A Single Point Mutation Creating a Furin Cleavage Site in the Spike Protein Renders Porcine Epidemic Diarrhea Coronavirus Trypsin Independent for Cell Entry and Fusion
Copyright © 2015, American Society for Microbiology. All Rights Reserved..
The emerging porcine epidemic diarrhea virus (PEDV) requires trypsin supplementation to activate its S protein for membrane fusion and virus propagation in cell culture. By substitution of a single amino acid in the S protein, we created a recombinant PEDV with an artificial furin protease cleavage site N terminal of the putative fusion peptide (PEDV-SFCS). PEDV-SFCS exhibited trypsin-independent cell-cell fusion and was able to replicate in culture cells independently of trypsin, though to low titer.
| Medienart: |
E-Artikel |
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| Erscheinungsjahr: |
2015 |
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| Erschienen: |
2015 |
| Enthalten in: |
Zur Gesamtaufnahme - volume:89 |
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| Enthalten in: |
Journal of virology - 89(2015), 15 vom: 01. Aug., Seite 8077-81 |
| Sprache: |
Englisch |
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| Beteiligte Personen: |
Li, Wentao [VerfasserIn] |
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| Links: |
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| Themen: |
EC 3.4.21.4 |
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| Anmerkungen: |
Date Completed 07.12.2015 Date Revised 13.11.2018 published: Print-Electronic Citation Status MEDLINE |
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| doi: |
10.1128/JVI.00356-15 |
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| funding: |
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| Förderinstitution / Projekttitel: |
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| PPN (Katalog-ID): |
NLM248996371 |
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| 100 | 1 | |a Li, Wentao |e verfasserin |4 aut | |
| 245 | 1 | 2 | |a A Single Point Mutation Creating a Furin Cleavage Site in the Spike Protein Renders Porcine Epidemic Diarrhea Coronavirus Trypsin Independent for Cell Entry and Fusion |
| 264 | 1 | |c 2015 | |
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| 500 | |a Date Completed 07.12.2015 | ||
| 500 | |a Date Revised 13.11.2018 | ||
| 500 | |a published: Print-Electronic | ||
| 500 | |a Citation Status MEDLINE | ||
| 520 | |a Copyright © 2015, American Society for Microbiology. All Rights Reserved. | ||
| 520 | |a The emerging porcine epidemic diarrhea virus (PEDV) requires trypsin supplementation to activate its S protein for membrane fusion and virus propagation in cell culture. By substitution of a single amino acid in the S protein, we created a recombinant PEDV with an artificial furin protease cleavage site N terminal of the putative fusion peptide (PEDV-SFCS). PEDV-SFCS exhibited trypsin-independent cell-cell fusion and was able to replicate in culture cells independently of trypsin, though to low titer | ||
| 650 | 4 | |a Journal Article | |
| 650 | 4 | |a Research Support, Non-U.S. Gov't | |
| 650 | 7 | |a Spike Glycoprotein, Coronavirus |2 NLM | |
| 650 | 7 | |a Trypsin |2 NLM | |
| 650 | 7 | |a EC 3.4.21.4 |2 NLM | |
| 650 | 7 | |a Furin |2 NLM | |
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| 700 | 1 | |a Wicht, Oliver |e verfasserin |4 aut | |
| 700 | 1 | |a van Kuppeveld, Frank J M |e verfasserin |4 aut | |
| 700 | 1 | |a He, Qigai |e verfasserin |4 aut | |
| 700 | 1 | |a Rottier, Peter J M |e verfasserin |4 aut | |
| 700 | 1 | |a Bosch, Berend-Jan |e verfasserin |4 aut | |
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