High prevalence of primary drug resistance in children with intrathoracic tuberculosis in India
BACKGROUND: Drug susceptibility testing (DST) of Mycobacterium tuberculosis (Mtb) isolates is crucial for the effective treatment of tuberculosis. Data on DST patterns in Mtb isolates in childhood tuberculosis are scanty.
AIMS: To determine drug resistance patterns in Mtb isolates from a paediatric TB cohort in North India.
METHODS: 403 children aged 6 months to14 year with probable intrathoracic tuberculosis were enrolled prospectively. All were treatment-naïve. 802 ambulatory-induced sputa (IS) and 787 gastric aspirate (GA) samples were cultured in BACTEC-MGIT960 system, and DST of the Mtb isolates was undertaken using the automated BACTEC-MGIT960 SIRE kit.
RESULTS: Of the 403 children, 147 (36.4%) were culture-confirmed: 132 (89.8%) isolates were Mtb and 15 (10.2%) non-tuberculous mycobacteria (NTM). Five Mtb isolates were contaminated and the remaining 127 were subjected to in-vitro drug susceptibility testing against streptomycin, isoniazid, rifampicin and ethambutol. Twenty-six (20.47%) isolates were resistant to one or more drugs, seven (5.5%) were resistant to rifampicin singly or in combination, and 11 (8.7%) were resistant to isoniazid singly or in combination. Mono-resistance to isoniazid, rifampicin, streptomycin and ethambutol was detected in four (3.1%), one (0.8%), four (3.1%) and two (1.6%), respectively. Five children (3.9%) had MDR-TB; 101 (79.9%) children had Mtb isolates which were sensitive to all four drugs.
CONCLUSIONS: The rifampicin and isoniazid resistance rates were much higher than those in the adult TB population in India.
| Medienart: |
E-Artikel |
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| Erscheinungsjahr: |
2016 |
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| Erschienen: |
2016 |
| Enthalten in: |
Zur Gesamtaufnahme - volume:36 |
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| Enthalten in: |
Paediatrics and international child health - 36(2016), 3 vom: 02. Aug., Seite 214-8 |
| Sprache: |
Englisch |
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| Beteiligte Personen: |
Prajapati, Suneel [VerfasserIn] |
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| Links: |
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| Themen: |
Antitubercular Agents |
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| Anmerkungen: |
Date Completed 31.01.2017 Date Revised 31.01.2017 published: Print Citation Status MEDLINE |
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| doi: |
10.1179/2046905515Y.0000000041 |
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| funding: |
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| Förderinstitution / Projekttitel: |
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| PPN (Katalog-ID): |
NLM249748339 |
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| 500 | |a Date Revised 31.01.2017 | ||
| 500 | |a published: Print | ||
| 500 | |a Citation Status MEDLINE | ||
| 520 | |a BACKGROUND: Drug susceptibility testing (DST) of Mycobacterium tuberculosis (Mtb) isolates is crucial for the effective treatment of tuberculosis. Data on DST patterns in Mtb isolates in childhood tuberculosis are scanty | ||
| 520 | |a AIMS: To determine drug resistance patterns in Mtb isolates from a paediatric TB cohort in North India | ||
| 520 | |a METHODS: 403 children aged 6 months to14 year with probable intrathoracic tuberculosis were enrolled prospectively. All were treatment-naïve. 802 ambulatory-induced sputa (IS) and 787 gastric aspirate (GA) samples were cultured in BACTEC-MGIT960 system, and DST of the Mtb isolates was undertaken using the automated BACTEC-MGIT960 SIRE kit | ||
| 520 | |a RESULTS: Of the 403 children, 147 (36.4%) were culture-confirmed: 132 (89.8%) isolates were Mtb and 15 (10.2%) non-tuberculous mycobacteria (NTM). Five Mtb isolates were contaminated and the remaining 127 were subjected to in-vitro drug susceptibility testing against streptomycin, isoniazid, rifampicin and ethambutol. Twenty-six (20.47%) isolates were resistant to one or more drugs, seven (5.5%) were resistant to rifampicin singly or in combination, and 11 (8.7%) were resistant to isoniazid singly or in combination. Mono-resistance to isoniazid, rifampicin, streptomycin and ethambutol was detected in four (3.1%), one (0.8%), four (3.1%) and two (1.6%), respectively. Five children (3.9%) had MDR-TB; 101 (79.9%) children had Mtb isolates which were sensitive to all four drugs | ||
| 520 | |a CONCLUSIONS: The rifampicin and isoniazid resistance rates were much higher than those in the adult TB population in India | ||
| 650 | 4 | |a Journal Article | |
| 650 | 4 | |a Gastric lavage | |
| 650 | 4 | |a India | |
| 650 | 4 | |a Induced sputum | |
| 650 | 4 | |a Multi-drug-resistant TB | |
| 650 | 4 | |a Rifampicin | |
| 650 | 4 | |a Tuberculosis | |
| 650 | 7 | |a Antitubercular Agents |2 NLM | |
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| 700 | 1 | |a Mukherjee, Aparna |e verfasserin |4 aut | |
| 700 | 1 | |a Kabra, S K |e verfasserin |4 aut | |
| 700 | 1 | |a Lodha, Rakesh |e verfasserin |4 aut | |
| 700 | 1 | |a Singh, Varinder |e verfasserin |4 aut | |
| 700 | 1 | |a Grewal, Harleen M S |e verfasserin |4 aut | |
| 700 | 1 | |a Singh, Sarman |e verfasserin |4 aut | |
| 700 | 0 | |a Delhi Pediatric TB Study Group |e verfasserin |4 aut | |
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