PURE-LAMP Procedure for the Diagnosis of Extrapulmonary Tuberculosis : A Case Series
Although the polymerase chain reaction is effective for the diagnosis of extrapulmonary tuberculosis (EPTB), it is typically unavailable in resource-limited situations. In contrast, the loop-mediated isothermal amplification (LAMP) assay is a relatively cost-effective and accessible method. Additionally, when combined with the procedure for ultra-rapid extraction (PURE) kit, which enables simple DNA extraction, LAMP can detect Mycobacterium tuberculosis in sputum within 1.5 hours using a simple procedure. In this study, we investigated the utility of the PURE-LAMP technique to diagnose three cases of EPTB and showed that it may potentially be a valuable tool for the diagnosis of EPTB.
| Medienart: |
E-Artikel |
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| Erscheinungsjahr: |
2015 |
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| Erschienen: |
2015 |
| Enthalten in: |
Zur Gesamtaufnahme - volume:54 |
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| Enthalten in: |
Internal medicine (Tokyo, Japan) - 54(2015), 11 vom: 01., Seite 1447-50 |
| Sprache: |
Englisch |
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| Beteiligte Personen: |
Kouzaki, Yuji [VerfasserIn] |
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| Links: |
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| Themen: |
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| Anmerkungen: |
Date Completed 13.01.2016 Date Revised 02.12.2018 published: Print-Electronic Citation Status MEDLINE |
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| doi: |
10.2169/internalmedicine.54.4615 |
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| funding: |
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| Förderinstitution / Projekttitel: |
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| PPN (Katalog-ID): |
NLM249508206 |
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| 500 | |a published: Print-Electronic | ||
| 500 | |a Citation Status MEDLINE | ||
| 520 | |a Although the polymerase chain reaction is effective for the diagnosis of extrapulmonary tuberculosis (EPTB), it is typically unavailable in resource-limited situations. In contrast, the loop-mediated isothermal amplification (LAMP) assay is a relatively cost-effective and accessible method. Additionally, when combined with the procedure for ultra-rapid extraction (PURE) kit, which enables simple DNA extraction, LAMP can detect Mycobacterium tuberculosis in sputum within 1.5 hours using a simple procedure. In this study, we investigated the utility of the PURE-LAMP technique to diagnose three cases of EPTB and showed that it may potentially be a valuable tool for the diagnosis of EPTB | ||
| 650 | 4 | |a Case Reports | |
| 650 | 4 | |a Journal Article | |
| 700 | 1 | |a Mikita, Kei |e verfasserin |4 aut | |
| 700 | 1 | |a Maeda, Takuya |e verfasserin |4 aut | |
| 700 | 1 | |a Ishihara, Masashi |e verfasserin |4 aut | |
| 700 | 1 | |a Kawano, Shuichi |e verfasserin |4 aut | |
| 700 | 1 | |a Fujikura, Yuji |e verfasserin |4 aut | |
| 700 | 1 | |a Imai, Kazuo |e verfasserin |4 aut | |
| 700 | 1 | |a Kanoh, Soichiro |e verfasserin |4 aut | |
| 700 | 1 | |a Utsunomiya, Kazuho |e verfasserin |4 aut | |
| 700 | 1 | |a Inoue, Masahiro |e verfasserin |4 aut | |
| 700 | 1 | |a Miyahira, Yasushi |e verfasserin |4 aut | |
| 700 | 1 | |a Kawana, Akihiko |e verfasserin |4 aut | |
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