Genome-guided investigation of plant natural product biosynthesis
© 2015 The Authors The Plant Journal © 2015 John Wiley & Sons Ltd..
The medicinal plant Madagascar periwinkle, Catharanthus roseus (L.) G. Don, produces hundreds of biologically active monoterpene-derived indole alkaloid (MIA) metabolites and is the sole source of the potent, expensive anti-cancer compounds vinblastine and vincristine. Access to a genome sequence would enable insights into the biochemistry, control, and evolution of genes responsible for MIA biosynthesis. However, generation of a near-complete, scaffolded genome is prohibitive to small research communities due to the expense, time, and expertise required. In this study, we generated a genome assembly for C. roseus that provides a near-comprehensive representation of the genic space that revealed the genomic context of key points within the MIA biosynthetic pathway including physically clustered genes, tandem gene duplication, expression sub-functionalization, and putative neo-functionalization. The genome sequence also facilitated high resolution co-expression analyses that revealed three distinct clusters of co-expression within the components of the MIA pathway. Coordinated biosynthesis of precursors and intermediates throughout the pathway appear to be a feature of vinblastine/vincristine biosynthesis. The C. roseus genome also revealed localization of enzyme-rich genic regions and transporters near known biosynthetic enzymes, highlighting how even a draft genome sequence can empower the study of high-value specialized metabolites.
| Errataetall: | |
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| Medienart: |
E-Artikel |
| Erscheinungsjahr: |
2015 |
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| Erschienen: |
2015 |
| Enthalten in: |
Zur Gesamtaufnahme - volume:82 |
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| Enthalten in: |
The Plant journal : for cell and molecular biology - 82(2015), 4 vom: 01. Mai, Seite 680-92 |
| Sprache: |
Englisch |
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| Beteiligte Personen: |
Kellner, Franziska [VerfasserIn] |
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| Links: |
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| Themen: |
5V9KLZ54CY |
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| Anmerkungen: |
Date Completed 01.02.2016 Date Revised 16.07.2022 published: Print-Electronic ErratumIn: Plant J. 2019 May;98(4):760. - PMID 31087735 Citation Status MEDLINE |
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| doi: |
10.1111/tpj.12827 |
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| funding: |
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| Förderinstitution / Projekttitel: |
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NLM246969024 |
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| 520 | |a The medicinal plant Madagascar periwinkle, Catharanthus roseus (L.) G. Don, produces hundreds of biologically active monoterpene-derived indole alkaloid (MIA) metabolites and is the sole source of the potent, expensive anti-cancer compounds vinblastine and vincristine. Access to a genome sequence would enable insights into the biochemistry, control, and evolution of genes responsible for MIA biosynthesis. However, generation of a near-complete, scaffolded genome is prohibitive to small research communities due to the expense, time, and expertise required. In this study, we generated a genome assembly for C. roseus that provides a near-comprehensive representation of the genic space that revealed the genomic context of key points within the MIA biosynthetic pathway including physically clustered genes, tandem gene duplication, expression sub-functionalization, and putative neo-functionalization. The genome sequence also facilitated high resolution co-expression analyses that revealed three distinct clusters of co-expression within the components of the MIA pathway. Coordinated biosynthesis of precursors and intermediates throughout the pathway appear to be a feature of vinblastine/vincristine biosynthesis. The C. roseus genome also revealed localization of enzyme-rich genic regions and transporters near known biosynthetic enzymes, highlighting how even a draft genome sequence can empower the study of high-value specialized metabolites | ||
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| 700 | 1 | |a Kim, Jeongwoon |e verfasserin |4 aut | |
| 700 | 1 | |a Clavijo, Bernardo J |e verfasserin |4 aut | |
| 700 | 1 | |a Hamilton, John P |e verfasserin |4 aut | |
| 700 | 1 | |a Childs, Kevin L |e verfasserin |4 aut | |
| 700 | 1 | |a Vaillancourt, Brieanne |e verfasserin |4 aut | |
| 700 | 1 | |a Cepela, Jason |e verfasserin |4 aut | |
| 700 | 1 | |a Habermann, Marc |e verfasserin |4 aut | |
| 700 | 1 | |a Steuernagel, Burkhard |e verfasserin |4 aut | |
| 700 | 1 | |a Clissold, Leah |e verfasserin |4 aut | |
| 700 | 1 | |a McLay, Kirsten |e verfasserin |4 aut | |
| 700 | 1 | |a Buell, Carol Robin |e verfasserin |4 aut | |
| 700 | 1 | |a O'Connor, Sarah E |e verfasserin |4 aut | |
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