Persistent production of an integrase-deleted HIV-1 variant with no resistance mutation and wild-type proviral DNA in a treated patient
An HIV-infected patient presenting an unexpected viral escape under combined antiretroviral treatment is described. The virus isolated from plasma contained a large deletion in the HIV-1 integrase gene but no known resistance mutation. Nested polymerase chain reactions (PCRs) with patient virus integrase-specific primers and probes were developed and used to detect the mutant from plasma, blood, rectal biopsies, and sperm. The variant progressively emerged during a period of therapy-induced virosuppression, and persisted at a low but detectable level for at least 5 years. Surprisingly, proviral DNA from lymphocytes, rectal cells, and sperm cells was, and remained, mainly wild type. Cellular HIV RNA with the deletion was detected only once from the rectum. The origin and mechanisms underlying this so far not described production at a detectable level are largely hypothetical. This observation raised concern about the ability of defective viruses to spread.
| Medienart: |
E-Artikel |
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| Erscheinungsjahr: |
2015 |
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| Erschienen: |
2015 |
| Enthalten in: |
Zur Gesamtaufnahme - volume:31 |
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| Enthalten in: |
AIDS research and human retroviruses - 31(2015), 1 vom: 20. Jan., Seite 142-9 |
| Sprache: |
Englisch |
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| Beteiligte Personen: |
Trabaud, Mary-Anne [VerfasserIn] |
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| Links: |
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| Themen: |
2494G1JF75 |
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| Anmerkungen: |
Date Completed 11.09.2015 Date Revised 21.10.2021 published: Print GENBANK: KJ173816, KJ173817 Citation Status MEDLINE |
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| doi: |
10.1089/AID.2014.0129 |
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| funding: |
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| Förderinstitution / Projekttitel: |
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| PPN (Katalog-ID): |
NLM242957307 |
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| 245 | 1 | 0 | |a Persistent production of an integrase-deleted HIV-1 variant with no resistance mutation and wild-type proviral DNA in a treated patient |
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| 500 | |a published: Print | ||
| 500 | |a GENBANK: KJ173816, KJ173817 | ||
| 500 | |a Citation Status MEDLINE | ||
| 520 | |a An HIV-infected patient presenting an unexpected viral escape under combined antiretroviral treatment is described. The virus isolated from plasma contained a large deletion in the HIV-1 integrase gene but no known resistance mutation. Nested polymerase chain reactions (PCRs) with patient virus integrase-specific primers and probes were developed and used to detect the mutant from plasma, blood, rectal biopsies, and sperm. The variant progressively emerged during a period of therapy-induced virosuppression, and persisted at a low but detectable level for at least 5 years. Surprisingly, proviral DNA from lymphocytes, rectal cells, and sperm cells was, and remained, mainly wild type. Cellular HIV RNA with the deletion was detected only once from the rectum. The origin and mechanisms underlying this so far not described production at a detectable level are largely hypothetical. This observation raised concern about the ability of defective viruses to spread | ||
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| 700 | 1 | |a Cotte, Laurent |e verfasserin |4 aut | |
| 700 | 1 | |a Saison, Julien |e verfasserin |4 aut | |
| 700 | 1 | |a Ramière, Christophe |e verfasserin |4 aut | |
| 700 | 1 | |a Ronfort, Corinne |e verfasserin |4 aut | |
| 700 | 1 | |a Venet, Fabienne |e verfasserin |4 aut | |
| 700 | 1 | |a Tardy, Jean-Claude |e verfasserin |4 aut | |
| 700 | 1 | |a Monneret, Guillaume |e verfasserin |4 aut | |
| 700 | 1 | |a André, Patrice |e verfasserin |4 aut | |
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