The molecular insight into the antihyperuricemic and renoprotective effect of Shuang Qi gout capsule in mice
Copyright © 2015 Elsevier Ireland Ltd. All rights reserved..
ETHNOPHARMACOLOGICAL RELEVANCE: Shuang-Qi gout capsule, a traditional Chinese medicine prescription, has been used in the treatment of, gout arthritis, arthralgia and inflammation. Since renal urate overload associated with severe disability including gout, elimination of excess renal uric acid is highly essential. Therefore, in this study we evaluated the antihyperuricemic and the renoprotective effect of the Shuang Qi gout capsule (SQ) with elucidation of its mechanism.
MATERIALS AND METHODS: We assessed the antihyperuricemic activity of SQ on urinary and serum uric acid, creatinine, blood urea nitrogen, fractional excretion of uric acid (FEUA) and glomerular filtration rate of creatinine and uric acid in potassium oxonate (PO) - induced mice as well as in non-induced mice. To illuminate the mechanism of antihyperuricemic activity, we investigated renal transport activity and the expression of mRNA levels in PO-induced and non-induced mice by western blot and RT-PCR methods.
RESULTS: SQ showed significant reduction in serum uric acid, creatinine and blood urea nitrogen levels and marked elevation of urine uric acid, creatinine and FEUA levels only in hyperuricemic mice. Furthermore, SQ could recover the altered expressions of proteins and mRNA levels of all the main renal transporters significantly in dose dependent manner.
CONCLUSIONS: SQ could effectively regulate the main renal transporters denoted its denote probable antihyperuricemic mechanism of SQ and its dose dependent uricosuric effect. In addition, SQ attenuated the deleterious effects of hyperuricemia with renal dysfunction. Thus SQ could be a potent antihyperuricemic agent which can perform as a safer and effective agent in the management of hyperuricemia via regulating the renal transporters.
| Medienart: |
E-Artikel |
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| Erscheinungsjahr: |
2015 |
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| Erschienen: |
2015 |
| Enthalten in: |
Zur Gesamtaufnahme - volume:163 |
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| Enthalten in: |
Journal of ethnopharmacology - 163(2015) vom: 02. Apr., Seite 278-89 |
| Sprache: |
Englisch |
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| Beteiligte Personen: |
Kodithuwakku, Nandani Darshika [VerfasserIn] |
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| Links: |
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| Anmerkungen: |
Date Completed 17.12.2015 Date Revised 18.03.2022 published: Print-Electronic Citation Status MEDLINE |
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| doi: |
10.1016/j.jep.2015.01.013 |
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| Förderinstitution / Projekttitel: |
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NLM245586229 |
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| 100 | 1 | |a Kodithuwakku, Nandani Darshika |e verfasserin |4 aut | |
| 245 | 1 | 4 | |a The molecular insight into the antihyperuricemic and renoprotective effect of Shuang Qi gout capsule in mice |
| 264 | 1 | |c 2015 | |
| 336 | |a Text |b txt |2 rdacontent | ||
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| 500 | |a Date Completed 17.12.2015 | ||
| 500 | |a Date Revised 18.03.2022 | ||
| 500 | |a published: Print-Electronic | ||
| 500 | |a Citation Status MEDLINE | ||
| 520 | |a Copyright © 2015 Elsevier Ireland Ltd. All rights reserved. | ||
