Development of influenza A(H7N9) candidate vaccine viruses with improved hemagglutinin antigen yield in eggs
Published 2015. This article is a U.S. Government work and is in the public domain in the USA..
BACKGROUND: The emergence of avian influenza A(H7N9) virus in poultry causing zoonotic human infections was reported on March 31, 2013. Development of A(H7N9) candidate vaccine viruses (CVV) for pandemic preparedness purposes was initiated without delay. Candidate vaccine viruses were derived by reverse genetics using the internal genes of A/Puerto/Rico/8/34 (PR8). The resulting A(H7N9) CVVs needed improvement because they had titers and antigen yields that were suboptimal for vaccine manufacturing in eggs, especially in a pandemic situation.
METHODS: Two CVVs derived by reverse genetics were serially passaged in embryonated eggs to improve the hemagglutinin (HA) antigen yield. The total viral protein and HA antigen yields of six egg-passaged CVVs were determined by the BCA assay and isotope dilution mass spectrometry (IDMS) analysis, respectively. CVVs were antigenically characterized by hemagglutination inhibition (HI) assays with ferret antisera.
RESULTS: Improvement of total viral protein yield was observed for the six egg-passaged CVVs; HA quantification by IDMS indicated approximately a twofold increase in yield of several egg-passaged viruses as compared to that of the parental CVV. Several different amino acid substitutions were identified in the HA of all viruses after serial passage. However, HI tests indicated that the antigenic properties of two CVVs remained unchanged.
CONCLUSIONS: If influenza A(H7N9) viruses were to acquire sustained human-to-human transmissibility, the improved HA yield of the egg-passaged CVVs generated in this study could expedite vaccine manufacturing for pandemic mitigation.
| Medienart: |
E-Artikel |
|---|
| Erscheinungsjahr: |
2015 |
|---|---|
| Erschienen: |
2015 |
| Enthalten in: |
Zur Gesamtaufnahme - volume:9 |
|---|---|
| Enthalten in: |
Influenza and other respiratory viruses - 9(2015), 5 vom: 12. Sept., Seite 263-70 |
| Sprache: |
Englisch |
|---|
| Beteiligte Personen: |
Ridenour, Callie [VerfasserIn] |
|---|
| Links: |
|---|
| Themen: |
Antigen yield |
|---|
| Anmerkungen: |
Date Completed 09.03.2016 Date Revised 13.11.2018 published: Print Citation Status MEDLINE |
|---|
| doi: |
10.1111/irv.12322 |
|---|
| funding: |
|
|---|---|
| Förderinstitution / Projekttitel: |
|
| PPN (Katalog-ID): |
NLM248898213 |
|---|
| LEADER | 01000naa a22002652 4500 | ||
|---|---|---|---|
| 001 | NLM248898213 | ||
| 003 | DE-627 | ||
| 005 | 20231224152412.0 | ||
| 007 | cr uuu---uuuuu | ||
| 008 | 231224s2015 xx |||||o 00| ||eng c | ||
| 024 | 7 | |a 10.1111/irv.12322 |2 doi | |
| 028 | 5 | 2 | |a pubmed24n0829.xml |
| 035 | |a (DE-627)NLM248898213 | ||
| 035 | |a (NLM)25962412 | ||
| 040 | |a DE-627 |b ger |c DE-627 |e rakwb | ||
| 041 | |a eng | ||
| 100 | 1 | |a Ridenour, Callie |e verfasserin |4 aut | |
| 245 | 1 | 0 | |a Development of influenza A(H7N9) candidate vaccine viruses with improved hemagglutinin antigen yield in eggs |
| 264 | 1 | |c 2015 | |
| 336 | |a Text |b txt |2 rdacontent | ||
| 337 | |a ƒaComputermedien |b c |2 rdamedia | ||
| 338 | |a ƒa Online-Ressource |b cr |2 rdacarrier | ||
| 500 | |a Date Completed 09.03.2016 | ||
| 500 | |a Date Revised 13.11.2018 | ||
| 500 | |a published: Print | ||
| 500 | |a Citation Status MEDLINE | ||
