Anti-inflammatory and antioxidant properties of unripe papaya extract in an excision wound model
CONTEXT: Carica papaya L. (Caricaceae) fruit was shown to exhibit wound healing properties.
OBJECTIVES: We investigated anti-inflammatory and antioxidant potential of papaya fruit phosphate-buffered saline extract (PE) during wound healing and enhancement of the potentials due to trace ions addition.
MATERIALS AND METHODS: Rat excision wounds were topically treated twice/day with 20 µL of PE (5 mg extract/mL), 0.5 µg Se(2+) added PE (PES), or 100 µM Zn(2+) added PE (PEZ). Control groups were treated with deionized water (negative) and deproteinized calf blood extract ointment (Solcoseryl®, positive). Lipid peroxidation (LPX), antioxidant, proinflammatory, and arginine metabolic enzymes were estimated in the wound excised on days 4 and 10 post wounding.
RESULTS: PE (5 mg/mL; 9.80 ± 0.33 d) and PES (PE + 0.5 µg Se(2+); 8.90 ± 0.23 d) significantly (p < 0.05) reduced the average time for complete wound closure compared with the negative (13.00 ± 0.37 d) and positive (9.80 ± 0.33 d) controls, respectively. Biochemical evaluations of LPX product (malondialdehyde), antioxidant (catalase, superoxide dismutase (SOD), and glutathione peroxidase (GPx)), and pro-inflammatory (cyclooxygenase-2 and myeloperoxidase (MPO)) enzyme activities and metabolites (nitrite and urea), on days 4 and 10 post wounding, confirmed the anti-inflammatory and antioxidant properties of PE and PES in this study.
DISCUSSION AND CONCLUSION: Treatment of excision wounds with papaya extract, especially with the addition of selenium for 10 d, reduced inflammation associated oxidative damage apparently via cyclooxygenase specific inhibition, arginine metabolism, and up-regulation of antioxidant enzymes.
| Medienart: |
E-Artikel |
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| Erscheinungsjahr: |
2015 |
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| Erschienen: |
2015 |
| Enthalten in: |
Zur Gesamtaufnahme - volume:53 |
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| Enthalten in: |
Pharmaceutical biology - 53(2015), 5 vom: 29. Mai, Seite 662-71 |
| Sprache: |
Englisch |
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| Beteiligte Personen: |
Nafiu, Abdulrazaq Bidemi [VerfasserIn] |
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| Links: |
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| Anmerkungen: |
Date Completed 04.01.2016 Date Revised 11.03.2015 published: Print-Electronic Citation Status MEDLINE |
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| doi: |
10.3109/13880209.2014.936470 |
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| funding: |
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| Förderinstitution / Projekttitel: |
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| PPN (Katalog-ID): |
NLM243863861 |
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| 100 | 1 | |a Nafiu, Abdulrazaq Bidemi |e verfasserin |4 aut | |
| 245 | 1 | 0 | |a Anti-inflammatory and antioxidant properties of unripe papaya extract in an excision wound model |
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| 500 | |a Date Completed 04.01.2016 | ||
| 500 | |a Date Revised 11.03.2015 | ||
| 500 | |a published: Print-Electronic | ||
| 500 | |a Citation Status MEDLINE | ||
| 520 | |a CONTEXT: Carica papaya L. (Caricaceae) fruit was shown to exhibit wound healing properties | ||
| 520 | |a OBJECTIVES: We investigated anti-inflammatory and antioxidant potential of papaya fruit phosphate-buffered saline extract (PE) during wound healing and enhancement of the potentials due to trace ions addition | ||
| 520 | |a MATERIALS AND METHODS: Rat excision wounds were topically treated twice/day with 20 µL of PE (5 mg extract/mL), 0.5 µg Se(2+) added PE (PES), or 100 µM Zn(2+) added PE (PEZ). Control groups were treated with deionized water (negative) and deproteinized calf blood extract ointment (Solcoseryl®, positive). Lipid peroxidation (LPX), antioxidant, proinflammatory, and arginine metabolic enzymes were estimated in the wound excised on days 4 and 10 post wounding | ||
| 520 | |a RESULTS: PE (5 mg/mL; 9.80 ± 0.33 d) and PES (PE + 0.5 µg Se(2+); 8.90 ± 0.23 d) significantly (p < 0.05) reduced the average time for complete wound closure compared with the negative (13.00 ± 0.37 d) and positive (9.80 ± 0.33 d) controls, respectively. Biochemical evaluations of LPX product (malondialdehyde), antioxidant (catalase, superoxide dismutase (SOD), and glutathione peroxidase (GPx)), and pro-inflammatory (cyclooxygenase-2 and myeloperoxidase (MPO)) enzyme activities and metabolites (nitrite and urea), on days 4 and 10 post wounding, confirmed the anti-inflammatory and antioxidant properties of PE and PES in this study | ||
| 520 | |a DISCUSSION AND CONCLUSION: Treatment of excision wounds with papaya extract, especially with the addition of selenium for 10 d, reduced inflammation associated oxidative damage apparently via cyclooxygenase specific inhibition, arginine metabolism, and up-regulation of antioxidant enzymes | ||
| 650 | 4 | |a Journal Article | |
| 650 | 4 | |a Research Support, Non-U.S. Gov't | |
| 650 | 4 | |a Antioxidant enzyme | |
| 650 | 4 | |a arginine metabolism | |
| 650 | 4 | |a lipid peroxidation | |
| 650 | 4 | |a proinflammatory enzymes | |
| 650 | 4 | |a selenium | |
| 650 | 4 | |a wound healing | |
| 650 | 7 | |a Anti-Inflammatory Agents |2 NLM | |
| 650 | 7 | |a Antioxidants |2 NLM | |
| 650 | 7 | |a Plant Extracts |2 NLM | |
| 700 | 1 | |a Rahman, Mohammad Tariqur |e verfasserin |4 aut | |
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