Mastoparan induces apoptosis in B16F10-Nex2 melanoma cells via the intrinsic mitochondrial pathway and displays antitumor activity in vivo
Copyright © 2014 Elsevier Inc. All rights reserved..
Mastoparan is an α-helical and amphipathic tetradecapeptide obtained from the venom of the wasp Vespula lewisii. This peptide exhibits a wide variety of biological effects, including antimicrobial activity, increased histamine release from mast cells, induction of a potent mitochondrial permeability transition and tumor cell cytotoxicity. Here, the effects of mastoparan in malignant melanoma were studied using the murine model of B16F10-Nex2 cells. In vitro, mastoparan caused melanoma cell death by the mitochondrial apoptosis pathway, as evidenced by the Annexin V-FITC/PI assay, loss of mitochondrial membrane potential (ΔΨm), generation of reactive oxygen species, DNA degradation and cell death signaling. Most importantly, mastoparan reduced the growth of subcutaneous melanoma in syngeneic mice and increased their survival. The present results show that mastoparan induced caspase-dependent apoptosis in melanoma cells through the intrinsic mitochondrial pathway protecting the mice against tumor development.
| Medienart: |
E-Artikel |
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| Erscheinungsjahr: |
2015 |
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| Erschienen: |
2015 |
| Enthalten in: |
Zur Gesamtaufnahme - volume:68 |
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| Enthalten in: |
Peptides - 68(2015) vom: 15. Juni, Seite 113-9 |
| Sprache: |
Englisch |
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| Beteiligte Personen: |
de Azevedo, Ricardo A [VerfasserIn] |
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| Links: |
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| Anmerkungen: |
Date Completed 01.03.2016 Date Revised 10.12.2019 published: Print-Electronic Citation Status MEDLINE |
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| doi: |
10.1016/j.peptides.2014.09.024 |
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| funding: |
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| Förderinstitution / Projekttitel: |
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| PPN (Katalog-ID): |
NLM242689728 |
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| 100 | 1 | |a de Azevedo, Ricardo A |e verfasserin |4 aut | |
| 245 | 1 | 0 | |a Mastoparan induces apoptosis in B16F10-Nex2 melanoma cells via the intrinsic mitochondrial pathway and displays antitumor activity in vivo |
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| 500 | |a Date Revised 10.12.2019 | ||
| 500 | |a published: Print-Electronic | ||
| 500 | |a Citation Status MEDLINE | ||
| 520 | |a Copyright © 2014 Elsevier Inc. All rights reserved. | ||
| 520 | |a Mastoparan is an α-helical and amphipathic tetradecapeptide obtained from the venom of the wasp Vespula lewisii. This peptide exhibits a wide variety of biological effects, including antimicrobial activity, increased histamine release from mast cells, induction of a potent mitochondrial permeability transition and tumor cell cytotoxicity. Here, the effects of mastoparan in malignant melanoma were studied using the murine model of B16F10-Nex2 cells. In vitro, mastoparan caused melanoma cell death by the mitochondrial apoptosis pathway, as evidenced by the Annexin V-FITC/PI assay, loss of mitochondrial membrane potential (ΔΨm), generation of reactive oxygen species, DNA degradation and cell death signaling. Most importantly, mastoparan reduced the growth of subcutaneous melanoma in syngeneic mice and increased their survival. The present results show that mastoparan induced caspase-dependent apoptosis in melanoma cells through the intrinsic mitochondrial pathway protecting the mice against tumor development | ||
| 650 | 4 | |a Journal Article | |
| 650 | 4 | |a Research Support, Non-U.S. Gov't | |
| 650 | 4 | |a Antitumor effects in vivo | |
| 650 | 4 | |a Apoptosis | |
| 650 | 4 | |a Intrinsic pathway | |
| 650 | 4 | |a Mastoparan | |
| 650 | 4 | |a Melanoma | |
| 650 | 7 | |a Antineoplastic Agents |2 NLM | |
| 650 | 7 | |a Intercellular Signaling Peptides and Proteins |2 NLM | |
| 650 | 7 | |a Peptides |2 NLM | |
| 650 | 7 | |a Wasp Venoms |2 NLM | |
| 650 | 7 | |a mastoparan |2 NLM | |
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| 700 | 1 | |a Figueiredo, Carlos R |e verfasserin |4 aut | |
| 700 | 1 | |a Ferreira, Adilson K |e verfasserin |4 aut | |
| 700 | 1 | |a Matsuo, Alisson L |e verfasserin |4 aut | |
| 700 | 1 | |a Massaoka, Mariana H |e verfasserin |4 aut | |
| 700 | 1 | |a Girola, Natalia |e verfasserin |4 aut | |
| 700 | 1 | |a Auada, Aline V V |e verfasserin |4 aut | |
| 700 | 1 | |a Farias, Camyla F |e verfasserin |4 aut | |
| 700 | 1 | |a Pasqualoto, Kerly F M |e verfasserin |4 aut | |
| 700 | 1 | |a Rodrigues, Cecília P |e verfasserin |4 aut | |
| 700 | 1 | |a Barbuto, José A |e verfasserin |4 aut | |
| 700 | 1 | |a Levy, Debora |e verfasserin |4 aut | |
| 700 | 1 | |a Bydlowski, Sérgio P |e verfasserin |4 aut | |
| 700 | 1 | |a de Sá-Junior, Paulo L |e verfasserin |4 aut | |
| 700 | 1 | |a Travassos, Luiz R |e verfasserin |4 aut | |
| 700 | 1 | |a Lebrun, Ivo |e verfasserin |4 aut | |
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