| 500 | |a Citation Status MEDLINE | ||
| 520 | |a © 2015 The Authors. | ||
| 520 | |a Adipose-derived stem cells (ASCs) possess a high differentiation and proliferation potential. However, the phenotypic characterization of ASCs is still difficult. Until now, there is no extensive analysis of ASCs markers depending on different liposuction methods. Therefore, the aim of the present study was to analyse 242 surface markers and determine the differences in the phenotypic pattern between ASCs obtained during mechanical and ultrasound-assisted liposuction. ASCs were isolated from healthy donors, due to mechanical and ultrasound-assisted liposuction and cultured in standard medium to the second passage. Differentiation potential and markers expression was evaluated to confirm the mesenchymal nature of cells. Then, the BD LyoplateTM Human Cell Surface Marker Screening Panel was used. Results shown that both population of ASCs are characterized by high expression of markers specific for ASCs: cluster of differentiation (CD)9, CD10, CD34, CD44, CD49d, CD54, CD55, CD59, CD71 and low expression of CD11a, CD11c and CD144. Moreover, we have noticed significant differences in antigen expression in 58 markers from the 242 studied. Presented study shows for the first time that different liposuction methods are not a significant factor which can influence the expression of human ASCs surface markers | ||
| 650 | 4 | |a Journal Article | |
| 650 | 4 | |a Research Support, Non-U.S. Gov't | |
| 650 | 4 | |a adipose-derived stem cells | |
| 650 | 4 | |a liposuction | |
| 650 | 4 | |a markers' extensive analysis | |
| 650 | 4 | |a phenotypic characterization | |
| 650 | 7 | |a Antigens, CD |2 NLM | |
| 650 | 7 | |a Biomarkers |2 NLM | |
| 700 | 1 | |a Gurtowska, Natalia |e verfasserin |4 aut | |
| 700 | 1 | |a Gackowska, Lidia |e verfasserin |4 aut | |
| 700 | 1 | |a Kubiszewska, Izabela |e verfasserin |4 aut | |
| 700 | 1 | |a Bodnar, Magdalena |e verfasserin |4 aut | |
| 700 | 1 | |a Marszałek, Andrzej |e verfasserin |4 aut | |
| 700 | 1 | |a Januszewski, Rafał |e verfasserin |4 aut | |
| 700 | 1 | |a Michalkiewicz, Jacek |e verfasserin |4 aut | |
| 700 | 1 | |a Drewa, Tomasz |e verfasserin |4 aut | |
| 773 | 0 | 8 | |i Enthalten in |t Bioscience reports |d 1981 |g 35(2015), 3 vom: 14. Mai |w (DE-627)NLM01264465X |x 1573-4935 |7 nnns |
| 773 | 1 | 8 | |g volume:35 |g year:2015 |g number:3 |g day:14 |g month:05 |
| 856 | 4 | 0 | |u http://dx.doi.org/10.1042/BSR20150067 |3 Volltext |
| 912 | |a GBV_USEFLAG_A | ||
| 912 | |a GBV_NLM | ||
| 951 | |a AR | ||
| 952 | |d 35 |j 2015 |e 3 |b 14 |c 05 | ||