Rod photopigment kinetics after photodisruption of the retinal pigment epithelium
Copyright 2014 The Association for Research in Vision and Ophthalmology, Inc..
PURPOSE: Advances in retinal imaging have led to the discovery of long-lasting retinal changes caused by light exposures below published safety limits, including disruption of the RPE. To investigate the functional consequences of RPE disruption, we combined adaptive optics ophthalmoscopy with retinal densitometry.
METHODS: A modified adaptive optics scanning light ophthalmoscope (AOSLO) measured the apparent density and regeneration rate of rhodopsin in two macaques before and after four different 568-nm retinal radiant exposures (RREs; 400-3200 J/cm(2)). Optical coherence tomography (OCT) was used to measure the optical path length through the photoreceptor outer segments before and after RPE disruption.
RESULTS: All tested RREs caused visible RPE disruption. Apparent rhodopsin density was significantly reduced following 1600 (P = 0.01) and 3200 J/cm(2) (P = 0.007) exposures. No significant change in apparent density was observed in response to 800 J/cm(2). Surprisingly, exposure to 400 J/cm(2) showed a significant increase in apparent density (P = 0.047). Rhodopsin recovery rate was not significantly affected by these RREs. Optical coherence tomography measurements showed a significant decrease in the optical path length through the photoreceptor outer segments for RREs above 800 J/cm(2) (P < 0.001).
CONCLUSIONS: At higher RREs, optical path length through the outer segments was reduced. However, the rate of photopigment regeneration was unchanged. While some ambiguity remains as to the correlation between measured reflectivity and absolute rhodopsin density; at the lowest RREs, RPE disruption appears not to be accompanied by a loss of apparent rhodopsin density, which would have been indicative of functional loss.
| Medienart: |
E-Artikel |
|---|
| Erscheinungsjahr: |
2014 |
|---|---|
| Erschienen: |
2014 |
| Enthalten in: |
Zur Gesamtaufnahme - volume:55 |
|---|---|
| Enthalten in: |
Investigative ophthalmology & visual science - 55(2014), 11 vom: 14. Okt., Seite 7535-44 |
| Sprache: |
Englisch |
|---|
| Beteiligte Personen: |
Masella, Benjamin D [VerfasserIn] |
|---|
| Links: |
|---|
| Themen: |
9009-81-8 |
|---|
| Anmerkungen: |
Date Completed 27.01.2015 Date Revised 21.10.2021 published: Electronic Citation Status MEDLINE |
|---|
| doi: |
10.1167/iovs.13-13796 |
|---|
| funding: |
|
|---|---|
| Förderinstitution / Projekttitel: |
|
| PPN (Katalog-ID): |
NLM242796257 |
|---|
| LEADER | 01000naa a22002652 4500 | ||
|---|---|---|---|
| 001 | NLM242796257 | ||
| 003 | DE-627 | ||
| 005 | 20231224131152.0 | ||
| 007 | cr uuu---uuuuu | ||
| 008 | 231224s2014 xx |||||o 00| ||eng c | ||
| 024 | 7 | |a 10.1167/iovs.13-13796 |2 doi | |
| 028 | 5 | 2 | |a pubmed24n0809.xml |
| 035 | |a (DE-627)NLM242796257 | ||
| 035 | |a (NLM)25316724 | ||
| 040 | |a DE-627 |b ger |c DE-627 |e rakwb | ||
| 041 | |a eng | ||
| 100 | 1 | |a Masella, Benjamin D |e verfasserin |4 aut | |
| 245 | 1 | 0 | |a Rod photopigment kinetics after photodisruption of the retinal pigment epithelium |
| 264 | 1 | |c 2014 | |
| 336 | |a Text |b txt |2 rdacontent | ||
| 337 | |a ƒaComputermedien |b c |2 rdamedia | ||
| 338 | |a ƒa Online-Ressource |b cr |2 rdacarrier | ||
| 500 | |a Date Completed 27.01.2015 | ||
| 500 | |a Date Revised 21.10.2021 | ||
| 500 | |a published: Electronic | ||
| 500 | |a Citation Status MEDLINE | ||
| 520 | |a Copyright 2014 The Association for Research in Vision and Ophthalmology, Inc. | ||
| 520 | |a PURPOSE: Advances in retinal imaging have led to the discovery of long-lasting retinal changes caused by light exposures below published safety limits, including disruption of the RPE. To investigate the functional consequences of RPE disruption, we combined adaptive optics ophthalmoscopy with retinal densitometry | ||
| 520 | |a METHODS: A modified adaptive optics scanning light ophthalmoscope (AOSLO) measured the apparent density and regeneration rate of rhodopsin in two macaques before and after four different 568-nm retinal radiant exposures (RREs; 400-3200 J/cm(2)). Optical coherence tomography (OCT) was used to measure the optical path length through the photoreceptor outer segments before and after RPE disruption | ||
| 520 | |a RESULTS: All tested RREs caused visible RPE disruption. Apparent rhodopsin density was significantly reduced following 1600 (P = 0.01) and 3200 J/cm(2) (P = 0.007) exposures. No significant change in apparent density was observed in response to 800 J/cm(2). Surprisingly, exposure to 400 J/cm(2) showed a significant increase in apparent density (P = 0.047). Rhodopsin recovery rate was not significantly affected by these RREs. Optical coherence tomography measurements showed a significant decrease in the optical path length through the photoreceptor outer segments for RREs above 800 J/cm(2) (P < 0.001) | ||
| 520 | |a CONCLUSIONS: At higher RREs, optical path length through the outer segments was reduced. However, the rate of photopigment regeneration was unchanged. While some ambiguity remains as to the correlation between measured reflectivity and absolute rhodopsin density; at the lowest RREs, RPE disruption appears not to be accompanied by a loss of apparent rhodopsin density, which would have been indicative of functional loss | ||
| 650 | 4 | |a Journal Article | |
| 650 | 4 | |a Research Support, N.I.H., Extramural | |
| 650 | 4 | |a Research Support, Non-U.S. Gov't | |
| 650 | 4 | |a dark adaptation | |
| 650 | 4 | |a densitometry | |
| 650 | 4 | |a light damage | |
| 650 | 4 | |a retinal pigment epithelium | |
| 650 | 4 | |a rhodopsin regeneration | |
| 650 | 7 | |a Rhodopsin |2 NLM | |
| 650 | 7 | |a 9009-81-8 |2 NLM | |
| 700 | 1 | |a Hunter, Jennifer J |e verfasserin |4 aut | |
| 700 | 1 | |a Williams, David R |e verfasserin |4 aut | |
| 773 | 0 | 8 | |i Enthalten in |t Investigative ophthalmology & visual science |d 1985 |g 55(2014), 11 vom: 14. Okt., Seite 7535-44 |w (DE-627)NLM000138320 |x 1552-5783 |7 nnns |
| 773 | 1 | 8 | |g volume:55 |g year:2014 |g number:11 |g day:14 |g month:10 |g pages:7535-44 |
| 856 | 4 | 0 | |u http://dx.doi.org/10.1167/iovs.13-13796 |3 Volltext |
| 912 | |a GBV_USEFLAG_A | ||
| 912 | |a GBV_NLM | ||
| 951 | |a AR | ||
| 952 | |d 55 |j 2014 |e 11 |b 14 |c 10 |h 7535-44 | ||