SOCS3 silencing attenuates eosinophil functions in asthma patients
Eosinophils are one of the key inflammatory cells in asthma. Eosinophils can exert a wide variety of actions through expression and secretion of multiple molecules. Previously, we have demonstrated that eosinophils purified from peripheral blood from asthma patients express high levels of suppressor of cytokine signaling 3 (SOCS3). In this article, SOCS3 gene silencing in eosinophils from asthmatics has been carried out to achieve a better understanding of the suppressor function in eosinophils. SOCS3 siRNA treatment drastically reduced SOCS3 expression in eosinophils, leading to an inhibition of the regulatory transcription factors GATA-3 and FoxP3, also interleukin (IL)-10; in turn, an increased STAT3 phosphorilation was observed. Moreover, SOCS3 abrogation in eosinophils produced impaired migration, adhesion and degranulation. Therefore, SOCS3 might be regarded as an important regulator implicated in eosinophil mobilization from the bone marrow to the lungs during the asthmatic process.
| Medienart: |
E-Artikel |
|---|
| Erscheinungsjahr: |
2015 |
|---|---|
| Erschienen: |
2015 |
| Enthalten in: |
Zur Gesamtaufnahme - volume:16 |
|---|---|
| Enthalten in: |
International journal of molecular sciences - 16(2015), 3 vom: 10. März, Seite 5434-51 |
| Sprache: |
Englisch |
|---|
| Beteiligte Personen: |
Zafra, Ma Paz [VerfasserIn] |
|---|
| Links: |
|---|
| Anmerkungen: |
Date Completed 25.11.2015 Date Revised 13.11.2018 published: Electronic Citation Status MEDLINE |
|---|
| doi: |
10.3390/ijms16035434 |
|---|
| funding: |
|
|---|---|
| Förderinstitution / Projekttitel: |
|
| PPN (Katalog-ID): |
NLM24701608X |
|---|
| LEADER | 01000naa a22002652 4500 | ||
|---|---|---|---|
| 001 | NLM24701608X | ||
| 003 | DE-627 | ||
| 005 | 20231224144317.0 | ||
| 007 | cr uuu---uuuuu | ||
| 008 | 231224s2015 xx |||||o 00| ||eng c | ||
| 024 | 7 | |a 10.3390/ijms16035434 |2 doi | |
| 028 | 5 | 2 | |a pubmed24n0823.xml |
| 035 | |a (DE-627)NLM24701608X | ||
| 035 | |a (NLM)25764157 | ||
| 040 | |a DE-627 |b ger |c DE-627 |e rakwb | ||
| 041 | |a eng | ||
| 100 | 1 | |a Zafra, Ma Paz |e verfasserin |4 aut | |
| 245 | 1 | 0 | |a SOCS3 silencing attenuates eosinophil functions in asthma patients |
| 264 | 1 | |c 2015 | |
| 336 | |a Text |b txt |2 rdacontent | ||
| 337 | |a ƒaComputermedien |b c |2 rdamedia | ||
| 338 | |a ƒa Online-Ressource |b cr |2 rdacarrier | ||
| 500 | |a Date Completed 25.11.2015 | ||
| 500 | |a Date Revised 13.11.2018 | ||
| 500 | |a published: Electronic | ||
| 500 | |a Citation Status MEDLINE | ||
| 520 | |a Eosinophils are one of the key inflammatory cells in asthma. Eosinophils can exert a wide variety of actions through expression and secretion of multiple molecules. Previously, we have demonstrated that eosinophils purified from peripheral blood from asthma patients express high levels of suppressor of cytokine signaling 3 (SOCS3). In this article, SOCS3 gene silencing in eosinophils from asthmatics has been carried out to achieve a better understanding of the suppressor function in eosinophils. SOCS3 siRNA treatment drastically reduced SOCS3 expression in eosinophils, leading to an inhibition of the regulatory transcription factors GATA-3 and FoxP3, also interleukin (IL)-10; in turn, an increased STAT3 phosphorilation was observed. Moreover, SOCS3 abrogation in eosinophils produced impaired migration, adhesion and degranulation. Therefore, SOCS3 might be regarded as an important regulator implicated in eosinophil mobilization from the bone marrow to the lungs during the asthmatic process | ||
| 650 | 4 | |a Journal Article | |
| 650 | 4 | |a Research Support, Non-U.S. Gov't | |
| 650 | 7 | |a FOXP3 protein, human |2 NLM | |
| 650 | 7 | |a Forkhead Transcription Factors |2 NLM | |
| 650 | 7 | |a GATA3 Transcription Factor |2 NLM | |
| 650 | 7 | |a SOCS3 protein, human |2 NLM | |
| 650 | 7 | |a STAT3 Transcription Factor |2 NLM | |
| 650 | 7 | |a Suppressor of Cytokine Signaling 3 Protein |2 NLM | |
| 650 | 7 | |a Suppressor of Cytokine Signaling Proteins |2 NLM | |
| 650 | 7 | |a Interleukin-10 |2 NLM | |
| 650 | 7 | |a 130068-27-8 |2 NLM | |
| 700 | 1 | |a Cañas, Jose A |e verfasserin |4 aut | |
| 700 | 1 | |a Mazzeo, Carla |e verfasserin |4 aut | |
| 700 | 1 | |a Gámez, Cristina |e verfasserin |4 aut | |
| 700 | 1 | |a Sanz, Veronica |e verfasserin |4 aut | |
| 700 | 1 | |a Fernández-Nieto, Mar |e verfasserin |4 aut | |
| 700 | 1 | |a Quirce, Santiago |e verfasserin |4 aut | |
| 700 | 1 | |a Barranco, Pilar |e verfasserin |4 aut | |
| 700 | 1 | |a Ruiz-Hornillos, Javier |e verfasserin |4 aut | |
| 700 | 1 | |a Sastre, Joaquín |e verfasserin |4 aut | |
| 700 | 1 | |a del Pozo, Victoria |e verfasserin |4 aut | |
| 773 | 0 | 8 | |i Enthalten in |t International journal of molecular sciences |d 2008 |g 16(2015), 3 vom: 10. März, Seite 5434-51 |w (DE-627)NLM185552161 |x 1422-0067 |7 nnns |
| 773 | 1 | 8 | |g volume:16 |g year:2015 |g number:3 |g day:10 |g month:03 |g pages:5434-51 |
| 856 | 4 | 0 | |u http://dx.doi.org/10.3390/ijms16035434 |3 Volltext |
| 912 | |a GBV_USEFLAG_A | ||
| 912 | |a GBV_NLM | ||
| 951 | |a AR | ||
| 952 | |d 16 |j 2015 |e 3 |b 10 |c 03 |h 5434-51 | ||