Antirestriction activity of the mercury resistance nonconjugative transposon Tn5053 is controlled by the protease ClpXP
When transformed into Escherichia coli K12 strains, the mercury resistance transposon Tn5053exhibits high antirestriction activity against the EcoKI type I restriction and modification system. The products of the genes merR and ardD contribute to the antirestriction activity of Tn5053. The merR gene encodes the MerR protein, the transcription regulator of the mer operon genes. The ardD gene is responsible for ArdD protein synthesis and is located within the tni operon. In the following study, it was demonstrated that the antirestriction activity of the transposon Tn5053 is absent in E. coli K12 strains with the mutant genes clpX, clpP, and recA. The antirestriction effect of Tn5053 is not enhanced by 2-aminopurine. The Tn5053 antirestriction activity is not altered in E. coli K12 with the mutant dam gene; however, it is decreased in the E. coli K12 mutD. It is assumed that the activities of the MerR and ArdD proteins lead to the formation of a significant amount of unmodified DNA in the bacterial cell, causing the SOS-dependent reduction of the EcoKI (R2M2S) enzyme activity associated with ClpXP-induced proteolysis of the R-subuinit.
| Medienart: |
Artikel |
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| Erscheinungsjahr: |
2014 |
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| Erschienen: |
2014 |
| Enthalten in: |
Zur Gesamtaufnahme - volume:50 |
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| Enthalten in: |
Genetika - 50(2014), 9 vom: 24. Sept., Seite 1033-9 |
| Sprache: |
Russisch |
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| Beteiligte Personen: |
Zavigel'skiĭ, G B [VerfasserIn] |
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| Anmerkungen: |
Date Completed 24.03.2015 Date Revised 04.03.2015 published: Print Citation Status MEDLINE |
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| Förderinstitution / Projekttitel: |
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| PPN (Katalog-ID): |
NLM246735554 |
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| 100 | 1 | |a Zavigel'skiĭ, G B |e verfasserin |4 aut | |
| 245 | 1 | 0 | |a Antirestriction activity of the mercury resistance nonconjugative transposon Tn5053 is controlled by the protease ClpXP |
| 264 | 1 | |c 2014 | |
| 336 | |a Text |b txt |2 rdacontent | ||
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| 500 | |a Date Completed 24.03.2015 | ||
| 500 | |a Date Revised 04.03.2015 | ||
| 500 | |a published: Print | ||
| 500 | |a Citation Status MEDLINE | ||
| 520 | |a When transformed into Escherichia coli K12 strains, the mercury resistance transposon Tn5053exhibits high antirestriction activity against the EcoKI type I restriction and modification system. The products of the genes merR and ardD contribute to the antirestriction activity of Tn5053. The merR gene encodes the MerR protein, the transcription regulator of the mer operon genes. The ardD gene is responsible for ArdD protein synthesis and is located within the tni operon. In the following study, it was demonstrated that the antirestriction activity of the transposon Tn5053 is absent in E. coli K12 strains with the mutant genes clpX, clpP, and recA. The antirestriction effect of Tn5053 is not enhanced by 2-aminopurine. The Tn5053 antirestriction activity is not altered in E. coli K12 with the mutant dam gene; however, it is decreased in the E. coli K12 mutD. It is assumed that the activities of the MerR and ArdD proteins lead to the formation of a significant amount of unmodified DNA in the bacterial cell, causing the SOS-dependent reduction of the EcoKI (R2M2S) enzyme activity associated with ClpXP-induced proteolysis of the R-subuinit | ||
| 650 | 4 | |a English Abstract | |
| 650 | 4 | |a Journal Article | |
| 650 | 4 | |a Research Support, Non-U.S. Gov't | |
| 650 | 7 | |a Bacterial Proteins |2 NLM | |
| 650 | 7 | |a DNA Transposable Elements |2 NLM | |
| 650 | 7 | |a DNA-Binding Proteins |2 NLM | |
| 650 | 7 | |a Escherichia coli Proteins |2 NLM | |
| 650 | 7 | |a MerR protein, Bacteria |2 NLM | |
| 650 | 7 | |a ardD protein, E coli |2 NLM | |
| 650 | 7 | |a Site-Specific DNA-Methyltransferase (Adenine-Specific) |2 NLM | |
| 650 | 7 | |a EC 2.1.1.72 |2 NLM | |
| 650 | 7 | |a dam protein, E coli |2 NLM | |
| 650 | 7 | |a EC 2.1.1.72 |2 NLM | |
| 650 | 7 | |a ClpXP protease, E coli |2 NLM | |
| 650 | 7 | |a EC 3.4.21.92 |2 NLM | |
| 650 | 7 | |a Endopeptidase Clp |2 NLM | |
| 650 | 7 | |a EC 3.4.21.92 |2 NLM | |
| 650 | 7 | |a Mercury |2 NLM | |
| 650 | 7 | |a FXS1BY2PGL |2 NLM | |
| 700 | 1 | |a Kotova, V Iu |e verfasserin |4 aut | |
| 700 | 1 | |a Mel'kina, O E |e verfasserin |4 aut | |
| 700 | 1 | |a Pustovoĭt, K S |e verfasserin |4 aut | |
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