Enhanced Shewanella biofilm promotes bioelectricity generation
© 2015 Wiley Periodicals, Inc..
Electroactive biofilms play essential roles in determining the power output of microbial fuel cells (MFCs). To engineer the electroactive biofilm formation of Shewanella oneidensis MR-1, a model exoelectrogen, we herein heterologously overexpressed a c-di-GMP biosynthesis gene ydeH in S. oneidensis MR-1, constructing a mutant strain in which the expression of ydeH is under the control of IPTG-inducible promoter, and a strain in which ydeH is under the control of a constitutive promoter. Such engineered Shewanella strains had significantly enhanced biofilm formation and bioelectricity generation. The MFCs inoculated with these engineered strains accomplished a maximum power density of 167.6 ± 3.6 mW/m(2) , which was ∼ 2.8 times of that achieved by the wild-type MR-1 (61.0 ± 1.9 mW/m(2) ). In addition, the engineered strains in the bioelectrochemical system at poised potential of 0.2 V vs. saturated calomel electrode (SCE) generated a stable current density of 1100 mA/m(2) , ∼ 3.4 times of that by wild-type MR-1 (320 mA/m(2) ).
| Medienart: |
E-Artikel |
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| Erscheinungsjahr: |
2015 |
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| Erschienen: |
2015 |
| Enthalten in: |
Zur Gesamtaufnahme - volume:112 |
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| Enthalten in: |
Biotechnology and bioengineering - 112(2015), 10 vom: 03. Okt., Seite 2051-9 |
| Sprache: |
Englisch |
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| Beteiligte Personen: |
Liu, Ting [VerfasserIn] |
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| Links: |
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| Anmerkungen: |
Date Completed 16.05.2016 Date Revised 26.08.2015 published: Print-Electronic Citation Status MEDLINE |
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| doi: |
10.1002/bit.25624 |
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| funding: |
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| Förderinstitution / Projekttitel: |
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NLM248304283 |
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| 520 | |a Electroactive biofilms play essential roles in determining the power output of microbial fuel cells (MFCs). To engineer the electroactive biofilm formation of Shewanella oneidensis MR-1, a model exoelectrogen, we herein heterologously overexpressed a c-di-GMP biosynthesis gene ydeH in S. oneidensis MR-1, constructing a mutant strain in which the expression of ydeH is under the control of IPTG-inducible promoter, and a strain in which ydeH is under the control of a constitutive promoter. Such engineered Shewanella strains had significantly enhanced biofilm formation and bioelectricity generation. The MFCs inoculated with these engineered strains accomplished a maximum power density of 167.6 ± 3.6 mW/m(2) , which was ∼ 2.8 times of that achieved by the wild-type MR-1 (61.0 ± 1.9 mW/m(2) ). In addition, the engineered strains in the bioelectrochemical system at poised potential of 0.2 V vs. saturated calomel electrode (SCE) generated a stable current density of 1100 mA/m(2) , ∼ 3.4 times of that by wild-type MR-1 (320 mA/m(2) ) | ||
| 650 | 4 | |a Journal Article | |
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| 700 | 1 | |a Yu, Yang-Yang |e verfasserin |4 aut | |
| 700 | 1 | |a Deng, Xiao-Peng |e verfasserin |4 aut | |
| 700 | 1 | |a Ng, Chun Kiat |e verfasserin |4 aut | |
| 700 | 1 | |a Cao, Bin |e verfasserin |4 aut | |
| 700 | 1 | |a Wang, Jing-Yuan |e verfasserin |4 aut | |
| 700 | 1 | |a Rice, Scott A |e verfasserin |4 aut | |
| 700 | 1 | |a Kjelleberg, Staffan |e verfasserin |4 aut | |
| 700 | 1 | |a Song, Hao |e verfasserin |4 aut | |
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