A unique cis-3-hydroxy-l-proline dehydratase in the enolase superfamily
The genome of Labrenzia aggregata IAM 12614 encodes an uncharacterized member of the muconate lactonizing enzyme (MLE) subgroup of the enolase superfamily (UniProt ID A0NXQ8 ). The gene encoding A0NXQ8 is located between genes that encode members of the proline racemase superfamily, 4R-hydroxyproline 2-epimerase (UniProt ID A0NXQ7 ; 4HypE) and trans-3-hydroxy-l-proline dehydratase (UniProt ID A0NXQ9 ; t3LHypD). A0NXQ8 was screened with a library of proline analogues; two reactions were observed with cis-3-hydroxy-l-proline (c3LHyp), competing 2-epimerization to trans-3-hydroxy-d-proline (1,1-proton transfer) and dehydration to Δ(1)-pyrroline-2-carboxylate (β-elimination; c3LHyp dehydratase), with eventual total dehydration. The genome context encoding A0NXQ8 both (1) confirms its novel c3LHyp dehydratase function and (2) provides evidence for metabolic pathways that allow L. aggregata to utilize several isomeric 3- and 4-hydroxyprolines as sole carbon sources.
| Medienart: |
E-Artikel |
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| Erscheinungsjahr: |
2015 |
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| Erschienen: |
2015 |
| Enthalten in: |
Zur Gesamtaufnahme - volume:137 |
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| Enthalten in: |
Journal of the American Chemical Society - 137(2015), 4 vom: 04. Feb., Seite 1388-91 |
| Sprache: |
Englisch |
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| Beteiligte Personen: |
Zhang, Xinshuai [VerfasserIn] |
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| Links: |
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| Anmerkungen: |
Date Completed 24.09.2015 Date Revised 20.02.2019 published: Print-Electronic Citation Status MEDLINE |
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| doi: |
10.1021/ja5103986 |
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| Förderinstitution / Projekttitel: |
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| PPN (Katalog-ID): |
NLM245532366 |
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| 100 | 1 | |a Zhang, Xinshuai |e verfasserin |4 aut | |
| 245 | 1 | 2 | |a A unique cis-3-hydroxy-l-proline dehydratase in the enolase superfamily |
| 264 | 1 | |c 2015 | |
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| 500 | |a Date Completed 24.09.2015 | ||
| 500 | |a Date Revised 20.02.2019 | ||
| 500 | |a published: Print-Electronic | ||
| 500 | |a Citation Status MEDLINE | ||
| 520 | |a The genome of Labrenzia aggregata IAM 12614 encodes an uncharacterized member of the muconate lactonizing enzyme (MLE) subgroup of the enolase superfamily (UniProt ID A0NXQ8 ). The gene encoding A0NXQ8 is located between genes that encode members of the proline racemase superfamily, 4R-hydroxyproline 2-epimerase (UniProt ID A0NXQ7 ; 4HypE) and trans-3-hydroxy-l-proline dehydratase (UniProt ID A0NXQ9 ; t3LHypD). A0NXQ8 was screened with a library of proline analogues; two reactions were observed with cis-3-hydroxy-l-proline (c3LHyp), competing 2-epimerization to trans-3-hydroxy-d-proline (1,1-proton transfer) and dehydration to Δ(1)-pyrroline-2-carboxylate (β-elimination; c3LHyp dehydratase), with eventual total dehydration. The genome context encoding A0NXQ8 both (1) confirms its novel c3LHyp dehydratase function and (2) provides evidence for metabolic pathways that allow L. aggregata to utilize several isomeric 3- and 4-hydroxyprolines as sole carbon sources | ||
| 650 | 4 | |a Journal Article | |
| 650 | 4 | |a Research Support, N.I.H., Extramural | |
| 650 | 4 | |a Research Support, U.S. Gov't, Non-P.H.S. | |
| 650 | 7 | |a Hydro-Lyases |2 NLM | |
| 650 | 7 | |a EC 4.2.1.- |2 NLM | |
| 650 | 7 | |a Amino Acid Isomerases |2 NLM | |
| 650 | 7 | |a EC 5.1.1.- |2 NLM | |
| 650 | 7 | |a hydroxyproline epimerase |2 NLM | |
| 650 | 7 | |a EC 5.1.1.8 |2 NLM | |
| 650 | 7 | |a Hydroxyproline |2 NLM | |
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| 700 | 1 | |a Kumar, Ritesh |e verfasserin |4 aut | |
| 700 | 1 | |a Vetting, Matthew W |e verfasserin |4 aut | |
| 700 | 1 | |a Zhao, Suwen |e verfasserin |4 aut | |
| 700 | 1 | |a Jacobson, Matthew P |e verfasserin |4 aut | |
| 700 | 1 | |a Almo, Steven C |e verfasserin |4 aut | |
| 700 | 1 | |a Gerlt, John A |e verfasserin |4 aut | |
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