| 520 | |a Published 2015. This article is a U.S. Government work and is in the public domain in the USA. | ||
| 520 | |a BACKGROUND: The emergence of avian influenza A(H7N9) virus in poultry causing zoonotic human infections was reported on March 31, 2013. Development of A(H7N9) candidate vaccine viruses (CVV) for pandemic preparedness purposes was initiated without delay. Candidate vaccine viruses were derived by reverse genetics using the internal genes of A/Puerto/Rico/8/34 (PR8). The resulting A(H7N9) CVVs needed improvement because they had titers and antigen yields that were suboptimal for vaccine manufacturing in eggs, especially in a pandemic situation | ||
| 520 | |a METHODS: Two CVVs derived by reverse genetics were serially passaged in embryonated eggs to improve the hemagglutinin (HA) antigen yield. The total viral protein and HA antigen yields of six egg-passaged CVVs were determined by the BCA assay and isotope dilution mass spectrometry (IDMS) analysis, respectively. CVVs were antigenically characterized by hemagglutination inhibition (HI) assays with ferret antisera | ||
| 520 | |a RESULTS: Improvement of total viral protein yield was observed for the six egg-passaged CVVs; HA quantification by IDMS indicated approximately a twofold increase in yield of several egg-passaged viruses as compared to that of the parental CVV. Several different amino acid substitutions were identified in the HA of all viruses after serial passage. However, HI tests indicated that the antigenic properties of two CVVs remained unchanged | ||
| 520 | |a CONCLUSIONS: If influenza A(H7N9) viruses were to acquire sustained human-to-human transmissibility, the improved HA yield of the egg-passaged CVVs generated in this study could expedite vaccine manufacturing for pandemic mitigation | ||
| 650 | 4 | |a Journal Article | |
| 650 | 4 | |a Research Support, Non-U.S. Gov't | |
| 650 | 4 | |a Antigen yield | |
| 650 | 4 | |a H7N9 | |
| 650 | 4 | |a hemagglutinin | |
| 650 | 4 | |a influenza | |
| 650 | 4 | |a serial passage | |
| 650 | 4 | |a vaccine | |
| 650 | 7 | |a Hemagglutinin Glycoproteins, Influenza Virus |2 NLM | |
| 650 | 7 | |a Influenza Vaccines |2 NLM | |
| 700 | 1 | |a Johnson, Adam |e verfasserin |4 aut | |
| 700 | 1 | |a Winne, Emily |e verfasserin |4 aut | |
| 700 | 1 | |a Hossain, Jaber |e verfasserin |4 aut | |
| 700 | 1 | |a Mateu-Petit, Guaniri |e verfasserin |4 aut | |
| 700 | 1 | |a Balish, Amanda |e verfasserin |4 aut | |
| 700 | 1 | |a Santana, Wanda |e verfasserin |4 aut | |
| 700 | 1 | |a Kim, Taejoong |e verfasserin |4 aut | |
| 700 | 1 | |a Davis, Charles |e verfasserin |4 aut | |
| 700 | 1 | |a Cox, Nancy J |e verfasserin |4 aut | |
| 700 | 1 | |a Barr, John R |e verfasserin |4 aut | |
| 700 | 1 | |a Donis, Ruben O |e verfasserin |4 aut | |
| 700 | 1 | |a Villanueva, Julie |e verfasserin |4 aut | |
| 700 | 1 | |a Williams, Tracie L |e verfasserin |4 aut | |
| 700 | 1 | |a Chen, Li-Mei |e verfasserin |4 aut | |
| 773 | 0 | 8 | |i Enthalten in |t Influenza and other respiratory viruses |d 2007 |g 9(2015), 5 vom: 12. Sept., Seite 263-70 |w (DE-627)NLM176605215 |x 1750-2659 |7 nnns |
| 773 | 1 | 8 | |g volume:9 |g year:2015 |g number:5 |g day:12 |g month:09 |g pages:263-70 |
| 856 | 4 | 0 | |u http://dx.doi.org/10.1111/irv.12322 |3 Volltext |
| 912 | |a GBV_USEFLAG_A | ||
| 912 | |a GBV_NLM | ||
| 951 | |a AR | ||
| 952 | |d 9 |j 2015 |e 5 |b 12 |c 09 |h 263-70 | ||