Unlocking the diversity of alkaloids in Catharanthus roseus : nuclear localization suggests metabolic channeling in secondary metabolism
Copyright © 2015 The Authors. Published by Elsevier Ltd.. All rights reserved..
The extraordinary chemical diversity of the plant-derived monoterpene indole alkaloids, which include vinblastine, quinine, and strychnine, originates from a single biosynthetic intermediate, strictosidine aglycone. Here we report for the first time the cloning of a biosynthetic gene and characterization of the corresponding enzyme that acts at this crucial branchpoint. This enzyme, an alcohol dehydrogenase homolog, converts strictosidine aglycone to the heteroyohimbine-type alkaloid tetrahydroalstonine. We also demonstrate how this enzyme, which uses a highly reactive substrate, may interact with the upstream enzyme of the pathway.
| Medienart: |
E-Artikel |
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| Erscheinungsjahr: |
2015 |
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| Erschienen: |
2015 |
| Enthalten in: |
Zur Gesamtaufnahme - volume:22 |
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| Enthalten in: |
Chemistry & biology - 22(2015), 3 vom: 19. März, Seite 336-41 |
| Sprache: |
Englisch |
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| Beteiligte Personen: |
Stavrinides, Anna [VerfasserIn] |
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| Links: |
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| Themen: |
20824-29-7 |
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| Anmerkungen: |
Date Completed 25.02.2016 Date Revised 16.07.2022 published: Print-Electronic Citation Status MEDLINE |
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| doi: |
10.1016/j.chembiol.2015.02.006 |
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| funding: |
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| Förderinstitution / Projekttitel: |
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NLM247096822 |
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| 520 | |a Copyright © 2015 The Authors. Published by Elsevier Ltd.. All rights reserved. | ||
| 520 | |a The extraordinary chemical diversity of the plant-derived monoterpene indole alkaloids, which include vinblastine, quinine, and strychnine, originates from a single biosynthetic intermediate, strictosidine aglycone. Here we report for the first time the cloning of a biosynthetic gene and characterization of the corresponding enzyme that acts at this crucial branchpoint. This enzyme, an alcohol dehydrogenase homolog, converts strictosidine aglycone to the heteroyohimbine-type alkaloid tetrahydroalstonine. We also demonstrate how this enzyme, which uses a highly reactive substrate, may interact with the upstream enzyme of the pathway | ||
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| 700 | 1 | |a Tatsis, Evangelos C |e verfasserin |4 aut | |
| 700 | 1 | |a Foureau, Emilien |e verfasserin |4 aut | |
| 700 | 1 | |a Caputi, Lorenzo |e verfasserin |4 aut | |
| 700 | 1 | |a Kellner, Franziska |e verfasserin |4 aut | |
| 700 | 1 | |a Courdavault, Vincent |e verfasserin |4 aut | |
| 700 | 1 | |a O'Connor, Sarah E |e verfasserin |4 aut | |
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