| 520 | |a ETHNOPHARMACOLOGICAL RELEVANCE: Shuang-Qi gout capsule, a traditional Chinese medicine prescription, has been used in the treatment of, gout arthritis, arthralgia and inflammation. Since renal urate overload associated with severe disability including gout, elimination of excess renal uric acid is highly essential. Therefore, in this study we evaluated the antihyperuricemic and the renoprotective effect of the Shuang Qi gout capsule (SQ) with elucidation of its mechanism | ||
| 520 | |a MATERIALS AND METHODS: We assessed the antihyperuricemic activity of SQ on urinary and serum uric acid, creatinine, blood urea nitrogen, fractional excretion of uric acid (FEUA) and glomerular filtration rate of creatinine and uric acid in potassium oxonate (PO) - induced mice as well as in non-induced mice. To illuminate the mechanism of antihyperuricemic activity, we investigated renal transport activity and the expression of mRNA levels in PO-induced and non-induced mice by western blot and RT-PCR methods | ||
| 520 | |a RESULTS: SQ showed significant reduction in serum uric acid, creatinine and blood urea nitrogen levels and marked elevation of urine uric acid, creatinine and FEUA levels only in hyperuricemic mice. Furthermore, SQ could recover the altered expressions of proteins and mRNA levels of all the main renal transporters significantly in dose dependent manner | ||
| 520 | |a CONCLUSIONS: SQ could effectively regulate the main renal transporters denoted its denote probable antihyperuricemic mechanism of SQ and its dose dependent uricosuric effect. In addition, SQ attenuated the deleterious effects of hyperuricemia with renal dysfunction. Thus SQ could be a potent antihyperuricemic agent which can perform as a safer and effective agent in the management of hyperuricemia via regulating the renal transporters | ||
| 650 | 4 | |a Journal Article | |
| 650 | 4 | |a Antihyperuricemic activity | |
| 650 | 4 | |a Gout | |
| 650 | 4 | |a Mice | |
| 650 | 4 | |a Regulatory actions | |
| 650 | 4 | |a Renal transporters | |
| 650 | 4 | |a SQ gout capsule | |
| 650 | 7 | |a ATP Binding Cassette Transporter, Subfamily G, Member 2 |2 NLM | |
| 650 | 7 | |a ATP-Binding Cassette Transporters |2 NLM | |
| 650 | 7 | |a Abcg2 protein, mouse |2 NLM | |
| 650 | 7 | |a Capsules |2 NLM | |
| 650 | 7 | |a Carrier Proteins |2 NLM | |
| 650 | 7 | |a Drugs, Chinese Herbal |2 NLM | |
| 650 | 7 | |a Gout Suppressants |2 NLM | |
| 650 | 7 | |a Membrane Proteins |2 NLM | |
| 650 | 7 | |a Organic Anion Transporters |2 NLM | |
| 650 | 7 | |a Organic Cation Transport Proteins |2 NLM | |
| 650 | 7 | |a Protective Agents |2 NLM | |
| 650 | 7 | |a Shuang-Qi |2 NLM | |
| 650 | 7 | |a Slc22a12 protein, mouse |2 NLM | |
| 650 | 7 | |a Slc22a4 protein, mouse |2 NLM | |
| 650 | 7 | |a Slc22a5 protein, mouse |2 NLM | |
| 650 | 7 | |a Solute Carrier Family 22 Member 5 |2 NLM | |
| 650 | 7 | |a Symporters |2 NLM | |
| 650 | 7 | |a Uric Acid |2 NLM | |
| 650 | 7 | |a 268B43MJ25 |2 NLM | |
| 650 | 7 | |a potassium oxonate |2 NLM | |
| 650 | 7 | |a 4R7FFA00RX |2 NLM | |
| 650 | 7 | |a Oxonic Acid |2 NLM | |
| 650 | 7 | |a 5VT6420TIG |2 NLM | |
| 650 | 7 | |a Creatinine |2 NLM | |
| 650 | 7 | |a AYI8EX34EU |2 NLM | |
| 700 | 1 | |a Feng, Yi-dong |e verfasserin |4 aut | |
| 700 | 1 | |a Zhang, Yan-yan |e verfasserin |4 aut | |
| 700 | 1 | |a Pan, Min |e verfasserin |4 aut | |
| 700 | 1 | |a Fang, Wei-rong |e verfasserin |4 aut | |
| 700 | 1 | |a Li, Yun-man |e verfasserin |4 aut | |
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| 856 | 4 | 0 | |u http://dx.doi.org/10.1016/j.jep.2015.01.013 |3 Volltext |
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