Molecular Diagnosis of Shrimp Allergy : Efficiency of Several Allergens to Predict Clinical Reactivity
Copyright © 2015 American Academy of Allergy, Asthma & Immunology. Published by Elsevier Inc. All rights reserved..
BACKGROUND: The diagnosis of shellfish allergy remains a challenge for clinicians. Several shellfish allergens have been characterized and their IgE epitopes identified. However, the clinical relevance of this sensitization is still not clear.
OBJECTIVE: The objective of this study was to identify allergens and epitopes associated with clinical reactivity to shrimp.
METHODS: Shrimp-sensitized subjects were recruited and grouped based on the history of shrimp-allergic reactions and challenge outcome. IgE reactivity to recombinant crustacean allergens, and IgE and IgG4 reactivity to peptides were determined. Subjects sensitized to dust mites and/or cockroach without shrimp sensitization or reported allergic reactions, as well as nonatopic individuals, were used as controls.
RESULTS: A total of 86 subjects were recruited with a skin prick test to shrimp; 74 reported shrimp-allergic reactions, 58 were allergic (38 positive double-blind placebo-controlled food challenge and 20 recent anaphylaxis), and 16 were tolerant. All subjects without a history of reactions had negative challenges. The individuals with a positive challenge more frequently recognized tropomyosin and sarcoplasmic calcium-binding proteins than those found tolerant by the challenge. Especially a sarcoplasmic-calcium-binding-protein positive test is very likely to result in a positive challenge, though the frequency of recognition is low. Subjects with dust mite and/or cockroach allergy not sensitized to shrimp recognized arginine kinase and hemocyanin. Several epitopes of these allergens may be important in predicting clinical reactivity.
CONCLUSION: Tropomyosin and sarcoplasmic-calcium-binding-protein sensitization is associated with clinical reactivity to shrimp. Myosin light chain testing may help in the diagnosis of clinical reactivity. Arginine kinase and hemocyanin appear to be cross-reacting allergens between shrimp and arthropods. Detection of IgE to these allergens and some of their epitopes may be better diagnostic tools in the routine workup of shrimp allergy.
| Errataetall: |
CommentIn: J Allergy Clin Immunol Pract. 2015 Jul-Aug;3(4):530-1. - PMID 26164576 |
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| Medienart: |
E-Artikel |
| Erscheinungsjahr: |
2015 |
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| Erschienen: |
2015 |
| Enthalten in: |
Zur Gesamtaufnahme - volume:3 |
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| Enthalten in: |
The journal of allergy and clinical immunology. In practice - 3(2015), 4 vom: 24. Juli, Seite 521-9.e10 |
| Sprache: |
Englisch |
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| Beteiligte Personen: |
Pascal, Mariona [VerfasserIn] |
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| Links: |
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| Anmerkungen: |
Date Completed 07.04.2016 Date Revised 17.03.2022 published: Print-Electronic CommentIn: J Allergy Clin Immunol Pract. 2015 Jul-Aug;3(4):530-1. - PMID 26164576 Citation Status MEDLINE |
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| doi: |
10.1016/j.jaip.2015.02.001 |
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| funding: |
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| Förderinstitution / Projekttitel: |
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| PPN (Katalog-ID): |
NLM247071676 |
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| 500 | |a CommentIn: J Allergy Clin Immunol Pract. 2015 Jul-Aug;3(4):530-1. - PMID 26164576 | ||
| 500 | |a Citation Status MEDLINE | ||
| 520 | |a Copyright © 2015 American Academy of Allergy, Asthma & Immunology. Published by Elsevier Inc. All rights reserved. | ||
| 520 | |a BACKGROUND: The diagnosis of shellfish allergy remains a challenge for clinicians. Several shellfish allergens have been characterized and their IgE epitopes identified. However, the clinical relevance of this sensitization is still not clear | ||
| 520 | |a OBJECTIVE: The objective of this study was to identify allergens and epitopes associated with clinical reactivity to shrimp | ||
| 520 | |a METHODS: Shrimp-sensitized subjects were recruited and grouped based on the history of shrimp-allergic reactions and challenge outcome. IgE reactivity to recombinant crustacean allergens, and IgE and IgG4 reactivity to peptides were determined. Subjects sensitized to dust mites and/or cockroach without shrimp sensitization or reported allergic reactions, as well as nonatopic individuals, were used as controls | ||
| 520 | |a RESULTS: A total of 86 subjects were recruited with a skin prick test to shrimp; 74 reported shrimp-allergic reactions, 58 were allergic (38 positive double-blind placebo-controlled food challenge and 20 recent anaphylaxis), and 16 were tolerant. All subjects without a history of reactions had negative challenges. The individuals with a positive challenge more frequently recognized tropomyosin and sarcoplasmic calcium-binding proteins than those found tolerant by the challenge. Especially a sarcoplasmic-calcium-binding-protein positive test is very likely to result in a positive challenge, though the frequency of recognition is low. Subjects with dust mite and/or cockroach allergy not sensitized to shrimp recognized arginine kinase and hemocyanin. Several epitopes of these allergens may be important in predicting clinical reactivity | ||
| 520 | |a CONCLUSION: Tropomyosin and sarcoplasmic-calcium-binding-protein sensitization is associated with clinical reactivity to shrimp. Myosin light chain testing may help in the diagnosis of clinical reactivity. Arginine kinase and hemocyanin appear to be cross-reacting allergens between shrimp and arthropods. Detection of IgE to these allergens and some of their epitopes may be better diagnostic tools in the routine workup of shrimp allergy | ||
| 650 | 4 | |a Controlled Clinical Trial | |
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| 700 | 1 | |a Yang, Ariana C |e verfasserin |4 aut | |
| 700 | 1 | |a Sánchez-García, Silvia |e verfasserin |4 aut | |
| 700 | 1 | |a Lin, Jing |e verfasserin |4 aut | |
| 700 | 1 | |a Towle, David |e verfasserin |4 aut | |
| 700 | 1 | |a Ibañez, Maria Dolores |e verfasserin |4 aut | |
| 700 | 1 | |a Sastre, Joaquín |e verfasserin |4 aut | |
| 700 | 1 | |a Sampson, Hugh A |e verfasserin |4 aut | |
| 700 | 1 | |a Ayuso, Rosalia |e verfasserin |4 aut | |
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