Study on fluorescence labeling and determination of polypeptide (PC2~PC6) by high performance liquid chromatography
This study was based on the thiol groups (-SH) of PC2~PC6, which could be reacted with the Monobromobimane (mBBr), in order to get polypeptide derivatives with fluorescent signal. A new method was developed for measuring the Polypeptides by high performance liquid chromatography with fluorescence detector, then the chromatographic conditions of HPLC was optimized; meawhile the reaction proportion of PCs and mBBr was identified by Trap-MS. The results showed that, the reaction proportion of PCs and mBBr was 1:1, the polypeptide derivatives had good stability; the five compounds separation was better, and the peak time focused on the 16.6~22.0 min; the linear correlation coefficient of PC2, PC3, PC4, PC5 and PC6 was >0.9991, and the limits of quantification were 0.3, 0.05, 0.3, 0.5 and 0.8 mg · L(-1) respectively, the recovery rate was 83.0%-102.0%; the method was reproducible, RSD<2%, this method for measuring the peptide compounds was rapid and accurate.
| Medienart: |
Artikel |
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| Erscheinungsjahr: |
2014 |
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| Erschienen: |
2014 |
| Enthalten in: |
Zur Gesamtaufnahme - volume:34 |
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| Enthalten in: |
Guang pu xue yu guang pu fen xi = Guang pu - 34(2014), 12 vom: 13. Dez., Seite 3301-6 |
| Sprache: |
Chinesisch |
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| Beteiligte Personen: |
Li, Jing-xi [VerfasserIn] |
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| Themen: |
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| Anmerkungen: |
Date Completed 17.08.2015 Date Revised 02.12.2018 published: Print Citation Status MEDLINE |
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| Förderinstitution / Projekttitel: |
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| PPN (Katalog-ID): |
NLM248125125 |
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| 245 | 1 | 0 | |a Study on fluorescence labeling and determination of polypeptide (PC2~PC6) by high performance liquid chromatography |
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| 500 | |a Date Revised 02.12.2018 | ||
| 500 | |a published: Print | ||
| 500 | |a Citation Status MEDLINE | ||
| 520 | |a This study was based on the thiol groups (-SH) of PC2~PC6, which could be reacted with the Monobromobimane (mBBr), in order to get polypeptide derivatives with fluorescent signal. A new method was developed for measuring the Polypeptides by high performance liquid chromatography with fluorescence detector, then the chromatographic conditions of HPLC was optimized; meawhile the reaction proportion of PCs and mBBr was identified by Trap-MS. The results showed that, the reaction proportion of PCs and mBBr was 1:1, the polypeptide derivatives had good stability; the five compounds separation was better, and the peak time focused on the 16.6~22.0 min; the linear correlation coefficient of PC2, PC3, PC4, PC5 and PC6 was >0.9991, and the limits of quantification were 0.3, 0.05, 0.3, 0.5 and 0.8 mg · L(-1) respectively, the recovery rate was 83.0%-102.0%; the method was reproducible, RSD<2%, this method for measuring the peptide compounds was rapid and accurate | ||
| 650 | 4 | |a Journal Article | |
| 650 | 7 | |a Peptides |2 NLM | |
| 650 | 7 | |a Sulfhydryl Compounds |2 NLM | |
| 700 | 1 | |a Gao, Li-jie |e verfasserin |4 aut | |
| 700 | 1 | |a Cao, Wei |e verfasserin |4 aut | |
| 700 | 1 | |a Zheng, Li |e verfasserin |4 aut | |
| 700 | 1 | |a Chen, Jun-hui |e verfasserin |4 aut | |
| 700 | 1 | |a Xu, Xiu-li |e verfasserin |4 aut | |
| 700 | 1 | |a Wang, Xiao-ru |e verfasserin |4 aut | |
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